Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1975-01

AUTHORS

J. Anné, J. F. Peberdy

ABSTRACT

Solutions containing polyethylene glycol MW 6000 (PEG) induced fusion of protoplasts of Penicillium chrysogenum. Balanced heterokaryons were formed by fusion of nutritionally complementing protoplasts. Heterokaryotic fusion products were obtained up to a frequency of 4% of the number of protoplasts, surviving the fusion treatment. Investigation of the conditions, necessary to achieve this high fusion frequency, showed that supplementing the PEG solution with Ca++ and adjustment to high pH gave the best results. Mechanisms of fusion of fungal protoplasts by PEG, calcium and alkaline pH are discussed in view of the obtained results. More... »

PAGES

201-205

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00447138

DOI

http://dx.doi.org/10.1007/bf00447138

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049095245

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/242297


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Aggregation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Fusion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Magnesium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Penicillium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Penicillium chrysogenum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polyethylene Glycols", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protoplasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Botany, University of Nottingham, Nottingham", 
          "id": "http://www.grid.ac/institutes/grid.4563.4", 
          "name": [
            "The Rega Institute, University of Leuven, Leuven", 
            "Department of Botany, University of Nottingham, Nottingham"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ann\u00e9", 
        "givenName": "J.", 
        "id": "sg:person.01103102022.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103102022.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Botany, University of Nottingham, Nottingham", 
          "id": "http://www.grid.ac/institutes/grid.4563.4", 
          "name": [
            "The Rega Institute, University of Leuven, Leuven", 
            "Department of Botany, University of Nottingham, Nottingham"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Peberdy", 
        "givenName": "J. F.", 
        "id": "sg:person.014724334325.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014724334325.36"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/253194a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014338258", 
          "https://doi.org/10.1038/253194a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00390290", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038926683", 
          "https://doi.org/10.1007/bf00390290"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00268622", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025943245", 
          "https://doi.org/10.1007/bf00268622"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio242215a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038409732", 
          "https://doi.org/10.1038/newbio242215a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00425278", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029867527", 
          "https://doi.org/10.1007/bf00425278"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00388618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024300197", 
          "https://doi.org/10.1007/bf00388618"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1975-01", 
    "datePublishedReg": "1975-01-01", 
    "description": "Solutions containing polyethylene glycol MW 6000 (PEG) induced fusion of protoplasts of Penicillium chrysogenum. Balanced heterokaryons were formed by fusion of nutritionally complementing protoplasts. Heterokaryotic fusion products were obtained up to a frequency of 4% of the number of protoplasts, surviving the fusion treatment. Investigation of the conditions, necessary to achieve this high fusion frequency, showed that supplementing the PEG solution with Ca++ and adjustment to high pH gave the best results. Mechanisms of fusion of fungal protoplasts by PEG, calcium and alkaline pH are discussed in view of the obtained results.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00447138", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018945", 
        "issn": [
          "0302-8933", 
          "1432-072X"
        ], 
        "name": "Archives of Microbiology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "105"
      }
    ], 
    "keywords": [
      "fusion frequency", 
      "high fusion frequency", 
      "fusion treatment", 
      "polyethylene glycol solution", 
      "treatment", 
      "mechanism of fusion", 
      "calcium", 
      "polyethylene glycol Mw 6000", 
      "fusion", 
      "fusion products", 
      "frequency", 
      "adjustment", 
      "better results", 
      "induced fusion", 
      "results", 
      "mechanism", 
      "PEG solution", 
      "number", 
      "conditions", 
      "alkaline pH", 
      "investigation", 
      "glycol solution", 
      "number of protoplasts", 
      "High", 
      "Penicillium chrysogenum", 
      "products", 
      "pH", 
      "view", 
      "heterokaryons", 
      "fungal protoplasts", 
      "fusion of protoplasts", 
      "balanced heterokaryons", 
      "protoplasts", 
      "chrysogenum", 
      "solution"
    ], 
    "name": "Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions", 
    "pagination": "201-205", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1049095245"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00447138"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "242297"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00447138", 
      "https://app.dimensions.ai/details/publication/pub.1049095245"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-09-02T15:45", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_149.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00447138"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00447138'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00447138'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00447138'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00447138'


 

This table displays all metadata directly associated to this object as RDF triples.

176 TRIPLES      21 PREDICATES      79 URIs      63 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00447138 schema:about N08774435d41248a88ffbdc8c734de4a5
2 N0e783d60c6084d6b819c324910603121
3 N254557abdf2f4570b4ba04641cd4d091
4 N307e8ab250ef4076a7507f975d58e371
5 N32b5c0b6581e42a29c5e33f8a90fdcc3
6 N6b5a53716630495bbfd5316675a59d7f
7 N6bdd59c9dab0444c80c973a5193d159b
8 N74d0ac15059d45bd9cacefb782e8484e
9 N99e9d8adce3d4f969fff95e081fa7dd0
10 Nd646d744080a48cbaf65cf6509a9efbe
11 anzsrc-for:06
12 anzsrc-for:0605
13 anzsrc-for:11
14 anzsrc-for:1108
15 schema:author Nf9c9c1cd2ef64c209309a8cb32d9016a
16 schema:citation sg:pub.10.1007/bf00268622
17 sg:pub.10.1007/bf00388618
18 sg:pub.10.1007/bf00390290
19 sg:pub.10.1007/bf00425278
20 sg:pub.10.1038/253194a0
21 sg:pub.10.1038/newbio242215a0
22 schema:datePublished 1975-01
23 schema:datePublishedReg 1975-01-01
24 schema:description Solutions containing polyethylene glycol MW 6000 (PEG) induced fusion of protoplasts of Penicillium chrysogenum. Balanced heterokaryons were formed by fusion of nutritionally complementing protoplasts. Heterokaryotic fusion products were obtained up to a frequency of 4% of the number of protoplasts, surviving the fusion treatment. Investigation of the conditions, necessary to achieve this high fusion frequency, showed that supplementing the PEG solution with Ca++ and adjustment to high pH gave the best results. Mechanisms of fusion of fungal protoplasts by PEG, calcium and alkaline pH are discussed in view of the obtained results.
25 schema:genre article
26 schema:isAccessibleForFree false
27 schema:isPartOf N6d204c0cdad54ee1ad9e4f786126ecc8
28 Nce0955dd4a1c42b183450ba64120f26a
29 sg:journal.1018945
30 schema:keywords High
31 PEG solution
32 Penicillium chrysogenum
33 adjustment
34 alkaline pH
35 balanced heterokaryons
36 better results
37 calcium
38 chrysogenum
39 conditions
40 frequency
41 fungal protoplasts
42 fusion
43 fusion frequency
44 fusion of protoplasts
45 fusion products
46 fusion treatment
47 glycol solution
48 heterokaryons
49 high fusion frequency
50 induced fusion
51 investigation
52 mechanism
53 mechanism of fusion
54 number
55 number of protoplasts
56 pH
57 polyethylene glycol Mw 6000
58 polyethylene glycol solution
59 products
60 protoplasts
61 results
62 solution
63 treatment
64 view
65 schema:name Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions
66 schema:pagination 201-205
67 schema:productId N411fbad85b6f428d9a360f1cdf7b828f
68 N5c32b888d23040008b43d1e2ed2111cf
69 Nb7141037955e478f87a7e6d6e5de77f3
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049095245
71 https://doi.org/10.1007/bf00447138
72 schema:sdDatePublished 2022-09-02T15:45
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher N962d8d32952142a2a0b71fa55378cd50
75 schema:url https://doi.org/10.1007/bf00447138
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N08774435d41248a88ffbdc8c734de4a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Cell Fusion
81 rdf:type schema:DefinedTerm
82 N0e783d60c6084d6b819c324910603121 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Hydrogen-Ion Concentration
84 rdf:type schema:DefinedTerm
85 N254557abdf2f4570b4ba04641cd4d091 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Polyethylene Glycols
87 rdf:type schema:DefinedTerm
88 N307e8ab250ef4076a7507f975d58e371 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Penicillium chrysogenum
90 rdf:type schema:DefinedTerm
91 N32b5c0b6581e42a29c5e33f8a90fdcc3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Magnesium
93 rdf:type schema:DefinedTerm
94 N411fbad85b6f428d9a360f1cdf7b828f schema:name dimensions_id
95 schema:value pub.1049095245
96 rdf:type schema:PropertyValue
97 N5c32b888d23040008b43d1e2ed2111cf schema:name doi
98 schema:value 10.1007/bf00447138
99 rdf:type schema:PropertyValue
100 N6b5a53716630495bbfd5316675a59d7f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Calcium
102 rdf:type schema:DefinedTerm
103 N6bdd59c9dab0444c80c973a5193d159b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Cell Aggregation
105 rdf:type schema:DefinedTerm
106 N6d204c0cdad54ee1ad9e4f786126ecc8 schema:volumeNumber 105
107 rdf:type schema:PublicationVolume
108 N74d0ac15059d45bd9cacefb782e8484e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Temperature
110 rdf:type schema:DefinedTerm
111 N764375669d4d49139085884c79746e01 rdf:first sg:person.014724334325.36
112 rdf:rest rdf:nil
113 N962d8d32952142a2a0b71fa55378cd50 schema:name Springer Nature - SN SciGraph project
114 rdf:type schema:Organization
115 N99e9d8adce3d4f969fff95e081fa7dd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Penicillium
117 rdf:type schema:DefinedTerm
118 Nb7141037955e478f87a7e6d6e5de77f3 schema:name pubmed_id
119 schema:value 242297
120 rdf:type schema:PropertyValue
121 Nce0955dd4a1c42b183450ba64120f26a schema:issueNumber 1
122 rdf:type schema:PublicationIssue
123 Nd646d744080a48cbaf65cf6509a9efbe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Protoplasts
125 rdf:type schema:DefinedTerm
126 Nf9c9c1cd2ef64c209309a8cb32d9016a rdf:first sg:person.01103102022.24
127 rdf:rest N764375669d4d49139085884c79746e01
128 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
129 schema:name Biological Sciences
130 rdf:type schema:DefinedTerm
131 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
132 schema:name Microbiology
133 rdf:type schema:DefinedTerm
134 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
135 schema:name Medical and Health Sciences
136 rdf:type schema:DefinedTerm
137 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
138 schema:name Medical Microbiology
139 rdf:type schema:DefinedTerm
140 sg:journal.1018945 schema:issn 0302-8933
141 1432-072X
142 schema:name Archives of Microbiology
143 schema:publisher Springer Nature
144 rdf:type schema:Periodical
145 sg:person.01103102022.24 schema:affiliation grid-institutes:grid.4563.4
146 schema:familyName Anné
147 schema:givenName J.
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103102022.24
149 rdf:type schema:Person
150 sg:person.014724334325.36 schema:affiliation grid-institutes:grid.4563.4
151 schema:familyName Peberdy
152 schema:givenName J. F.
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014724334325.36
154 rdf:type schema:Person
155 sg:pub.10.1007/bf00268622 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025943245
156 https://doi.org/10.1007/bf00268622
157 rdf:type schema:CreativeWork
158 sg:pub.10.1007/bf00388618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024300197
159 https://doi.org/10.1007/bf00388618
160 rdf:type schema:CreativeWork
161 sg:pub.10.1007/bf00390290 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038926683
162 https://doi.org/10.1007/bf00390290
163 rdf:type schema:CreativeWork
164 sg:pub.10.1007/bf00425278 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029867527
165 https://doi.org/10.1007/bf00425278
166 rdf:type schema:CreativeWork
167 sg:pub.10.1038/253194a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014338258
168 https://doi.org/10.1038/253194a0
169 rdf:type schema:CreativeWork
170 sg:pub.10.1038/newbio242215a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038409732
171 https://doi.org/10.1038/newbio242215a0
172 rdf:type schema:CreativeWork
173 grid-institutes:grid.4563.4 schema:alternateName Department of Botany, University of Nottingham, Nottingham
174 schema:name Department of Botany, University of Nottingham, Nottingham
175 The Rega Institute, University of Leuven, Leuven
176 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...