Transformation of Saccharomyces cerevisiae with plasmids containing fragments of yeast 2 μ DNA and a suppressor tRNA gene View Full Text


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Article Info

DATE

1980-07

AUTHORS

David Y. Thomas, Allen P. James

ABSTRACT

Hybrid plasmids have been constructed containing segments of the yeast plasmid 2 μ DNA, the yeast ochre-suppressing SUP4.0 gene and the bacterial plasmid pBR322. Yeast transformation is detected with a host containing multiple ochre auxotrophic mutations. The transformed SUP4.0 gene is active and can promote growth in the absence of all the requirements. Plasmids containing different fragments of 2 μ DNA all appear to be active in high frequency transformation of yeast containing 2 μ DNA, except those containing the HindlII-D fragment. The transforming plasmids undergo recombination with the indigenous 2 μ DNA. Integration of the transforming plasmid into the host chromosome has been detected by hybridization of restriction enzyme cleaved DNA with labelled pBR322. The plasmids contain restriction enzyme sites which can be used for cloning other genes into yeast. More... »

PAGES

9-16

References to SciGraph publications

  • 1978-09. Transformation of yeast by a replicating hybrid plasmid in NATURE
  • 1974. Suppressors in Yeast in MODERN ASPECTS OF ELECTROCHEMISTRY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00445689

    DOI

    http://dx.doi.org/10.1007/bf00445689

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015150873

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24189718


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