Production, purification and properties of a Pichia kluyveri killer toxin View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-09

AUTHORS

E. J. Middelbeek, J. M. H. Hermans, C. Stumm

ABSTRACT

Production of the killer toxin of Pichia kluyveri 1002 was stimulated in the presence of yeast extract. In a minimal medium production was optimal at pH 3.8-4.0 and 22--25 degrees C. Addition of gelatin and nonionic detergents, like Brij-58 (polyoxyethylene 20 cetyl ether) and Triton-X-100, to this medium enhanced production significantly. The killer toxin was purified 140-fold by use of a stepwise ethanol precipitation and butyl Sepharose column chromatography. The purified killer toxin, which still contained some carbohydrates, appeared to be glycoprotein with a mol wt of about 19 000 and an isoelectric point of 4.3. It was stable between pH 2.5 and 4.7 and up to 40 degrees C. More... »

PAGES

437-450

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00443282

DOI

http://dx.doi.org/10.1007/bf00443282

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003743482

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/45295


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