Overlapping sequences of Klebsiella pneumoniae nif DNA cloned and characterised View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-01

AUTHORS

Frank C. Cannon, Gerard E. Riedel, Fredrick M. Ausubel

ABSTRACT

A HindIII (17.0 kb) and an EcoR1 restriction fragment (6.9 kb) of Klebsiella pneumoniae nif DNA were cloned on two small amplifiable plasmids, pCM1 and pSA30 respectively. These plasmids between them carry 14 of the 15 known Klebsiella nif genes. The operon for the three structural genes for nitrogenase, nifpHDK, is carried on pSA30: four and five of the remaining six operons are on pCRA37 and pCM1 respectively. All of the nif genes were assigned to endonuclease restriction fragments of DNA using the Southern blotting technique (Southern, 1975) with total DNA of nif insertion mutants and radioactive plasmid DNA which contained cloned nif DNA sequences. Their locations were consistent with the genetic map of nif genes. The estimated size of the nif gene cluster was 24 kb. More... »

PAGES

59-66

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00433306

DOI

http://dx.doi.org/10.1007/bf00433306

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046705218

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/384162


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