Physical mapping of BglII, BamHI, EcoRI, HindIII and PstI Restriction fragments of bacteriophage P1 DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1977-01

AUTHORS

Brigitte Bächi, Werner Arber

ABSTRACT

A cleavage map of bacteriophage P1 DNA was established by reciprocal double digestion with various restriction endonucleases. The enzymes used and, in parenthesis, the number of their cleavage sites on the P1clts genome are: PstI (1), HindIII(3), BglII (11), BamHI (14) and EcoRI (26). The relative order of the PstI, HindIII and BglII sites, as well as the order of 13 out of the 14 BamHI sites and of 17 out of the 26 EcoRI sites was determined. The P1 genome was divided into 100 map units and the PstI site was arbitrarily chosen as reference point at map unit 20. DNA packaging into phage heads starts preferentially at map unit 92 and it proceeds towards higher map units. The two inverted repeat sequences of P1 DNA map about at units 30 and 34. More... »

PAGES

311-324

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00431596

DOI

http://dx.doi.org/10.1007/bf00431596

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045779798

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/895712


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