Genetic transformation of oilseed rape (Brassica napus) by the Ri T-DNA of Agrobacterium rhizogenes and analysis of inheritance of the ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-03

AUTHORS

P. Guerche, L. Jouanin, D. Tepfer, G. Pelletier

ABSTRACT

Genetically transformed repeseed (Brassica napus) roots were obtained by in vitro inoculation of excised stem segments with Agrobacterium rhizogenes. Axenic root organ clones were established and they exhibited a phenotype characteristic of transformed roots: rapid growth, reduced apical dominance and root plagiotropism. Stem regeneration was induced by exposing root fragments to 2,4-dichloroacetic acid (2,4-D) in liquid medium, followed by transfer to solid regeneration medium. The resulting plants exhibited the transformed phenotype observed in other species where similar experiments have been performed. Direct evidence for genetic transformation was obtained from opine assays and molecular hybridization. Sexual transmission of the transformed phenotype was Mendelian, and a probable case of T-DNA insertion into two independent loci within the same plant was detected. The estimated optimal time necessary to obtain transformed oilseed rape plants using this approach is 2 months. More... »

PAGES

382-386

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00428875

DOI

http://dx.doi.org/10.1007/bf00428875

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040573711


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