The glucan-chitin complex in Saccharomyces cerevisiae View Full Text


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Article Info

DATE

1981-12

AUTHORS

Zdeněk Holan, Vladimír Pokorný, Karel Beran, Antonín Gemperle, Zdeněk Tuzar, Josef Baldrián

ABSTRACT

Scar rings (SR) from scarless cells at the early stages of budding and mature bud scars from Saccharomyces cerevisiae isolated by both chemical and enzymic treatment of cell walls were observed by selected-area electron diffraction (SAED), X-ray diffraction and electron microscopy with simultaneous physico-chemical characterization (including molar mass, intrinsic viscosity and crystallite size) of α-chitin and glucan. The SR, composed of glucan with strong 0.608; 0.397 and 0.293 nm X-ray reflections, was formed at the start of budding. The SAED patterns of α-chitin both in the adjacent circular zone and in the parts of newly formed primary septum (PS), observed when the development of the PS started, did not differ from those of α-chitin in the single mature bud scar. The bud scar consisted of α-chitin, glucan and mannan and their content, as well as the crystallite size of chitin, depended on the mode of preparation. More... »

PAGES

312-318

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00425946

DOI

http://dx.doi.org/10.1007/bf00425946

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023723343


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