Characterization of 3H-labeled supercoiled mitochondrial DNA from tobacco suspension culture cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1980-11

AUTHORS

R. B. Sparks, R. M. K. Dale

ABSTRACT

Mitochondrial DNA of tobacco supension culture cells labeled in vivo with 3H-thymidine was isolated from purified mitochondria. It has a density of 1.707±0.002 g/cm3 unique from both nuclear and chloroplast DNA. Approximately 25–45% of the mtDNA molecules from several preparations from suspension culture cells were isolated as supercoiled molecules from ethidium bromide-cesium chloride gradients. Electron microscopic examination of nicked supercoiled molecules revealed a heterogenous population of molecules ranging in size from 0.5 to 42 μm. The most abundant size class of 365 molecules measured was 9 μm. Restriction endonuclease digests of both supercoiled and open circular/linear molecules were essentially identical and yielded a minimum size estimate of 76 μm. The availability of large amounts of labeled covalently closed circular molecules will facilitate the resolution of the different size classes and allow, for the first time, a direct analysis of the relationships among the heterogenous population of DNA molecules found in plant mitochondria. More... »

PAGES

351-355

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00425847

DOI

http://dx.doi.org/10.1007/bf00425847

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https://app.dimensions.ai/details/publication/pub.1010175739


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