Transposon mutagenesis of Erwinia using phage λ vectors View Full Text


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Article Info

DATE

1986-06

AUTHORS

George P. C. Salmond, Jay C. D. Hinton, Deborah R. Gill, Michel C. M. Pérombelon

ABSTRACT

The plasmid pHCP2 carries the lamB gene of E. coli. This plasmid was transferred to 4 strains of Erwinia using transformation or conjugal mobilisation. All 4 plasmid-containing strains became sensitive to λ infection. After infection with λ::Tn5 and the “higher-hopper” Tn10 derivatives, a variety of auxotrophic and enzyme-deficient mutants were isolated. Reversion analysis suggested the mutant phenotypes were due to the transposon. More... »

PAGES

524-528

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00422080

DOI

http://dx.doi.org/10.1007/bf00422080

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052980834


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