Physical mapping, nucleotide sequencing and expression in E. coli minicells of the gene for the large subunit of ribulose bisphosphate ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1984-04

AUTHORS

W. A. Bovenberg, R. E. Koes, A. J. Kool, H. J. J. Nijkamp

ABSTRACT

The Petunia hybrida rbcL gene was identified and located on the physical map within the Sall S9 fragment of the Petunia hybrida cpDNA by heterologous hybridization with the cloned rbcL gene of spinach (pSoc3BE148). In E. coli minicells harbouring the S9 fragment inserted into pBR322, the rbcL polypeptide is synthesized as was shown by molecular weight determination, immunoprecipitation and proteolytic digestion. However, the size of the rbcL polypeptide synthesized in minicells appeared to be dependent on the orientation of the S9 fragment in pBR322. In minicells harbouring the S9 fragment inserted into pBR322 in the clockwise orientation the molecular weight of the rbcL polypeptide is approximately 53 kD, whereas in minicells harbouring the S9 fragment in the opposite orientation, the rbcL polypeptide synthesized has a molecular weight of 52 kD. The difference in molecular weight of the two rbcL polypeptides is the result of transcription and translation into the flanking pBR322 sequences. This is due to the absence of the terminal part (6 codons), including the translation stop codon, of the rbcL gene on the cloned S9 fragment as was determined by nucleotide sequencing. The observed expression of the cloned part of the rbcL gene of Petunia hybrida indicates that the E. coli minicell system can be used as a suitable and convenient system for the identification and physical mapping of chloroplast genes.Comparison of the sequence of the untranslated 3'-end of the rbcL gene of Petunia hybrida with that of Nicotiana tabacum revealed a striking similarity of the region in which stem and loop structures can be formed that are most likely involved in termination of transcription of the rbcL gene. This region appears to be highly conserved in the rbcL genes of P. hybrida, N. tabacum, S. oleracea and Z. mays. More... »

PAGES

231-241

References to SciGraph publications

  • 1980-12. Chloroplast gene sequence for the large subunit of ribulose bisphosphatecarboxylase of maize in NATURE
  • 1982-12. Restriction endonuclease analysis of chloroplast DNA in interspecies somatic Hybrids of Petunia in THEORETICAL AND APPLIED GENETICS
  • 1973-11. Improved Estimation of Secondary Structure in Ribonucleic Acids in NATURE
  • 1982-05. Amplification of maize ribulose bisphosphate carboxylase large subunit synthesis in E. coli by transcriptional fusion with the lambda N operon in MOLECULAR GENETICS AND GENOMICS
  • 1983-03. Chloroplast DNAs of Spinacia, Petunia and Spirodela have a similar gene organization in CURRENT GENETICS
  • 1981-07. Comments on the structure and function of the large subunit of the enzyme ribulose bisphosphate carboxylase-oxygenase in CARLSBERG RESEARCH COMMUNICATIONS
  • 1981-07. A physical map of Nicotiana tabacum plastid DNA including the location of structural genes for ribosomal RNAs and the large subunit of ribulose bisphosphate carboxylase/oxygenase in CURRENT GENETICS
  • 1981-05. Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase in NATURE
  • 1982-11. Light and benzylaminopurine induce changes in ultrastructure and gene expression in plastids of Petunia hybrida cell cultures in CURRENT GENETICS
  • 1980. Fraction I Protein in CHLOROPLASTS
  • 1982. Nucleic Acids and Proteins in Plants II, Structure, Biochemistry and Physiology of Nucleic Acids in NONE
  • 1980. Chloroplasts in NONE
  • 1983-04. Plastid gene expression in a yellow-green leaf mutant of Petunia hybrida in PLANTA
  • 1972-12. Proteins synthesized by a non-induced bacteriocinogenic factor in minicells of Escherichia coli in MOLECULAR GENETICS AND GENOMICS
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 1982. The Biosynthesis of Chloroplast Proteins in NUCLEIC ACIDS AND PROTEINS IN PLANTS II
  • Journal

    TITLE

    Current Genetics

    ISSUE

    3

    VOLUME

    8

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00417821

    DOI

    http://dx.doi.org/10.1007/bf00417821

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1023165632

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24177744


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "VU University Amsterdam", 
              "id": "https://www.grid.ac/institutes/grid.12380.38", 
              "name": [
                "Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bovenberg", 
            "givenName": "W. A.", 
            "id": "sg:person.01015700113.46", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015700113.46"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "VU University Amsterdam", 
              "id": "https://www.grid.ac/institutes/grid.12380.38", 
              "name": [
                "Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Koes", 
            "givenName": "R. E.", 
            "id": "sg:person.01007106747.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007106747.18"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "VU University Amsterdam", 
              "id": "https://www.grid.ac/institutes/grid.12380.38", 
              "name": [
                "Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kool", 
            "givenName": "A. J.", 
            "id": "sg:person.01346105067.73", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346105067.73"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "VU University Amsterdam", 
              "id": "https://www.grid.ac/institutes/grid.12380.38", 
              "name": [
                "Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nijkamp", 
            "givenName": "H. J. J.", 
            "id": "sg:person.01010106014.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010106014.17"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00365673", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000133883", 
              "https://doi.org/10.1007/bf00365673"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(82)90090-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000617160"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(82)90090-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000617160"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1979.tb12791.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001817862"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-2697(79)90716-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004142387"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1977.tb11296.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004596288"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.ge.13.120179.001535", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005203403"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(81)90040-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006086769"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(81)90040-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006086769"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0045-6039(78)90014-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009287362"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0045-6039(78)90014-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009287362"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/288556a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010023964", 
              "https://doi.org/10.1038/288556a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00405184", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010230691", 
              "https://doi.org/10.1007/bf00405184"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00405184", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010230691", 
              "https://doi.org/10.1007/bf00405184"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/227680a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010419937", 
              "https://doi.org/10.1038/227680a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.62.4.1159", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011113885"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.76.9.4350", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011704795"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.77.4.1832", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013058078"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/291117a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015356653", 
              "https://doi.org/10.1038/291117a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(79)90082-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015414693"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(79)90082-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015414693"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/5.12.4537", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015518834"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-540-38255-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015608364", 
              "https://doi.org/10.1007/978-3-540-38255-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-540-38255-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015608364", 
              "https://doi.org/10.1007/978-3-540-38255-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00429821", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018169665", 
              "https://doi.org/10.1007/bf00429821"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0045-6039(75)90040-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018194153"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0045-6039(75)90040-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018194153"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0076-6879(79)68018-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018959037"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/newbio246040a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018988773", 
              "https://doi.org/10.1038/newbio246040a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/newbio246040a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018988773", 
              "https://doi.org/10.1038/newbio246040a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-2836(74)90357-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019434826"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0076-6879(79)68038-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019646517"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.74.2.560", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021800596"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/10.6.1913", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022709085"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-2836(69)90288-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023146115"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/9.14.3251", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023866249"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-540-38255-3_6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027651743", 
              "https://doi.org/10.1007/978-3-540-38255-3_6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00435212", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028792814", 
              "https://doi.org/10.1007/bf00435212"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033296365"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00333170", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034145116", 
              "https://doi.org/10.1007/bf00333170"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-68347-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036002904", 
              "https://doi.org/10.1007/978-3-642-68347-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-68347-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036002904", 
              "https://doi.org/10.1007/978-3-642-68347-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1982.tb05842.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038533624"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/9.3.503", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040749781"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.pp.32.060181.000551", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042300193"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(77)90000-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044383550"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/7.6.1513", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046335196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-9861(79)90313-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047709946"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00334134", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049714925", 
              "https://doi.org/10.1007/bf00334134"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-68347-3_15", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049792984", 
              "https://doi.org/10.1007/978-3-642-68347-3_15"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02906502", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053233272", 
              "https://doi.org/10.1007/bf02906502"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02906502", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053233272", 
              "https://doi.org/10.1007/bf02906502"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/sqb.1979.043.01.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060404824"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00275109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078817981", 
              "https://doi.org/10.1007/bf00275109"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00275109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078817981", 
              "https://doi.org/10.1007/bf00275109"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079609947", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079928128", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080463747", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080826031", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081649793", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1984-04", 
        "datePublishedReg": "1984-04-01", 
        "description": "The Petunia hybrida rbcL gene was identified and located on the physical map within the Sall S9 fragment of the Petunia hybrida cpDNA by heterologous hybridization with the cloned rbcL gene of spinach (pSoc3BE148). In E. coli minicells harbouring the S9 fragment inserted into pBR322, the rbcL polypeptide is synthesized as was shown by molecular weight determination, immunoprecipitation and proteolytic digestion. However, the size of the rbcL polypeptide synthesized in minicells appeared to be dependent on the orientation of the S9 fragment in pBR322. In minicells harbouring the S9 fragment inserted into pBR322 in the clockwise orientation the molecular weight of the rbcL polypeptide is approximately 53 kD, whereas in minicells harbouring the S9 fragment in the opposite orientation, the rbcL polypeptide synthesized has a molecular weight of 52 kD. The difference in molecular weight of the two rbcL polypeptides is the result of transcription and translation into the flanking pBR322 sequences. This is due to the absence of the terminal part (6 codons), including the translation stop codon, of the rbcL gene on the cloned S9 fragment as was determined by nucleotide sequencing. The observed expression of the cloned part of the rbcL gene of Petunia hybrida indicates that the E. coli minicell system can be used as a suitable and convenient system for the identification and physical mapping of chloroplast genes.Comparison of the sequence of the untranslated 3'-end of the rbcL gene of Petunia hybrida with that of Nicotiana tabacum revealed a striking similarity of the region in which stem and loop structures can be formed that are most likely involved in termination of transcription of the rbcL gene. This region appears to be highly conserved in the rbcL genes of P. hybrida, N. tabacum, S. oleracea and Z. mays. ", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00417821", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1091193", 
            "issn": [
              "0172-8083", 
              "1432-0983"
            ], 
            "name": "Current Genetics", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "8"
          }
        ], 
        "name": "Physical mapping, nucleotide sequencing and expression in E. coli minicells of the gene for the large subunit of ribulose bisphosphate carboxylase from Petunia hybrida", 
        "pagination": "231-241", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "6e5de0cc6c692c044f382397da903588bfe2a6491a6da5b31baa20f45a7c4106"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "24177744"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "8004904"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00417821"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1023165632"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00417821", 
          "https://app.dimensions.ai/details/publication/pub.1023165632"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:55", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130814_00000002.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00417821"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00417821'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00417821'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00417821'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00417821'


     

    This table displays all metadata directly associated to this object as RDF triples.

    248 TRIPLES      21 PREDICATES      78 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00417821 schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author Nb54c116211fc41b884c545c483fd95e6
    4 schema:citation sg:pub.10.1007/978-3-540-38255-3
    5 sg:pub.10.1007/978-3-540-38255-3_6
    6 sg:pub.10.1007/978-3-642-68347-3
    7 sg:pub.10.1007/978-3-642-68347-3_15
    8 sg:pub.10.1007/bf00275109
    9 sg:pub.10.1007/bf00333170
    10 sg:pub.10.1007/bf00334134
    11 sg:pub.10.1007/bf00365673
    12 sg:pub.10.1007/bf00405184
    13 sg:pub.10.1007/bf00429821
    14 sg:pub.10.1007/bf00435212
    15 sg:pub.10.1007/bf02906502
    16 sg:pub.10.1038/227680a0
    17 sg:pub.10.1038/288556a0
    18 sg:pub.10.1038/291117a0
    19 sg:pub.10.1038/newbio246040a0
    20 https://app.dimensions.ai/details/publication/pub.1079609947
    21 https://app.dimensions.ai/details/publication/pub.1079928128
    22 https://app.dimensions.ai/details/publication/pub.1080463747
    23 https://app.dimensions.ai/details/publication/pub.1080826031
    24 https://app.dimensions.ai/details/publication/pub.1081649793
    25 https://doi.org/10.1016/0003-2697(79)90716-4
    26 https://doi.org/10.1016/0003-9861(79)90313-8
    27 https://doi.org/10.1016/0022-2836(69)90288-5
    28 https://doi.org/10.1016/0022-2836(74)90357-x
    29 https://doi.org/10.1016/0045-6039(75)90040-8
    30 https://doi.org/10.1016/0045-6039(78)90014-3
    31 https://doi.org/10.1016/0076-6879(79)68018-7
    32 https://doi.org/10.1016/0076-6879(79)68038-2
    33 https://doi.org/10.1016/0378-1119(77)90000-2
    34 https://doi.org/10.1016/0378-1119(79)90082-9
    35 https://doi.org/10.1016/0378-1119(81)90040-8
    36 https://doi.org/10.1016/0378-1119(82)90090-7
    37 https://doi.org/10.1016/s0022-2836(75)80083-0
    38 https://doi.org/10.1073/pnas.62.4.1159
    39 https://doi.org/10.1073/pnas.74.2.560
    40 https://doi.org/10.1073/pnas.76.9.4350
    41 https://doi.org/10.1073/pnas.77.4.1832
    42 https://doi.org/10.1093/nar/10.6.1913
    43 https://doi.org/10.1093/nar/5.12.4537
    44 https://doi.org/10.1093/nar/7.6.1513
    45 https://doi.org/10.1093/nar/9.14.3251
    46 https://doi.org/10.1093/nar/9.3.503
    47 https://doi.org/10.1101/sqb.1979.043.01.013
    48 https://doi.org/10.1111/j.1432-1033.1977.tb11296.x
    49 https://doi.org/10.1111/j.1432-1033.1979.tb12791.x
    50 https://doi.org/10.1111/j.1432-1033.1982.tb05842.x
    51 https://doi.org/10.1146/annurev.ge.13.120179.001535
    52 https://doi.org/10.1146/annurev.pp.32.060181.000551
    53 schema:datePublished 1984-04
    54 schema:datePublishedReg 1984-04-01
    55 schema:description The Petunia hybrida rbcL gene was identified and located on the physical map within the Sall S9 fragment of the Petunia hybrida cpDNA by heterologous hybridization with the cloned rbcL gene of spinach (pSoc3BE148). In E. coli minicells harbouring the S9 fragment inserted into pBR322, the rbcL polypeptide is synthesized as was shown by molecular weight determination, immunoprecipitation and proteolytic digestion. However, the size of the rbcL polypeptide synthesized in minicells appeared to be dependent on the orientation of the S9 fragment in pBR322. In minicells harbouring the S9 fragment inserted into pBR322 in the clockwise orientation the molecular weight of the rbcL polypeptide is approximately 53 kD, whereas in minicells harbouring the S9 fragment in the opposite orientation, the rbcL polypeptide synthesized has a molecular weight of 52 kD. The difference in molecular weight of the two rbcL polypeptides is the result of transcription and translation into the flanking pBR322 sequences. This is due to the absence of the terminal part (6 codons), including the translation stop codon, of the rbcL gene on the cloned S9 fragment as was determined by nucleotide sequencing. The observed expression of the cloned part of the rbcL gene of Petunia hybrida indicates that the E. coli minicell system can be used as a suitable and convenient system for the identification and physical mapping of chloroplast genes.Comparison of the sequence of the untranslated 3'-end of the rbcL gene of Petunia hybrida with that of Nicotiana tabacum revealed a striking similarity of the region in which stem and loop structures can be formed that are most likely involved in termination of transcription of the rbcL gene. This region appears to be highly conserved in the rbcL genes of P. hybrida, N. tabacum, S. oleracea and Z. mays.
    56 schema:genre research_article
    57 schema:inLanguage en
    58 schema:isAccessibleForFree false
    59 schema:isPartOf N45294e9d1a2e4b29a458a3aeeab052d6
    60 N5fcbecd583604f68876f61cc4fbe8296
    61 sg:journal.1091193
    62 schema:name Physical mapping, nucleotide sequencing and expression in E. coli minicells of the gene for the large subunit of ribulose bisphosphate carboxylase from Petunia hybrida
    63 schema:pagination 231-241
    64 schema:productId N71b302af77924754bfdf7563837695f1
    65 N7eb677aabac44a9babfcf15f2257b6ff
    66 N8a6a2f059ffe401089b62de7bb3750bd
    67 Nd0a39a516e394affbed84e93b9c524af
    68 Nd71951cdefc34ae296f0bccd5667b47b
    69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023165632
    70 https://doi.org/10.1007/bf00417821
    71 schema:sdDatePublished 2019-04-11T13:55
    72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    73 schema:sdPublisher Naee44f7548384eb3b8e8bcaae56a0ec6
    74 schema:url http://link.springer.com/10.1007/BF00417821
    75 sgo:license sg:explorer/license/
    76 sgo:sdDataset articles
    77 rdf:type schema:ScholarlyArticle
    78 N45294e9d1a2e4b29a458a3aeeab052d6 schema:issueNumber 3
    79 rdf:type schema:PublicationIssue
    80 N4bb9d362362e41fcb7bee7c1bdb86e59 rdf:first sg:person.01007106747.18
    81 rdf:rest N710248db93f845d295731eccd98ea129
    82 N5fcbecd583604f68876f61cc4fbe8296 schema:volumeNumber 8
    83 rdf:type schema:PublicationVolume
    84 N710248db93f845d295731eccd98ea129 rdf:first sg:person.01346105067.73
    85 rdf:rest Na9c74b7c31134b0c870041bb322db0e6
    86 N71b302af77924754bfdf7563837695f1 schema:name doi
    87 schema:value 10.1007/bf00417821
    88 rdf:type schema:PropertyValue
    89 N7eb677aabac44a9babfcf15f2257b6ff schema:name readcube_id
    90 schema:value 6e5de0cc6c692c044f382397da903588bfe2a6491a6da5b31baa20f45a7c4106
    91 rdf:type schema:PropertyValue
    92 N8a6a2f059ffe401089b62de7bb3750bd schema:name dimensions_id
    93 schema:value pub.1023165632
    94 rdf:type schema:PropertyValue
    95 Na9c74b7c31134b0c870041bb322db0e6 rdf:first sg:person.01010106014.17
    96 rdf:rest rdf:nil
    97 Naee44f7548384eb3b8e8bcaae56a0ec6 schema:name Springer Nature - SN SciGraph project
    98 rdf:type schema:Organization
    99 Nb54c116211fc41b884c545c483fd95e6 rdf:first sg:person.01015700113.46
    100 rdf:rest N4bb9d362362e41fcb7bee7c1bdb86e59
    101 Nd0a39a516e394affbed84e93b9c524af schema:name nlm_unique_id
    102 schema:value 8004904
    103 rdf:type schema:PropertyValue
    104 Nd71951cdefc34ae296f0bccd5667b47b schema:name pubmed_id
    105 schema:value 24177744
    106 rdf:type schema:PropertyValue
    107 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    108 schema:name Biological Sciences
    109 rdf:type schema:DefinedTerm
    110 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    111 schema:name Genetics
    112 rdf:type schema:DefinedTerm
    113 sg:journal.1091193 schema:issn 0172-8083
    114 1432-0983
    115 schema:name Current Genetics
    116 rdf:type schema:Periodical
    117 sg:person.01007106747.18 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
    118 schema:familyName Koes
    119 schema:givenName R. E.
    120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007106747.18
    121 rdf:type schema:Person
    122 sg:person.01010106014.17 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
    123 schema:familyName Nijkamp
    124 schema:givenName H. J. J.
    125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010106014.17
    126 rdf:type schema:Person
    127 sg:person.01015700113.46 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
    128 schema:familyName Bovenberg
    129 schema:givenName W. A.
    130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015700113.46
    131 rdf:type schema:Person
    132 sg:person.01346105067.73 schema:affiliation https://www.grid.ac/institutes/grid.12380.38
    133 schema:familyName Kool
    134 schema:givenName A. J.
    135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346105067.73
    136 rdf:type schema:Person
    137 sg:pub.10.1007/978-3-540-38255-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015608364
    138 https://doi.org/10.1007/978-3-540-38255-3
    139 rdf:type schema:CreativeWork
    140 sg:pub.10.1007/978-3-540-38255-3_6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027651743
    141 https://doi.org/10.1007/978-3-540-38255-3_6
    142 rdf:type schema:CreativeWork
    143 sg:pub.10.1007/978-3-642-68347-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036002904
    144 https://doi.org/10.1007/978-3-642-68347-3
    145 rdf:type schema:CreativeWork
    146 sg:pub.10.1007/978-3-642-68347-3_15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049792984
    147 https://doi.org/10.1007/978-3-642-68347-3_15
    148 rdf:type schema:CreativeWork
    149 sg:pub.10.1007/bf00275109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078817981
    150 https://doi.org/10.1007/bf00275109
    151 rdf:type schema:CreativeWork
    152 sg:pub.10.1007/bf00333170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034145116
    153 https://doi.org/10.1007/bf00333170
    154 rdf:type schema:CreativeWork
    155 sg:pub.10.1007/bf00334134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049714925
    156 https://doi.org/10.1007/bf00334134
    157 rdf:type schema:CreativeWork
    158 sg:pub.10.1007/bf00365673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000133883
    159 https://doi.org/10.1007/bf00365673
    160 rdf:type schema:CreativeWork
    161 sg:pub.10.1007/bf00405184 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010230691
    162 https://doi.org/10.1007/bf00405184
    163 rdf:type schema:CreativeWork
    164 sg:pub.10.1007/bf00429821 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018169665
    165 https://doi.org/10.1007/bf00429821
    166 rdf:type schema:CreativeWork
    167 sg:pub.10.1007/bf00435212 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028792814
    168 https://doi.org/10.1007/bf00435212
    169 rdf:type schema:CreativeWork
    170 sg:pub.10.1007/bf02906502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053233272
    171 https://doi.org/10.1007/bf02906502
    172 rdf:type schema:CreativeWork
    173 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
    174 https://doi.org/10.1038/227680a0
    175 rdf:type schema:CreativeWork
    176 sg:pub.10.1038/288556a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010023964
    177 https://doi.org/10.1038/288556a0
    178 rdf:type schema:CreativeWork
    179 sg:pub.10.1038/291117a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015356653
    180 https://doi.org/10.1038/291117a0
    181 rdf:type schema:CreativeWork
    182 sg:pub.10.1038/newbio246040a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018988773
    183 https://doi.org/10.1038/newbio246040a0
    184 rdf:type schema:CreativeWork
    185 https://app.dimensions.ai/details/publication/pub.1079609947 schema:CreativeWork
    186 https://app.dimensions.ai/details/publication/pub.1079928128 schema:CreativeWork
    187 https://app.dimensions.ai/details/publication/pub.1080463747 schema:CreativeWork
    188 https://app.dimensions.ai/details/publication/pub.1080826031 schema:CreativeWork
    189 https://app.dimensions.ai/details/publication/pub.1081649793 schema:CreativeWork
    190 https://doi.org/10.1016/0003-2697(79)90716-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004142387
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1016/0003-9861(79)90313-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047709946
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1016/0022-2836(69)90288-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023146115
    195 rdf:type schema:CreativeWork
    196 https://doi.org/10.1016/0022-2836(74)90357-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019434826
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1016/0045-6039(75)90040-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018194153
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1016/0045-6039(78)90014-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009287362
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1016/0076-6879(79)68018-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018959037
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1016/0076-6879(79)68038-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019646517
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1016/0378-1119(77)90000-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044383550
    207 rdf:type schema:CreativeWork
    208 https://doi.org/10.1016/0378-1119(79)90082-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015414693
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1016/0378-1119(81)90040-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006086769
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1016/0378-1119(82)90090-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000617160
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1073/pnas.62.4.1159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011113885
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1073/pnas.74.2.560 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021800596
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1073/pnas.76.9.4350 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011704795
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1073/pnas.77.4.1832 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013058078
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1093/nar/10.6.1913 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022709085
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1093/nar/5.12.4537 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015518834
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1093/nar/7.6.1513 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046335196
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1093/nar/9.14.3251 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023866249
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1093/nar/9.3.503 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040749781
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1101/sqb.1979.043.01.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060404824
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1111/j.1432-1033.1977.tb11296.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004596288
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1111/j.1432-1033.1979.tb12791.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1001817862
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1111/j.1432-1033.1982.tb05842.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038533624
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1146/annurev.ge.13.120179.001535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005203403
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1146/annurev.pp.32.060181.000551 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042300193
    245 rdf:type schema:CreativeWork
    246 https://www.grid.ac/institutes/grid.12380.38 schema:alternateName VU University Amsterdam
    247 schema:name Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands
    248 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...