The vacuole as the lysosome of the yeast cell View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1967-06

AUTHORS

Philippe Matile, Andreas Wiemken

ABSTRACT

Vacuoles can be isolated by flotation from suspensions of lysed proto-plasts in the presence of Ficoll. The purity of the respective preparations is demonstrated by the complete absence of enzymes of the mitochondria and of the groundplasm, as well as by morphological observations. Hydrolytic enzymes (two acid proteases, p-nitrophenylacetate-esterase, RNase and leucylaminopeptidase) are present in high specific activities in isolated vacuoles. The vacuole therefore represents the lysosome of the yeast cell. More... »

PAGES

148-155

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00408765

DOI

http://dx.doi.org/10.1007/bf00408765

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005194202

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4873367


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