The permeability of electroporated cells and protoplasts of sugar beet View Full Text


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Article Info

DATE

1987-11

AUTHORS

K. Lindsey, M. G. K. Jones

ABSTRACT

A simple method has been developed to determine the changes in permeability of protoplasts and intact cells when electroporated. Cells and protoplasts of sugar beet, Beta vulgaris L., were subjected to electric pulse treatments of different field strengths, pulse number and pulse duration, and the ability to accumulate and retain the hydrophilic dye phenosafranine was determined spectrophotometrically. Results of timecourse studies of phenosafranine accumulation and retention indicated that pores are formed or enlarged rapidly in the plasmamembrane and remain permeable to phenosafranine for relatively long periods; the half-life of the 'pores" was temperaturedependent. Both cells and protoplasts retained the highest levels of phenosafranine when supplied with a series of five rectangular pulses of 50 μs duration and of field strength 2500 V·cm(-1). If these parameters were exceeded, The phenosafranine content was reduced, concomitant with a decline in viability as indicated by fluorescein-diacetate staining, indicating the loss of the integrity of the plasmamembrane. The pattern of accumulation and retention by protoplasts of radioactivity from [(3)H]pABD1, a modified bacterial plasmid, was similar to that of phenosafranine, but uptake of the plasmid by cells was not demonstrated. The mothod can be used to determine conditions for the optimum permeabilization of protoplasts for direct gene transfer. More... »

PAGES

346-355

References to SciGraph publications

  • 1984-12. Permeabilization of Cinchona ledgeriana cells by dimethylsulphoxide. effects on alkaloid release and long-term membrane integrity in PLANT CELL REPORTS
  • 1986-02. Electroporation-mediated infection of tobacco leaf protoplasts with tobacco mosaic virus RNA and cucumber mosaic virus RNA in PLANT CELL REPORTS
  • 1974-06. A procedure for the in vivo determination of enzyme activity in higher plant tissue in PLANTA
  • 1980-10. Phospholipid composition and fatty acid desaturation in the roots of rye during acclimatization of low temperature in PLANTA
  • 1975-12. Effect of electric fields on light-scattering and fluorescence of chromaffin granules in THE JOURNAL OF MEMBRANE BIOLOGY
  • 1985-05. Production of somatic hybrids of moss by electrofusion in MOLECULAR GENETICS AND GENOMICS
  • 1985-08. Fusion characteristics of plant protoplasts in electric fields in PLANTA
  • 1986-02. Stable transformation of maize after gene transfer by electroporation in NATURE
  • 1985-12. High Efficiency Direct Gene Transfer to Plants in NATURE BIOTECHNOLOGY
  • 1982-03. In vitro transformation of plant protoplasts with Ti-plasmid DNA in NATURE
  • 1977-01. Escherichia coli plasmid pBR313 insertion into plant protoplasts and into their nuclei in MOLECULAR GENETICS AND GENOMICS
  • 1976-01. Purification and viability determinations of plant protoplasts in PLANTA
  • 1977-12. Kinetics of permeability changes induced by electric impulses in chromaffin granules in THE JOURNAL OF MEMBRANE BIOLOGY
  • 1986. Electrical breakdown, electropermeabilization and electrofusion in REVIEWS OF PHYSIOLOGY, BIOCHEMISTRY AND PHARMACOLOGY, VOLUME 105
  • 1985-12. Electric field mediated stable transformation of carrot protoplasts with naked DNA in PLANT CELL REPORTS
  • 1988-08. Production of somatic hybrids by electrofusion in Solanum in THEORETICAL AND APPLIED GENETICS
  • Journal

    TITLE

    Planta

    ISSUE

    3

    VOLUME

    172

    Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00398663

    DOI

    http://dx.doi.org/10.1007/bf00398663

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1021531454

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24225918


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    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

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    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00398663'


     

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