A specific radioimmunoassay for nanogram quantities of the auxin, indole-3-acetic acid View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1977-01

AUTHORS

William Pengelly, Frederick Meins

ABSTRACT

We have developed a specific radioimmunoassay [RIA] for indole-3-acetic acid (IAA) in the 0.2 ng to 12 ng range which, in principle, can be extended to other indole auxins as well. Methods are presented for obtaining suitable antibody, for the RIA procedure, and for measuring IAA in methanolic extracts of plant tissues. Antibody specific for IAA was obtained from rabbits immunized with IAA bound to bovine serum albumin by formaldehyde treatment. In assays with this antibody, 2,4-dichlorophenoxyacetic acid and indoles structurally related to IAA reacted from 300- to 3000-fold less than did IAA itself. However, α-and β-naphthaleneacetic acid reacted significantly and hence interfered with the assay. Extracts of tobacco (Nicotiana tabacum L.) tissue were immunoassayed after partial purification by buffer-ether partition. Crown-gall tumor tissue, which is auxin-autotrophic, and pith tissue depleted of auxin by the diffusion method contained, respectively, 26.7 ng and <0.5 ng extractable IAA per gram fresh weight. More... »

PAGES

173-180

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00396195

DOI

http://dx.doi.org/10.1007/bf00396195

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029815373

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24420324


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/07", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Agricultural and Veterinary Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0703", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Crop and Pasture Production", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biology, Princeton University, 08540, Princeton, NJ, USA", 
          "id": "http://www.grid.ac/institutes/grid.16750.35", 
          "name": [
            "Department of Biology, Princeton University, 08540, Princeton, NJ, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pengelly", 
        "givenName": "William", 
        "id": "sg:person.01140025331.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140025331.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Botany, University of Illinois, 61801, Urbana, IL, USA", 
          "id": "http://www.grid.ac/institutes/grid.35403.31", 
          "name": [
            "Department of Botany, University of Illinois, 61801, Urbana, IL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Meins", 
        "givenName": "Frederick", 
        "id": "sg:person.01136614547.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136614547.28"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00385095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007132517", 
          "https://doi.org/10.1007/bf00385095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-88488-7_16", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005556672", 
          "https://doi.org/10.1007/978-3-642-88488-7_16"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00390855", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016864726", 
          "https://doi.org/10.1007/bf00390855"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1977-01", 
    "datePublishedReg": "1977-01-01", 
    "description": "We have developed a specific radioimmunoassay [RIA] for indole-3-acetic acid (IAA) in the 0.2 ng to 12 ng range which, in principle, can be extended to other indole auxins as well. Methods are presented for obtaining suitable antibody, for the RIA procedure, and for measuring IAA in methanolic extracts of plant tissues. Antibody specific for IAA was obtained from rabbits immunized with IAA bound to bovine serum albumin by formaldehyde treatment. In assays with this antibody, 2,4-dichlorophenoxyacetic acid and indoles structurally related to IAA reacted from 300- to 3000-fold less than did IAA itself. However, \u03b1-and \u03b2-naphthaleneacetic acid reacted significantly and hence interfered with the assay. Extracts of tobacco (Nicotiana tabacum L.) tissue were immunoassayed after partial purification by buffer-ether partition. Crown-gall tumor tissue, which is auxin-autotrophic, and pith tissue depleted of auxin by the diffusion method contained, respectively, 26.7 ng and <0.5 ng extractable IAA per gram fresh weight.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00396195", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "136"
      }
    ], 
    "keywords": [
      "suitable antibodies", 
      "serum albumin", 
      "indole auxins", 
      "tobacco tissues", 
      "diffusion method", 
      "RIA procedure", 
      "specific radioimmunoassay", 
      "indole-3-acetic acid", 
      "dichlorophenoxyacetic acid", 
      "assays", 
      "purification", 
      "plant tissues", 
      "acid", 
      "tumor tissue", 
      "antibodies", 
      "partial purification", 
      "radioimmunoassay", 
      "method", 
      "methanolic extract", 
      "tissue", 
      "albumin", 
      "range", 
      "extract", 
      "formaldehyde treatment", 
      "pith tissue", 
      "crown gall tumor tissue", 
      "naphthaleneacetic acid", 
      "rabbits", 
      "treatment", 
      "principles", 
      "weight", 
      "quantity", 
      "indole", 
      "procedure", 
      "partition", 
      "fresh weight", 
      "auxin"
    ], 
    "name": "A specific radioimmunoassay for nanogram quantities of the auxin, indole-3-acetic acid", 
    "pagination": "173-180", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1029815373"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00396195"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24420324"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00396195", 
      "https://app.dimensions.ai/details/publication/pub.1029815373"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_142.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00396195"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00396195'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00396195'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00396195'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00396195'


 

This table displays all metadata directly associated to this object as RDF triples.

128 TRIPLES      21 PREDICATES      68 URIs      55 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00396195 schema:about anzsrc-for:06
2 anzsrc-for:0607
3 anzsrc-for:07
4 anzsrc-for:0703
5 schema:author N104db9ea25d34570bb6a55433bfb807a
6 schema:citation sg:pub.10.1007/978-3-642-88488-7_16
7 sg:pub.10.1007/bf00385095
8 sg:pub.10.1007/bf00390855
9 schema:datePublished 1977-01
10 schema:datePublishedReg 1977-01-01
11 schema:description We have developed a specific radioimmunoassay [RIA] for indole-3-acetic acid (IAA) in the 0.2 ng to 12 ng range which, in principle, can be extended to other indole auxins as well. Methods are presented for obtaining suitable antibody, for the RIA procedure, and for measuring IAA in methanolic extracts of plant tissues. Antibody specific for IAA was obtained from rabbits immunized with IAA bound to bovine serum albumin by formaldehyde treatment. In assays with this antibody, 2,4-dichlorophenoxyacetic acid and indoles structurally related to IAA reacted from 300- to 3000-fold less than did IAA itself. However, α-and β-naphthaleneacetic acid reacted significantly and hence interfered with the assay. Extracts of tobacco (Nicotiana tabacum L.) tissue were immunoassayed after partial purification by buffer-ether partition. Crown-gall tumor tissue, which is auxin-autotrophic, and pith tissue depleted of auxin by the diffusion method contained, respectively, 26.7 ng and <0.5 ng extractable IAA per gram fresh weight.
12 schema:genre article
13 schema:isAccessibleForFree false
14 schema:isPartOf N56294db940ea4fc09d69feb754628c7b
15 Nb58659689af74e9cb328278433fbdaa0
16 sg:journal.1054035
17 schema:keywords RIA procedure
18 acid
19 albumin
20 antibodies
21 assays
22 auxin
23 crown gall tumor tissue
24 dichlorophenoxyacetic acid
25 diffusion method
26 extract
27 formaldehyde treatment
28 fresh weight
29 indole
30 indole auxins
31 indole-3-acetic acid
32 methanolic extract
33 method
34 naphthaleneacetic acid
35 partial purification
36 partition
37 pith tissue
38 plant tissues
39 principles
40 procedure
41 purification
42 quantity
43 rabbits
44 radioimmunoassay
45 range
46 serum albumin
47 specific radioimmunoassay
48 suitable antibodies
49 tissue
50 tobacco tissues
51 treatment
52 tumor tissue
53 weight
54 schema:name A specific radioimmunoassay for nanogram quantities of the auxin, indole-3-acetic acid
55 schema:pagination 173-180
56 schema:productId N6685628c9b754dbca10e14ff8c6baa16
57 Nbbc5bf1b93b7416b9b7077ad0e6aee36
58 Nfd93b451ca064ae9b61d4fbfb85536ad
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029815373
60 https://doi.org/10.1007/bf00396195
61 schema:sdDatePublished 2022-12-01T06:18
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher N0d741777ea944f9383b132507b86722a
64 schema:url https://doi.org/10.1007/bf00396195
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N0d741777ea944f9383b132507b86722a schema:name Springer Nature - SN SciGraph project
69 rdf:type schema:Organization
70 N104db9ea25d34570bb6a55433bfb807a rdf:first sg:person.01140025331.52
71 rdf:rest N76b78a2222c24e34b9dec460918d6968
72 N56294db940ea4fc09d69feb754628c7b schema:volumeNumber 136
73 rdf:type schema:PublicationVolume
74 N6685628c9b754dbca10e14ff8c6baa16 schema:name pubmed_id
75 schema:value 24420324
76 rdf:type schema:PropertyValue
77 N76b78a2222c24e34b9dec460918d6968 rdf:first sg:person.01136614547.28
78 rdf:rest rdf:nil
79 Nb58659689af74e9cb328278433fbdaa0 schema:issueNumber 2
80 rdf:type schema:PublicationIssue
81 Nbbc5bf1b93b7416b9b7077ad0e6aee36 schema:name doi
82 schema:value 10.1007/bf00396195
83 rdf:type schema:PropertyValue
84 Nfd93b451ca064ae9b61d4fbfb85536ad schema:name dimensions_id
85 schema:value pub.1029815373
86 rdf:type schema:PropertyValue
87 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
88 schema:name Biological Sciences
89 rdf:type schema:DefinedTerm
90 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
91 schema:name Plant Biology
92 rdf:type schema:DefinedTerm
93 anzsrc-for:07 schema:inDefinedTermSet anzsrc-for:
94 schema:name Agricultural and Veterinary Sciences
95 rdf:type schema:DefinedTerm
96 anzsrc-for:0703 schema:inDefinedTermSet anzsrc-for:
97 schema:name Crop and Pasture Production
98 rdf:type schema:DefinedTerm
99 sg:journal.1054035 schema:issn 0032-0935
100 1432-2048
101 schema:name Planta
102 schema:publisher Springer Nature
103 rdf:type schema:Periodical
104 sg:person.01136614547.28 schema:affiliation grid-institutes:grid.35403.31
105 schema:familyName Meins
106 schema:givenName Frederick
107 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136614547.28
108 rdf:type schema:Person
109 sg:person.01140025331.52 schema:affiliation grid-institutes:grid.16750.35
110 schema:familyName Pengelly
111 schema:givenName William
112 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140025331.52
113 rdf:type schema:Person
114 sg:pub.10.1007/978-3-642-88488-7_16 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005556672
115 https://doi.org/10.1007/978-3-642-88488-7_16
116 rdf:type schema:CreativeWork
117 sg:pub.10.1007/bf00385095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007132517
118 https://doi.org/10.1007/bf00385095
119 rdf:type schema:CreativeWork
120 sg:pub.10.1007/bf00390855 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016864726
121 https://doi.org/10.1007/bf00390855
122 rdf:type schema:CreativeWork
123 grid-institutes:grid.16750.35 schema:alternateName Department of Biology, Princeton University, 08540, Princeton, NJ, USA
124 schema:name Department of Biology, Princeton University, 08540, Princeton, NJ, USA
125 rdf:type schema:Organization
126 grid-institutes:grid.35403.31 schema:alternateName Department of Botany, University of Illinois, 61801, Urbana, IL, USA
127 schema:name Department of Botany, University of Illinois, 61801, Urbana, IL, USA
128 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...