Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-05

AUTHORS

C. L. Armstrong, C. E. Green

ABSTRACT

Friable, embryogenic maize (Zea mays L.), inbred line A188, callus was established and maintained for more than one year without apparent loss of friability or embryogenic potential. Embryoid development was abundant in these cultures and plants were easily regenerated. Frequencies of friable-callus initiation and somatic-embryoid formation increased linearly with addition to N6 medium (C.C. Chu et al. 1975, Sci. Sin. [Peking] 18, 659-668) of up to 25 mM L-proline. Proline additions up to 9 mM to MS medium (inorganic elements of T. Murashige and F. Skoog 1962, Physiol. Plant. 15, 473-497, plus 0.5 mg 1(-1) thiamine hydrochloride and 150 mg 1(-1) L-asparagine monohydrate) did not stimulate embryoid formation. A major part of the difference between MS and N6 media could be attributed to their respective inorganic nitrogen components. L-Glutamine was not a satisfactory substitute for L-proline. Of 111 regenerated plants grown to maturity from three independent friable, embryogenic cell lines ranging in age from three to seven months, only four plants were abnormal based on morphology and pollen sterility. Seed was produced by 77% of the regenerated plants. More... »

PAGES

207-214

Journal

TITLE

Planta

ISSUE

2

VOLUME

164

Author Affiliations

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  • Identifiers

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    http://scigraph.springernature.com/pub.10.1007/bf00396083

    DOI

    http://dx.doi.org/10.1007/bf00396083

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1029032788

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24249562


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