Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1983-02

AUTHORS

T. Boller, A. Gehri, F. Mauch, U. Vögeli

ABSTRACT

Ethylene induced an endochitinase in primary leaves of Phaseolus vulgaris L. The enzyme formed chitobiose and higher chitin oligosaccharides from insoluble, colloidal or regenerated chitin. Less than 5% of the total chitinolytic activity was detected in an exochitinase assay proposed by Abeles et al. (1970, Plant Physiol. 47, 129–134) for ethylene-induced chitinase. In ethylene-treated plants, chitinase activity started to increase after a lag of 6 h and was induced 30 fold within 24 h. Exogenously supplied ethylene at 1 nl ml−1 was sufficient for half-maximal induction, and enhancement of the endogenous ethylene formation also enhanced chitinase activity. Cycloheximide prevented the induction. Among various hydrolases tested, only chitinase and, to a lesser extent, β-1,3-glucanase were induced by ethylene. Induction of chitinase by ethylene occurred in many different plant species. Ethylene-induced chitinase was purified by affinity chromatography on a column of regenerated chitin. Its apparent molecular weight obtained by sodium dodecyl sulfate-gel electrophoresis was 30,000; the molecular weight determined from filtration through Sephadex G-75 was 22,000. The purified enzyme attacked chitin in isolated cell walls of Fusarium solani. It also acted as a lysozyme when incubated with Micrococcus lysodeikticus. It is concluded that ethylene-induced chitinase functions as a defense enzyme against fungal and bacterial invaders. More... »

PAGES

22-31

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00394536

DOI

http://dx.doi.org/10.1007/bf00394536

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024658349

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24263941


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Botanisches Institut der Universit\u00e4t, Sch\u00f6nbeinstrasse 6, CH-4056, Basel, Switzerland", 
          "id": "http://www.grid.ac/institutes/grid.6612.3", 
          "name": [
            "Botanisches Institut der Universit\u00e4t, Sch\u00f6nbeinstrasse 6, CH-4056, Basel, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Boller", 
        "givenName": "T.", 
        "id": "sg:person.013370042647.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013370042647.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Botanisches Institut der Universit\u00e4t, Sch\u00f6nbeinstrasse 6, CH-4056, Basel, Switzerland", 
          "id": "http://www.grid.ac/institutes/grid.6612.3", 
          "name": [
            "Botanisches Institut der Universit\u00e4t, Sch\u00f6nbeinstrasse 6, CH-4056, Basel, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gehri", 
        "givenName": "A.", 
        "id": "sg:person.07421774603.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07421774603.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Botanisches Institut der Universit\u00e4t, Sch\u00f6nbeinstrasse 6, CH-4056, Basel, Switzerland", 
          "id": "http://www.grid.ac/institutes/grid.6612.3", 
          "name": [
            "Botanisches Institut der Universit\u00e4t, Sch\u00f6nbeinstrasse 6, CH-4056, Basel, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mauch", 
        "givenName": "F.", 
        "id": "sg:person.01000021515.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01000021515.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Botanisches Institut der Universit\u00e4t, Sch\u00f6nbeinstrasse 6, CH-4056, Basel, Switzerland", 
          "id": "http://www.grid.ac/institutes/grid.6612.3", 
          "name": [
            "Botanisches Institut der Universit\u00e4t, Sch\u00f6nbeinstrasse 6, CH-4056, Basel, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "V\u00f6geli", 
        "givenName": "U.", 
        "id": "sg:person.01205645430.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205645430.24"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00384904", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009481692", 
          "https://doi.org/10.1007/bf00384904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00393286", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018757756", 
          "https://doi.org/10.1007/bf00393286"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1983-02", 
    "datePublishedReg": "1983-02-01", 
    "description": "Ethylene induced an endochitinase in primary leaves of Phaseolus vulgaris L. The enzyme formed chitobiose and higher chitin oligosaccharides from insoluble, colloidal or regenerated chitin. Less than 5% of the total chitinolytic activity was detected in an exochitinase assay proposed by Abeles et al. (1970, Plant Physiol. 47, 129\u2013134) for ethylene-induced chitinase. In ethylene-treated plants, chitinase activity started to increase after a lag of 6 h and was induced 30 fold within 24 h. Exogenously supplied ethylene at 1 nl ml\u22121 was sufficient for half-maximal induction, and enhancement of the endogenous ethylene formation also enhanced chitinase activity. Cycloheximide prevented the induction. Among various hydrolases tested, only chitinase and, to a lesser extent, \u03b2-1,3-glucanase were induced by ethylene. Induction of chitinase by ethylene occurred in many different plant species. Ethylene-induced chitinase was purified by affinity chromatography on a column of regenerated chitin. Its apparent molecular weight obtained by sodium dodecyl sulfate-gel electrophoresis was 30,000; the molecular weight determined from filtration through Sephadex G-75 was 22,000. The purified enzyme attacked chitin in isolated cell walls of Fusarium solani. It also acted as a lysozyme when incubated with Micrococcus lysodeikticus. It is concluded that ethylene-induced chitinase functions as a defense enzyme against fungal and bacterial invaders.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00394536", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "157"
      }
    ], 
    "keywords": [
      "chitinase activity", 
      "different plant species", 
      "ethylene-treated plants", 
      "Phaseolus vulgaris L.", 
      "total chitinolytic activity", 
      "induction of chitinase", 
      "sodium dodecyl sulfate gel electrophoresis", 
      "dodecyl sulfate gel electrophoresis", 
      "half-maximal induction", 
      "plant species", 
      "apparent molecular weight", 
      "endogenous ethylene formation", 
      "chitinase functions", 
      "sulfate gel electrophoresis", 
      "cell wall", 
      "primary leaves", 
      "chitinolytic activity", 
      "possible functions", 
      "bean leaves", 
      "defense enzymes", 
      "chitinase", 
      "chitin oligosaccharides", 
      "vulgaris L.", 
      "Fusarium solani", 
      "bacterial invaders", 
      "affinity chromatography", 
      "Micrococcus lysodeikticus", 
      "enzyme", 
      "molecular weight", 
      "leaves", 
      "chitin", 
      "induction", 
      "ethylene formation", 
      "invaders", 
      "endochitinase", 
      "glucanase", 
      "hydrolases", 
      "lesser extent", 
      "fungal", 
      "solani", 
      "species", 
      "plants", 
      "exochitinase", 
      "ethylene", 
      "Sephadex G", 
      "activity", 
      "cycloheximide", 
      "L.", 
      "chitobiose", 
      "electrophoresis", 
      "oligosaccharides", 
      "function", 
      "purification", 
      "folds", 
      "lysozyme", 
      "Abeles et al", 
      "regenerated chitin", 
      "formation", 
      "chromatography", 
      "weight", 
      "filtration", 
      "wall", 
      "extent", 
      "column", 
      "lag", 
      "et al", 
      "enhancement", 
      "properties", 
      "al"
    ], 
    "name": "Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function", 
    "pagination": "22-31", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1024658349"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00394536"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24263941"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00394536", 
      "https://app.dimensions.ai/details/publication/pub.1024658349"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_174.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00394536"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00394536'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00394536'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00394536'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00394536'


 

This table displays all metadata directly associated to this object as RDF triples.

163 TRIPLES      21 PREDICATES      98 URIs      87 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00394536 schema:about anzsrc-for:06
2 anzsrc-for:0607
3 schema:author N53eedbc10de84044b60d332f5981b2c9
4 schema:citation sg:pub.10.1007/bf00384904
5 sg:pub.10.1007/bf00393286
6 sg:pub.10.1038/227680a0
7 schema:datePublished 1983-02
8 schema:datePublishedReg 1983-02-01
9 schema:description Ethylene induced an endochitinase in primary leaves of Phaseolus vulgaris L. The enzyme formed chitobiose and higher chitin oligosaccharides from insoluble, colloidal or regenerated chitin. Less than 5% of the total chitinolytic activity was detected in an exochitinase assay proposed by Abeles et al. (1970, Plant Physiol. 47, 129–134) for ethylene-induced chitinase. In ethylene-treated plants, chitinase activity started to increase after a lag of 6 h and was induced 30 fold within 24 h. Exogenously supplied ethylene at 1 nl ml−1 was sufficient for half-maximal induction, and enhancement of the endogenous ethylene formation also enhanced chitinase activity. Cycloheximide prevented the induction. Among various hydrolases tested, only chitinase and, to a lesser extent, β-1,3-glucanase were induced by ethylene. Induction of chitinase by ethylene occurred in many different plant species. Ethylene-induced chitinase was purified by affinity chromatography on a column of regenerated chitin. Its apparent molecular weight obtained by sodium dodecyl sulfate-gel electrophoresis was 30,000; the molecular weight determined from filtration through Sephadex G-75 was 22,000. The purified enzyme attacked chitin in isolated cell walls of Fusarium solani. It also acted as a lysozyme when incubated with Micrococcus lysodeikticus. It is concluded that ethylene-induced chitinase functions as a defense enzyme against fungal and bacterial invaders.
10 schema:genre article
11 schema:isAccessibleForFree false
12 schema:isPartOf N95f3ae0cfd864cd79ded9abf65e82be1
13 Nda5e7db515044c4d9d674f6b8c5bc5a9
14 sg:journal.1054035
15 schema:keywords Abeles et al
16 Fusarium solani
17 L.
18 Micrococcus lysodeikticus
19 Phaseolus vulgaris L.
20 Sephadex G
21 activity
22 affinity chromatography
23 al
24 apparent molecular weight
25 bacterial invaders
26 bean leaves
27 cell wall
28 chitin
29 chitin oligosaccharides
30 chitinase
31 chitinase activity
32 chitinase functions
33 chitinolytic activity
34 chitobiose
35 chromatography
36 column
37 cycloheximide
38 defense enzymes
39 different plant species
40 dodecyl sulfate gel electrophoresis
41 electrophoresis
42 endochitinase
43 endogenous ethylene formation
44 enhancement
45 enzyme
46 et al
47 ethylene
48 ethylene formation
49 ethylene-treated plants
50 exochitinase
51 extent
52 filtration
53 folds
54 formation
55 function
56 fungal
57 glucanase
58 half-maximal induction
59 hydrolases
60 induction
61 induction of chitinase
62 invaders
63 lag
64 leaves
65 lesser extent
66 lysozyme
67 molecular weight
68 oligosaccharides
69 plant species
70 plants
71 possible functions
72 primary leaves
73 properties
74 purification
75 regenerated chitin
76 sodium dodecyl sulfate gel electrophoresis
77 solani
78 species
79 sulfate gel electrophoresis
80 total chitinolytic activity
81 vulgaris L.
82 wall
83 weight
84 schema:name Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function
85 schema:pagination 22-31
86 schema:productId N58faf5343b2549f8a8496fa90ae6da6e
87 N7152a0106b9c48468aca3e5417bf42b3
88 Na8bb4f78b9a94fddacf75b9fb0dd1214
89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024658349
90 https://doi.org/10.1007/bf00394536
91 schema:sdDatePublished 2022-10-01T06:27
92 schema:sdLicense https://scigraph.springernature.com/explorer/license/
93 schema:sdPublisher Nfa7347e6336c4a89ace6e4fdad34b383
94 schema:url https://doi.org/10.1007/bf00394536
95 sgo:license sg:explorer/license/
96 sgo:sdDataset articles
97 rdf:type schema:ScholarlyArticle
98 N13cdecdab5174bdd8695a81d2cc5b0cf rdf:first sg:person.01205645430.24
99 rdf:rest rdf:nil
100 N3f2de60d519049689bceebbcaebb5e9d rdf:first sg:person.01000021515.71
101 rdf:rest N13cdecdab5174bdd8695a81d2cc5b0cf
102 N53eedbc10de84044b60d332f5981b2c9 rdf:first sg:person.013370042647.07
103 rdf:rest Nc079a2093acd42bf83c6c2f4bdec62e1
104 N58faf5343b2549f8a8496fa90ae6da6e schema:name dimensions_id
105 schema:value pub.1024658349
106 rdf:type schema:PropertyValue
107 N7152a0106b9c48468aca3e5417bf42b3 schema:name pubmed_id
108 schema:value 24263941
109 rdf:type schema:PropertyValue
110 N95f3ae0cfd864cd79ded9abf65e82be1 schema:volumeNumber 157
111 rdf:type schema:PublicationVolume
112 Na8bb4f78b9a94fddacf75b9fb0dd1214 schema:name doi
113 schema:value 10.1007/bf00394536
114 rdf:type schema:PropertyValue
115 Nc079a2093acd42bf83c6c2f4bdec62e1 rdf:first sg:person.07421774603.24
116 rdf:rest N3f2de60d519049689bceebbcaebb5e9d
117 Nda5e7db515044c4d9d674f6b8c5bc5a9 schema:issueNumber 1
118 rdf:type schema:PublicationIssue
119 Nfa7347e6336c4a89ace6e4fdad34b383 schema:name Springer Nature - SN SciGraph project
120 rdf:type schema:Organization
121 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
122 schema:name Biological Sciences
123 rdf:type schema:DefinedTerm
124 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
125 schema:name Plant Biology
126 rdf:type schema:DefinedTerm
127 sg:journal.1054035 schema:issn 0032-0935
128 1432-2048
129 schema:name Planta
130 schema:publisher Springer Nature
131 rdf:type schema:Periodical
132 sg:person.01000021515.71 schema:affiliation grid-institutes:grid.6612.3
133 schema:familyName Mauch
134 schema:givenName F.
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01000021515.71
136 rdf:type schema:Person
137 sg:person.01205645430.24 schema:affiliation grid-institutes:grid.6612.3
138 schema:familyName Vögeli
139 schema:givenName U.
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205645430.24
141 rdf:type schema:Person
142 sg:person.013370042647.07 schema:affiliation grid-institutes:grid.6612.3
143 schema:familyName Boller
144 schema:givenName T.
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013370042647.07
146 rdf:type schema:Person
147 sg:person.07421774603.24 schema:affiliation grid-institutes:grid.6612.3
148 schema:familyName Gehri
149 schema:givenName A.
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07421774603.24
151 rdf:type schema:Person
152 sg:pub.10.1007/bf00384904 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009481692
153 https://doi.org/10.1007/bf00384904
154 rdf:type schema:CreativeWork
155 sg:pub.10.1007/bf00393286 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018757756
156 https://doi.org/10.1007/bf00393286
157 rdf:type schema:CreativeWork
158 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
159 https://doi.org/10.1038/227680a0
160 rdf:type schema:CreativeWork
161 grid-institutes:grid.6612.3 schema:alternateName Botanisches Institut der Universität, Schönbeinstrasse 6, CH-4056, Basel, Switzerland
162 schema:name Botanisches Institut der Universität, Schönbeinstrasse 6, CH-4056, Basel, Switzerland
163 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...