A new immunochemical method for the quantitative measurement of specific gene products in man-rodent somatic cell hybrids View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-01

AUTHORS

P. G. de Groot, M. N. Hamers, A. Westerveld, A. W. Schram, P. Meera Khan, J. M. Tager

ABSTRACT

An immunochemical method has been developed for the quantitative determination of species-specific gene products, for instance alpha-galactosidase and N-acetyl-alpha-galactosaminidase, in man-rodent hybrid cells and in the parental cell lines. Antisera raised against the purified enzymes are covalently coupled to Sepharose 4B. The gene products are specifically removed from a cell lysate by incubating with the appropriate Sepharose-coupled antiserum. After centrifugation followed by washing of the precipitated Sepharose, the enzymic activity can be quantitatively measured on the Sepharose beads. With this technique it has been demonstrated that the ability of human N-acetyl-alpha-galactosaminidase (also known as alpha-galactosidase B) to hydrolyze alpha-galactosidic linkages is lost when the enzyme is expressed in man-Chinese hamster hybrid cells. More... »

PAGES

295-304

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00394294

DOI

http://dx.doi.org/10.1007/bf00394294

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042815962

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/83282


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