Auxin binding to corn coleoptile membranes: Kinetics and specificity View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1976-01

AUTHORS

Susan Batt, Malcolm B. Wilkins, Michael A. Venis

ABSTRACT

Detailed examination of binding over the range 10(-7)-10(-6) M suggests that membrane preparations from coleoptiles of Zea mays L., cv Kelvedon 33 contain at least two sets of high affinity binding sites for 1-naphthylacetic acid (NAA), with dissociation constants of 1.8×10(-7) M (site 1) and 14.5×10(-7) M (site 2). Similar studies with 3-indolylacetic acid (IAA) also indicate two sets of binding sites, whose concentrations are closely comparable to those deduced for NAA. A substantial proportion of the total binding activity is retained in a detergent-solubilized preparation. Using [(14)C]NAA the interactions of a range of analogues with each of the binding sites have been examined with the aid of double reciprocal plots. The specificity of site 2 is compatible with that expected for an auxin receptor, in that only active auxins, antiauxin transport inhibitors are able to compete with [(14)C]NAA for the binding sites. Site 1 on the other hand is less specific, since it appears to bind all compounds tested, including physiologically inactive analogues. More... »

PAGES

7-13

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00390838

DOI

http://dx.doi.org/10.1007/bf00390838

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010404543

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24424536


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