The estimation of phenylalanine ammonia-lyase (PAL) activity in intact cells of higher plant tissue View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1981-07

AUTHORS

Heike Holländer, Nikolaus Amrhein

ABSTRACT

A previously described procedure for the estimation of relative activities of phenylalanine ammonia-lyase (EC 4.3.1.5) in intact plant cells (Amrhein et al. (1976) Planta 131, 33–40) was reexamined for its specificity and its applicability to various tissues. In buckwheat hypocotyl segments 3H is stereospecifically released from the pro-3S-position of L-[2,3-3H]phenylalanine and is thus due to phenylalanine ammonia-lyase activity. In buck wheat and sunflower leaf disks, however, 3H release occurs from both the 2- and 3-positions of the labeled substrate and can only partially be attributed to phenylalanine ammonia-lyase activity. More... »

PAGES

374-378

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00388265

DOI

http://dx.doi.org/10.1007/bf00388265

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016700096

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24301035


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