Dormancy in somatic embryos and seeds ofVitis: changes in endogenous abscisic acid during embryogeny and germination View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1982-03

AUTHORS

K. Rajasekaran, J. Vine, M. G. Mullins

ABSTRACT

Abscisic acid (ABA) in extracts of somatic embryos and seeds of Gloryvine (Vitis vinifera L.xV. rupestris Scheele) was measured by gas chromatography-mass spectrometry-selected ion monitoring using deuterated ABA, (±)-[C-3Me-(2)H3]ABA, ([(2)H3]ABA) as internal standard. The ABA content increased rapidly during embryogeny (0.035 ng/embryo at the globular stage to 0.22 ng/embryo at the mature stage). The level of ABA in the tissues of somatic embryos, expressed in ng/mg dry weight, decreased from the globular stage (0.76 ng/mg) to the mature stage (0.25 ng/mg). Chilling (4° C) induced normal germination of seeds and mature somatic embryos and precocious germination of globular, heart-shaped and torpedoshaped somatic embryos. In all cases chilling led to a marked reduction in endogenous ABA. Exogenous (±)-ABA inhibited the germination of chilled somatic embryos. More... »

PAGES

139-144

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00387907

DOI

http://dx.doi.org/10.1007/bf00387907

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011067715

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24275974


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