In-ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1977-01

AUTHORS

James McD. Stewart, Cecilia Lee Hsu

ABSTRACT

A convenient and reliable method for culturing cotton embryos is needed to obtain interspecific hybrids of this genus. C.A. Beasley and I.P. Ting (Amer. J. Bot. 60, 130, 1973) developed a phytohormone-supplemented medium (BTP) upon which the growth of ovules was similar that of in situ ovules. This medium was examined for in-ovulo embryo culture. Although good ovule growth occurred on BTP no embryos developed to maturity. However, when the medium was supplemented with NH4+, more than 50% of the ovules produced mature embryos, and many of these germinated precociously after 8–10 weeks of culture. After germination seedlings were established on a separate medium designed to give balanced root and shoot growth. Subsequently young plants could be transferred to pots for greenhouse culture. More... »

PAGES

113-117

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00387547

DOI

http://dx.doi.org/10.1007/bf00387547

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036228182

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24420627


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