The estimation of phenylalanine ammonia-lyase (PAL)-activity in intact cells of higher plant tissue View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1976-01

AUTHORS

Nikolaus Amrhein, Karl-Heinz Gödeke, Jürgen Gerhardt

ABSTRACT

A procedure is described which permits the estimation of the relative activity of phenylalanine ammonia-lyase (E.C. 4.3.1.5.) in intact plant cells, exemplified by buckwheat hypocotyls. Hypocotyl segments are incubated at pH 5.5 with L-[3-3H]phenylalanine. N3HH2, which is liberated from phenylalanine by the action of phenylalanine ammonia-lyase, equilibrates with tissue water to yield 3HOH, which is recovered by sublimation. Participation of phenylalanine transaminase in the reactions leading to 3HOH formation is excluded, and it is conclusively shown that 3HOH is formed intracellularly and not by enzymatic activity leaking out of wounded tissue. More... »

PAGES

33-40

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00387342

DOI

http://dx.doi.org/10.1007/bf00387342

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043478375

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24424692


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