Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-05

AUTHORS

Jung-Hoe Kim, Wei-Shou Hu

ABSTRACT

Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density. More... »

PAGES

135-140

References to SciGraph publications

  • 1986-03. Hydrodynamic effects on cells in agitated tissue culture reactors in BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00386146

    DOI

    http://dx.doi.org/10.1007/bf00386146

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1006268711

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22358626


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Korea Advanced Institute of Science and Technology", 
              "id": "https://www.grid.ac/institutes/grid.37172.30", 
              "name": [
                "Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Seoul, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Jung-Hoe", 
            "id": "sg:person.01300137557.41", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300137557.41"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Minnesota", 
              "id": "https://www.grid.ac/institutes/grid.17635.36", 
              "name": [
                "Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hu", 
            "givenName": "Wei-Shou", 
            "id": "sg:person.01064261433.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064261433.49"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00369462", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001627156", 
              "https://doi.org/10.1007/bf00369462"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/b978-0-408-02732-8.50027-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003631158"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1749-6632.1981.tb14176.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009092778"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.260290917", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022202342"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.260300412", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024376708"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.260290117", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026653950"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1989-05", 
        "datePublishedReg": "1989-05-01", 
        "description": "Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00386146", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1086307", 
            "issn": [
              "1381-5741", 
              "1573-0603"
            ], 
            "name": "Cytotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "2"
          }
        ], 
        "name": "Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation", 
        "pagination": "135-140", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "1ddc3d531f256ccfb2846774e23aa4d9c9926bc56447632a7d3361e359f25fb8"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "22358626"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "8807027"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00386146"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1006268711"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00386146", 
          "https://app.dimensions.ai/details/publication/pub.1006268711"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:47", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130792_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00386146"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00386146'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00386146'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00386146'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00386146'


     

    This table displays all metadata directly associated to this object as RDF triples.

    98 TRIPLES      21 PREDICATES      35 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00386146 schema:about anzsrc-for:06
    2 anzsrc-for:0601
    3 schema:author N27f7a703e30e4302bd2b64e5df40e11a
    4 schema:citation sg:pub.10.1007/bf00369462
    5 https://doi.org/10.1002/bit.260290117
    6 https://doi.org/10.1002/bit.260290917
    7 https://doi.org/10.1002/bit.260300412
    8 https://doi.org/10.1016/b978-0-408-02732-8.50027-4
    9 https://doi.org/10.1111/j.1749-6632.1981.tb14176.x
    10 schema:datePublished 1989-05
    11 schema:datePublishedReg 1989-05-01
    12 schema:description Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density.
    13 schema:genre research_article
    14 schema:inLanguage en
    15 schema:isAccessibleForFree false
    16 schema:isPartOf N968fcb15bb9a446b8038a63e0de2bb53
    17 Nc1232a2872bc4c55bd983866c1fe16d6
    18 sg:journal.1086307
    19 schema:name Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation
    20 schema:pagination 135-140
    21 schema:productId N33604cf2eb8048fb90f5af5d1338eca7
    22 Na491511051364941865bf5d41f82bc1e
    23 Nc72d3337784d46d9a4eb91e695d81822
    24 Nd14b7149dd8d4f029d45e035b231802b
    25 Nf660f746be17466e94c401bbf8fde7f2
    26 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006268711
    27 https://doi.org/10.1007/bf00386146
    28 schema:sdDatePublished 2019-04-11T13:47
    29 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    30 schema:sdPublisher Nc2fc5fe161f44dc89acf57ddee2c1fae
    31 schema:url http://link.springer.com/10.1007/BF00386146
    32 sgo:license sg:explorer/license/
    33 sgo:sdDataset articles
    34 rdf:type schema:ScholarlyArticle
    35 N27f7a703e30e4302bd2b64e5df40e11a rdf:first sg:person.01300137557.41
    36 rdf:rest N6304d8be25e24f1d8adae28c987eb266
    37 N33604cf2eb8048fb90f5af5d1338eca7 schema:name nlm_unique_id
    38 schema:value 8807027
    39 rdf:type schema:PropertyValue
    40 N6304d8be25e24f1d8adae28c987eb266 rdf:first sg:person.01064261433.49
    41 rdf:rest rdf:nil
    42 N968fcb15bb9a446b8038a63e0de2bb53 schema:volumeNumber 2
    43 rdf:type schema:PublicationVolume
    44 Na491511051364941865bf5d41f82bc1e schema:name dimensions_id
    45 schema:value pub.1006268711
    46 rdf:type schema:PropertyValue
    47 Nc1232a2872bc4c55bd983866c1fe16d6 schema:issueNumber 2
    48 rdf:type schema:PublicationIssue
    49 Nc2fc5fe161f44dc89acf57ddee2c1fae schema:name Springer Nature - SN SciGraph project
    50 rdf:type schema:Organization
    51 Nc72d3337784d46d9a4eb91e695d81822 schema:name pubmed_id
    52 schema:value 22358626
    53 rdf:type schema:PropertyValue
    54 Nd14b7149dd8d4f029d45e035b231802b schema:name doi
    55 schema:value 10.1007/bf00386146
    56 rdf:type schema:PropertyValue
    57 Nf660f746be17466e94c401bbf8fde7f2 schema:name readcube_id
    58 schema:value 1ddc3d531f256ccfb2846774e23aa4d9c9926bc56447632a7d3361e359f25fb8
    59 rdf:type schema:PropertyValue
    60 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    61 schema:name Biological Sciences
    62 rdf:type schema:DefinedTerm
    63 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    64 schema:name Biochemistry and Cell Biology
    65 rdf:type schema:DefinedTerm
    66 sg:journal.1086307 schema:issn 1381-5741
    67 1573-0603
    68 schema:name Cytotechnology
    69 rdf:type schema:Periodical
    70 sg:person.01064261433.49 schema:affiliation https://www.grid.ac/institutes/grid.17635.36
    71 schema:familyName Hu
    72 schema:givenName Wei-Shou
    73 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064261433.49
    74 rdf:type schema:Person
    75 sg:person.01300137557.41 schema:affiliation https://www.grid.ac/institutes/grid.37172.30
    76 schema:familyName Kim
    77 schema:givenName Jung-Hoe
    78 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300137557.41
    79 rdf:type schema:Person
    80 sg:pub.10.1007/bf00369462 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001627156
    81 https://doi.org/10.1007/bf00369462
    82 rdf:type schema:CreativeWork
    83 https://doi.org/10.1002/bit.260290117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026653950
    84 rdf:type schema:CreativeWork
    85 https://doi.org/10.1002/bit.260290917 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022202342
    86 rdf:type schema:CreativeWork
    87 https://doi.org/10.1002/bit.260300412 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024376708
    88 rdf:type schema:CreativeWork
    89 https://doi.org/10.1016/b978-0-408-02732-8.50027-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003631158
    90 rdf:type schema:CreativeWork
    91 https://doi.org/10.1111/j.1749-6632.1981.tb14176.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009092778
    92 rdf:type schema:CreativeWork
    93 https://www.grid.ac/institutes/grid.17635.36 schema:alternateName University of Minnesota
    94 schema:name Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, USA
    95 rdf:type schema:Organization
    96 https://www.grid.ac/institutes/grid.37172.30 schema:alternateName Korea Advanced Institute of Science and Technology
    97 schema:name Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Seoul, Korea
    98 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...