In vitro germination and pollen tube growth of maize (Zea mays L.) pollen View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1973-09

AUTHORS

P. L. Pfahler, H. F. Linskens

ABSTRACT

Pollen grains from two hybrids, WF9xH55 (W) and K64xK55 (K) were collected and a sample from each was cultured immediately (0 h). The remainder was subdivided and stored at 2, 20, and 35° C. At 3, 6, 12, 24, 36, 48, 72, 96, and 120 h, a sample was cultured. The culture medium contained 15% sucrose, 0.6% bacto-agar, 0.03% calcium nitrate and 0.01% boric acid. Storage at 2° C resulted in a large increase in germination percentage in both W and K reaching a maximum at 24 h and then slowly decreasing with additional storage. No germination was observed at 96 h with W and at 120 h with K. The complete loss of germination occurred during a 24 h period and was very abrupt. At 20° C, a similar but less pronounced pattern was observed. However, after 24 h, aggregates of 50–1 000 pollen grains developed during storage in both W and K. Storage of W at 35° C slightly decreased the germination percentage at 3 h and eliminated it at 6 h. Storage of K at 35° C substantially increased the germination percentage at 3 h with further increases in storage periods resulting in the aggregation of grains. This general pattern of an increase at shorter storage periods followed by a gradual decrease as the storage period was extended, was found for pollen tube length and growth rate. In vitro germination characteristics can be substantially altered by the temperature and length of storage and the response to storage is associated with pollen source. More... »

PAGES

253-259

References to SciGraph publications

  • 1961-07. Physiology of pollen in THE BOTANICAL REVIEW
  • 1972-01. In vitro germination and pollen tube growth of maize (Zea mays L.) pollen in THEORETICAL AND APPLIED GENETICS
  • 1968-07. Development, physiology, and biochemistry of corn and wheat pollen in THE BOTANICAL REVIEW
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00385108

    DOI

    http://dx.doi.org/10.1007/bf00385108

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1042010762

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24469577


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