Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1970-12

AUTHORS

Toshiyuki Nagata, Itaru Takebe

ABSTRACT

Protoplasts were isolated from palisade tissue of tobacco leaves by treatment with pectinase and cellulase under aseptic conditions, and were cultured in a synthetic liquid medium. Calcofluor, a fluorescent brightener, was found to be an excellent stain for plant cell walls and was used to demonstrate regeneration of cell walls in these protoplasts. The cultured protoplasts regenerated cell walls by the 3rd day of culture, giving rise to spherical cells. The majority of the protoplasts regenerating cell walls underwent mitosis and cell division. The cycle of mitosis and cell division was repeated 2-3 times during 2 weeks of culture. Some of the nutritional conditions affecting division in the cultured protoplasts were studied. More... »

PAGES

301-308

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00385097

DOI

http://dx.doi.org/10.1007/bf00385097

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028757873

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24500300


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