The specificity of carrier-mediated auxin transport by suspension-cultured crown gall cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1977-01

AUTHORS

P. H. Rubery

ABSTRACT

1. The specificity of the auxin transport system of suspension-cultured crown gall cells from Parthenocissus tricuspidata Planch- is examined with regard to 2,4-Dichlorophenoxyacetic acid (2,4 D), l-Naphthylacetic acid (NAA) and Benzoic acid (BA) as well as for indole-3-acetic acid (IAA). - 2. All four weak acids can be accumulated by the cells from a medium more acidic than the cytoplasm. This is by virtue of non-specific passive diffusion of their lipid-soluble protonated forms down a concentration gradient. The corresponding anionic species are much less permeant. The extent of the accumulation is dependent on the pH difference that is maintained by the cells between their cytoplasm and the incubation medium. Studies of the concentration dependence of BA and NAA net uptake at a series of external pHs suggest that an acidification of the cytoplasm can be eventually brought about by the entry of weak acid into the cells. - 3. The uptake of 2,4 D, as well as that of IAA, has a saturable carrier-mediated component in addition to the passive diffusion of the undissociated acid. These saturable components probably represent anion uptake and appear to be mediated by a common carrier. The kinetic studies provided no evidence for the participation of carriers in the transport of BA or NAA. - 4. It was shown that the efflux of 2,4 D also has a carrier-mediated component and it is suggested that both the influx and efflux of IAA and 2,4 D occur on a common carrier. - 5. The inhibitor of polar auxin transport, 2,3,5-triiodobenzoic acid (TIBA), stimulates the net uptake of IAA by inhibiting carrier-mediated efflux of IAA from the cells. However, TIBA could not be demonstrated to have a significant effect on 2,4 D transport and any perturbation that occurs is very small in comparison with its effect on IAA movement. To account for this, the proposed common carrier could exhibit some difference in its internal binding characteristics betweend 2,4 D and IAA. An alternative explanation is that a second carrier is present, which mediates IAA efflux only, and which is inhibited by TIBA. - 6. TIBA has no significant effect on the transport of either BA or NAA, except to bring about an inhibition of net uptake, and a corresponding stimulation of efflux, when it is present at concentrations sufficient to acidify the cytoplasm. -7. The crown gall cells are compared to intact plant tissues capable of polar auxin transport with regard to the specificities exhibited for the transport of the auxins IAA, 2,4 D and NAA and the non-auxin BA. More... »

PAGES

275-283

Journal

TITLE

Planta

ISSUE

3

VOLUME

135

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00384900

DOI

http://dx.doi.org/10.1007/bf00384900

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007138876

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24420094


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Cambridge", 
          "id": "https://www.grid.ac/institutes/grid.5335.0", 
          "name": [
            "Department of Biochemistry, Cambridge University, Tennis Court Road, CB2 1QW, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rubery", 
        "givenName": "P. H.", 
        "id": "sg:person.01016011655.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016011655.46"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf01305716", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004286149", 
          "https://doi.org/10.1007/bf01305716"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01305716", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004286149", 
          "https://doi.org/10.1007/bf01305716"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj0550170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004553857"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj0550170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004553857"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00390838", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010404543", 
          "https://doi.org/10.1007/bf00390838"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00390838", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010404543", 
          "https://doi.org/10.1007/bf00390838"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4165(72)90308-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014093465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4165(72)90308-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014093465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00390285", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015618542", 
          "https://doi.org/10.1007/bf00390285"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00390285", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015618542", 
          "https://doi.org/10.1007/bf00390285"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00389401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030371953", 
          "https://doi.org/10.1007/bf00389401"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00389401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030371953", 
          "https://doi.org/10.1007/bf00389401"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244285a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030435410", 
          "https://doi.org/10.1038/newbio244285a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244285a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030435410", 
          "https://doi.org/10.1038/newbio244285a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244285a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030435410", 
          "https://doi.org/10.1038/newbio244285a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.53.3.464", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031750202"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1469-8137.1963.tb06308.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033749112"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00388387", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040645604", 
          "https://doi.org/10.1007/bf00388387"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00388387", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040645604", 
          "https://doi.org/10.1007/bf00388387"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1469-8137.1975.tb02602.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048835868"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080297625", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1977-01", 
    "datePublishedReg": "1977-01-01", 
    "description": "1. The specificity of the auxin transport system of suspension-cultured crown gall cells from Parthenocissus tricuspidata Planch- is examined with regard to 2,4-Dichlorophenoxyacetic acid (2,4 D), l-Naphthylacetic acid (NAA) and Benzoic acid (BA) as well as for indole-3-acetic acid (IAA). - 2. All four weak acids can be accumulated by the cells from a medium more acidic than the cytoplasm. This is by virtue of non-specific passive diffusion of their lipid-soluble protonated forms down a concentration gradient. The corresponding anionic species are much less permeant. The extent of the accumulation is dependent on the pH difference that is maintained by the cells between their cytoplasm and the incubation medium. Studies of the concentration dependence of BA and NAA net uptake at a series of external pHs suggest that an acidification of the cytoplasm can be eventually brought about by the entry of weak acid into the cells. - 3. The uptake of 2,4 D, as well as that of IAA, has a saturable carrier-mediated component in addition to the passive diffusion of the undissociated acid. These saturable components probably represent anion uptake and appear to be mediated by a common carrier. The kinetic studies provided no evidence for the participation of carriers in the transport of BA or NAA. - 4. It was shown that the efflux of 2,4 D also has a carrier-mediated component and it is suggested that both the influx and efflux of IAA and 2,4 D occur on a common carrier. - 5. The inhibitor of polar auxin transport, 2,3,5-triiodobenzoic acid (TIBA), stimulates the net uptake of IAA by inhibiting carrier-mediated efflux of IAA from the cells. However, TIBA could not be demonstrated to have a significant effect on 2,4 D transport and any perturbation that occurs is very small in comparison with its effect on IAA movement. To account for this, the proposed common carrier could exhibit some difference in its internal binding characteristics betweend 2,4 D and IAA. An alternative explanation is that a second carrier is present, which mediates IAA efflux only, and which is inhibited by TIBA. - 6. TIBA has no significant effect on the transport of either BA or NAA, except to bring about an inhibition of net uptake, and a corresponding stimulation of efflux, when it is present at concentrations sufficient to acidify the cytoplasm. -7. The crown gall cells are compared to intact plant tissues capable of polar auxin transport with regard to the specificities exhibited for the transport of the auxins IAA, 2,4 D and NAA and the non-auxin BA. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00384900", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "135"
      }
    ], 
    "name": "The specificity of carrier-mediated auxin transport by suspension-cultured crown gall cells", 
    "pagination": "275-283", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "220185c9deeea091d0b318f0eacabd35f1f3fc94b33a7d43e92f47c3ce39aa41"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24420094"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "1250576"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00384900"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1007138876"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00384900", 
      "https://app.dimensions.ai/details/publication/pub.1007138876"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:47", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130792_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00384900"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00384900'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00384900'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00384900'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00384900'


 

This table displays all metadata directly associated to this object as RDF triples.

110 TRIPLES      21 PREDICATES      41 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00384900 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Nb8abfb6a011941c4aa18d73854aaeebc
4 schema:citation sg:pub.10.1007/bf00388387
5 sg:pub.10.1007/bf00389401
6 sg:pub.10.1007/bf00390285
7 sg:pub.10.1007/bf00390838
8 sg:pub.10.1007/bf01305716
9 sg:pub.10.1038/newbio244285a0
10 https://app.dimensions.ai/details/publication/pub.1080297625
11 https://doi.org/10.1016/0304-4165(72)90308-x
12 https://doi.org/10.1042/bj0550170
13 https://doi.org/10.1104/pp.53.3.464
14 https://doi.org/10.1111/j.1469-8137.1963.tb06308.x
15 https://doi.org/10.1111/j.1469-8137.1975.tb02602.x
16 schema:datePublished 1977-01
17 schema:datePublishedReg 1977-01-01
18 schema:description 1. The specificity of the auxin transport system of suspension-cultured crown gall cells from Parthenocissus tricuspidata Planch- is examined with regard to 2,4-Dichlorophenoxyacetic acid (2,4 D), l-Naphthylacetic acid (NAA) and Benzoic acid (BA) as well as for indole-3-acetic acid (IAA). - 2. All four weak acids can be accumulated by the cells from a medium more acidic than the cytoplasm. This is by virtue of non-specific passive diffusion of their lipid-soluble protonated forms down a concentration gradient. The corresponding anionic species are much less permeant. The extent of the accumulation is dependent on the pH difference that is maintained by the cells between their cytoplasm and the incubation medium. Studies of the concentration dependence of BA and NAA net uptake at a series of external pHs suggest that an acidification of the cytoplasm can be eventually brought about by the entry of weak acid into the cells. - 3. The uptake of 2,4 D, as well as that of IAA, has a saturable carrier-mediated component in addition to the passive diffusion of the undissociated acid. These saturable components probably represent anion uptake and appear to be mediated by a common carrier. The kinetic studies provided no evidence for the participation of carriers in the transport of BA or NAA. - 4. It was shown that the efflux of 2,4 D also has a carrier-mediated component and it is suggested that both the influx and efflux of IAA and 2,4 D occur on a common carrier. - 5. The inhibitor of polar auxin transport, 2,3,5-triiodobenzoic acid (TIBA), stimulates the net uptake of IAA by inhibiting carrier-mediated efflux of IAA from the cells. However, TIBA could not be demonstrated to have a significant effect on 2,4 D transport and any perturbation that occurs is very small in comparison with its effect on IAA movement. To account for this, the proposed common carrier could exhibit some difference in its internal binding characteristics betweend 2,4 D and IAA. An alternative explanation is that a second carrier is present, which mediates IAA efflux only, and which is inhibited by TIBA. - 6. TIBA has no significant effect on the transport of either BA or NAA, except to bring about an inhibition of net uptake, and a corresponding stimulation of efflux, when it is present at concentrations sufficient to acidify the cytoplasm. -7. The crown gall cells are compared to intact plant tissues capable of polar auxin transport with regard to the specificities exhibited for the transport of the auxins IAA, 2,4 D and NAA and the non-auxin BA.
19 schema:genre research_article
20 schema:inLanguage en
21 schema:isAccessibleForFree false
22 schema:isPartOf N2ce5cbd8bf89473ea41f72754dd54839
23 N42f0b291af7c4b1986214988681e0cff
24 sg:journal.1054035
25 schema:name The specificity of carrier-mediated auxin transport by suspension-cultured crown gall cells
26 schema:pagination 275-283
27 schema:productId N19f6716853d04518a9411b36ffe84053
28 N9a9c97b2d1b7492f9f545a6d3a5c8170
29 Nb7d55a78d80245e3ba068f5d07601368
30 Nd45a4930afc84527a8f4e13000e410ca
31 Ndae8ed1dd8d94448b43758f4f437f223
32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007138876
33 https://doi.org/10.1007/bf00384900
34 schema:sdDatePublished 2019-04-11T13:47
35 schema:sdLicense https://scigraph.springernature.com/explorer/license/
36 schema:sdPublisher Nee3fd62c024041c5980253556cb78130
37 schema:url http://link.springer.com/10.1007/BF00384900
38 sgo:license sg:explorer/license/
39 sgo:sdDataset articles
40 rdf:type schema:ScholarlyArticle
41 N19f6716853d04518a9411b36ffe84053 schema:name readcube_id
42 schema:value 220185c9deeea091d0b318f0eacabd35f1f3fc94b33a7d43e92f47c3ce39aa41
43 rdf:type schema:PropertyValue
44 N2ce5cbd8bf89473ea41f72754dd54839 schema:issueNumber 3
45 rdf:type schema:PublicationIssue
46 N42f0b291af7c4b1986214988681e0cff schema:volumeNumber 135
47 rdf:type schema:PublicationVolume
48 N9a9c97b2d1b7492f9f545a6d3a5c8170 schema:name nlm_unique_id
49 schema:value 1250576
50 rdf:type schema:PropertyValue
51 Nb7d55a78d80245e3ba068f5d07601368 schema:name dimensions_id
52 schema:value pub.1007138876
53 rdf:type schema:PropertyValue
54 Nb8abfb6a011941c4aa18d73854aaeebc rdf:first sg:person.01016011655.46
55 rdf:rest rdf:nil
56 Nd45a4930afc84527a8f4e13000e410ca schema:name doi
57 schema:value 10.1007/bf00384900
58 rdf:type schema:PropertyValue
59 Ndae8ed1dd8d94448b43758f4f437f223 schema:name pubmed_id
60 schema:value 24420094
61 rdf:type schema:PropertyValue
62 Nee3fd62c024041c5980253556cb78130 schema:name Springer Nature - SN SciGraph project
63 rdf:type schema:Organization
64 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
65 schema:name Biological Sciences
66 rdf:type schema:DefinedTerm
67 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
68 schema:name Biochemistry and Cell Biology
69 rdf:type schema:DefinedTerm
70 sg:journal.1054035 schema:issn 0032-0935
71 1432-2048
72 schema:name Planta
73 rdf:type schema:Periodical
74 sg:person.01016011655.46 schema:affiliation https://www.grid.ac/institutes/grid.5335.0
75 schema:familyName Rubery
76 schema:givenName P. H.
77 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016011655.46
78 rdf:type schema:Person
79 sg:pub.10.1007/bf00388387 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040645604
80 https://doi.org/10.1007/bf00388387
81 rdf:type schema:CreativeWork
82 sg:pub.10.1007/bf00389401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030371953
83 https://doi.org/10.1007/bf00389401
84 rdf:type schema:CreativeWork
85 sg:pub.10.1007/bf00390285 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015618542
86 https://doi.org/10.1007/bf00390285
87 rdf:type schema:CreativeWork
88 sg:pub.10.1007/bf00390838 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010404543
89 https://doi.org/10.1007/bf00390838
90 rdf:type schema:CreativeWork
91 sg:pub.10.1007/bf01305716 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004286149
92 https://doi.org/10.1007/bf01305716
93 rdf:type schema:CreativeWork
94 sg:pub.10.1038/newbio244285a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030435410
95 https://doi.org/10.1038/newbio244285a0
96 rdf:type schema:CreativeWork
97 https://app.dimensions.ai/details/publication/pub.1080297625 schema:CreativeWork
98 https://doi.org/10.1016/0304-4165(72)90308-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014093465
99 rdf:type schema:CreativeWork
100 https://doi.org/10.1042/bj0550170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004553857
101 rdf:type schema:CreativeWork
102 https://doi.org/10.1104/pp.53.3.464 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031750202
103 rdf:type schema:CreativeWork
104 https://doi.org/10.1111/j.1469-8137.1963.tb06308.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1033749112
105 rdf:type schema:CreativeWork
106 https://doi.org/10.1111/j.1469-8137.1975.tb02602.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1048835868
107 rdf:type schema:CreativeWork
108 https://www.grid.ac/institutes/grid.5335.0 schema:alternateName University of Cambridge
109 schema:name Department of Biochemistry, Cambridge University, Tennis Court Road, CB2 1QW, Cambridge, UK
110 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...