Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1975-01

AUTHORS

K. N. Kao, M. R. Michayluk

ABSTRACT

When Vicia hajastana Grossh. cells or protoplasts were cultured at a high population density (ca. 5000 cells or protoplasts/ml), they were able to grow in a mineral-salt solution supplemented with sucrose (or glucose), a few vitamins, and 2,4-dichlorophenoxyacetic acid. They were not able to survive when cultured at a low population density unless the medium was supplemented with zeatin, naphthalene-1-acetic acid, nucleic-acid bases, amino acids, other sugars, sugar alcohols, and organic acids. Vicia cells were able to grow at an initial population density of 25-50 cells/ml in this defined medium. The population density could be lowered to 1-2 cells/ml with good growth when the mineral-salt medium was enriched with organic acids, sugars, sugar alcohols, coconut water, and casamino acids. The protoplasts also grew best in a medium enriched with these supplements. Three individual protoplasts were isolated and each one was cultured in a separate dish containing 4 ml of this medium. Within 30-40 days, each one had grown indefinitely and formed a mass of cells (ca. 10(7)). More... »

PAGES

105-110

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00380613

DOI

http://dx.doi.org/10.1007/bf00380613

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006514198

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24430152


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