The droplet technique: measurement of calcium extrusion from single isolated mammalian cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-10

AUTHORS

A. V. Tepikin, J. Llopis, V. A. Snitsarev, D. V. Gallacher, O. H. Petersen

ABSTRACT

This paper contains a description of the modified droplet technique that is designed to monitor Ca2+ extrusion from single isolated pancreatic acinar cells. A cell loaded with calcium indicator is maintained in a small droplet of solution containing another calcium indicator. Differences in the optical properties of the intracellular and extracellular indicators allows one to monitor simultaneously intracellular and extracellular calcium concentrations. The paper contains a description of the calibration procedure that is used to calculate intracellular and extracellular calcium concentrations. The advantages and disadvantages of different pairs of extracellular and intracellular indicators are discussed. More... »

PAGES

664-670

References to SciGraph publications

  • 1991-07. Extrusion of calcium from a single isolated neuron of the snailHelix pomatia in THE JOURNAL OF MEMBRANE BIOLOGY
  • 1993-09. Evidence for agonist-induced export of intracellular Ca2+ in epithelial cells in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00374591

    DOI

    http://dx.doi.org/10.1007/bf00374591

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7838690


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    151 schema:name Medical Research Council Secretory Control Research Group, The Physiologiclal Laboratory, The University of Liverpool, P.O. Box 147, L69 3BX, Liverpool
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