Ca2+ release in cultured rat epididymal cells during hypoosmotic swelling View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-10

AUTHORS

A. Y. H. Leung, P. Y. D. Wong

ABSTRACT

Microfluorimetric studies were carried out to investigate the effects of hypoosmotic swelling on intracellular Ca2+ concentration ([Ca2+]i) in single rat epididymal cells. In Ca2+-free solution containing 50 μmol/l ethylenebis(oxonitrilo)tetraacetate (EGTA) hypoosmotic swelling (−160 mosmol/l) induced a transient rise in [Ca2+]i which was either monophasic, biphasic or oscillatory. The [Ca2+]i responses to repeated hypoosmotic stimulations followed a decremental pattern. However, if 2.5 mmol/l Ca2+ was admitted during the recovery period between successive stimulations, the second and the third [Ca2+]i responses were slightly greater than the first. Increasing the change in osmolarity from −14±1.0 to −154±1.5 mosmol/l increased the rise in [Ca2+]i but reduced the [Ca2+]i response to subsequent ionomycin stimulation (4 μmol/l). The swelling- and the ionomycin-induced rises in [Ca2+]i followed a reciprocal pattern. It was suggested that intracellular Ca2+ release in response to cell swelling in the epididymal epithelium might play a role in cell volume regulation and the control of epididymal fluid osmolarity. More... »

PAGES

77-81

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00374506

DOI

http://dx.doi.org/10.1007/bf00374506

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037973551

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8272386


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