Strategies for optimising serum-free media View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-01

AUTHORS

Guy Hewlett

ABSTRACT

Two of the main reasons for developing serum-free medium are to improve the growth and to increase the productivity of a cell. This review has attetmpted to show that these goals can be achieved far more easily than generally realised by utilising the methods that have been developed during the past decade and by using a limited number of standard supplements. Serum-free media have contributed greatly to the advances made in recent years in the fields of cell biology and mammalian cell technology and it is clear that they will continue to play a major role in the clarification of cellular regulatory processes and in the refinement of processes in modern cyto-technology. More... »

PAGES

3-14

References to SciGraph publications

  • 1988-02. Polyethylene glycols for promoting the growth of mammalian cells in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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  • 1988-11. Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using balb/c 3T3 mouse embryo fibroblasts in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 1988-12. Scaleup of Insect Cell Cultures: Protective Effects of Pluronic F-68 in NATURE BIOTECHNOLOGY
  • 1981. Survival and Growth Requirements of Nontransformed Cells in TISSUE GROWTH FACTORS
  • 1990-01. Serial cultivation of suspended BHK 21/13 cells in serum-reduced and serum-free medium supplemented with various membrane protective agents in CYTOTECHNOLOGY
  • 1989-07. Low protein serum-free medium for antibody-production in stirred bioreactors in BIOTECHNOLOGY LETTERS
  • 1983-12. Development of protein-deficient serum-free culture media for adherent cell lines suited for large scale cultivation in CYTOTECHNOLOGY
  • 1990-01. What should be focused in the study of cell culture technology for production of bioactive proteins in CYTOTECHNOLOGY
  • 1988. The use of plasma protein fractions as medium supplements for animal cell culture in BIOPROCESSES INCLUDING ANIMAL CELL CULTURE
  • 1988-02. Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free medium in CYTOTECHNOLOGY
  • 1988-11. Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors in CYTOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00365530

    DOI

    http://dx.doi.org/10.1007/bf00365530

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1004813096

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1367049


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