Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-11

AUTHORS

Volker Jäger, Jürgen Lehmann, Peter Friedl

ABSTRACT

A serum free medium was developed, that could be used for the large scale propagation of various cell lines in bioreactors. The medium is based on a 1:1 mixture of Iscove's Modified Dulbecco's Medium and Ham's Medium F12, supplemented with transferrin, insulin and a BSA/oleic acid complex. Several myelomas, hybridomas derived from different myelomas and spleen cells, and other lymphoid and non-lymphoid cell lines were cultivated at growth rates comparable to those observed using serum-supplemented media. There was furthermore no reduction in the formation of products such as monoclonal antibodies or recombinant human interleukin-2. More... »

PAGES

319-329

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00365077

DOI

http://dx.doi.org/10.1007/bf00365077

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050697816

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22359167


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