Non-uniform distribution of methylatable CCGG sequences on human chromosomes as shown by in situ methylation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-03

AUTHORS

Carlos Sentís, Paloma Ludeña, Jose Fernandez-Piqueras

ABSTRACT

We carried out in situ methylation of human chromosomes with the HpaII methylase using [3H]methyl-S-adenosyl-L-methionine as the methyl group carrier. Autoradiographs localising [3H]methyl groups show methylatable CCGG sequences in the R-bands as well as in the short arms of the acrocentric chromosomes that include ribosomal DNA. The strongest labelling was observed over a subset of R-bands, including T-bands. Since methylatable CCGG sequences are representative of the unmethylated fraction of DNA, we suggest that differences in the degree of DNA methylation could be involved in the structure and function of chromosomal bands. More... »

PAGES

267-271

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00352400

DOI

http://dx.doi.org/10.1007/bf00352400

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004467951

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8486078


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