Characterisation of repeated sequences from microdissected B chromosomes of Crepis capillaris View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-11

AUTHORS

M. Jamilena, M. Garrido-Ramos, M. Ruiz Rejón, C. Ruiz Rejón, J. S. Parker

ABSTRACT

The B chromosome of Crepis capillaris was isolated from the standard chromosomes by microdissection, and the chromosomal DNA amplified using the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). The PCR product was cloned and a B-specific library created and characterised. Southern and in situ hybridisation analyses of the DOP-PCR product from microdissected B chromosomes confirmed that the B chromosome is composed mainly of sequences also present in the A chromosomes but lacks the main repeated DNA families located in the A-chromosomal heterochromatin. From 100 clones analysed, 12% of the generated B-chromosomal library was shown to be composed of dispersed repeats located in both the A and B chromosomes. No B-specific repeated sequence was detected. One of the most abundant repeated DNAs within the library, the family B134, was further characterised. Repeating units show a sequence similarity range from 69% to 90% and are characterised by their richness in (CA)n repeats. In situ hybridisation revealed that members of this family are dispersed throughout the A and B chromosomes but are more concentrated in the pericentromeric heterochromatin of the B, indicating that the molecular organization of B heterochromatin is different from that of the A chromosomes. Compared with the A chromosomes, the Bs contain about 20,000 copies per micron more of the B134 sequence. This indicates that B134 was amplified on the B chromosome after its origin. The B134 sequences in the B chromosomes have also diverged from those on the A chromosomes. Although the DNA composition of A and B chromosomes is similar, Bs are evolving separately from A chromosomes at the molecular level. More... »

PAGES

113-120

References to SciGraph publications

  • 1995-02. A species specific satellite DNA family of Drosophila subsilvestris appearing predominantly in B chromosomes in CHROMOSOMA
  • 1994-04. Junctions between repetitive DNAs on the PSR chromosome of Nasonia vitripennis: Association of palindromes with recombination in JOURNAL OF MOLECULAR EVOLUTION
  • 1991-02. Isolation of a sequence common to A- and B-chromosomes of rye (Secale cereale) by microcloning in PLANT MOLECULAR BIOLOGY REPORTER
  • 1994-03. Utility of DNA amplified by degenerate oligonucleotide-primed PCR (DOP-PCR) from the total genome and defined chromosomal regions of field bean in MOLECULAR GENETICS AND GENOMICS
  • 1994-03. Chromosome 2-specific DNA clones from flow-sorted chromosomes of tomato in MOLECULAR GENETICS AND GENOMICS
  • 1990-06. The population cytogenetics of Crepis capillaris. III. B-chromosome effects on meiosis in HEREDITY
  • 1992-08. Molecular characterization of repetitive DNA sequences from a B chromosome in CHROMOSOMA
  • 1994-11. Molecular relationship between the A and B chromosomes of Crepis capillaris in HEREDITY
  • 1986-07. Population cytogenetics of Atractomorpha similis in CHROMOSOMA
  • 1993-03. Repetitive DNA sequence families in Crepis capillaris in CHROMOSOMA
  • 1989-02. Ribosomal RNA genes in B chromosomes of Crepis capillaris detected by non-radioactive in situ hybridization in HEREDITY
  • 1981-02. The distribution of repetitive DNAs between regular and supernumerary chromosomes in Species of Glossina (Tsetse): a two-step process in the origin of supernumeraries in CHROMOSOMA
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00347693

    DOI

    http://dx.doi.org/10.1007/bf00347693

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011788496

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8585988


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Blotting, Southern", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromosomes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cloning, Molecular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Primers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Plant", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Heterochromatin", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "In Situ Hybridization, Fluorescence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Micromanipulation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Repetitive Sequences, Nucleic Acid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Homology, Nucleic Acid", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Reading", 
              "id": "https://www.grid.ac/institutes/grid.9435.b", 
              "name": [
                "Department of Botany, School of Plant Sciences, University of Reading, RG6 2AS, Reading, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jamilena", 
            "givenName": "M.", 
            "id": "sg:person.0744031366.07", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744031366.07"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Granada", 
              "id": "https://www.grid.ac/institutes/grid.4489.1", 
              "name": [
                "Departmento de Genetica, Facultad de Ciencias, Universidad de Granada, E-18071, Granada, Spain"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Garrido-Ramos", 
            "givenName": "M.", 
            "id": "sg:person.01231076370.59", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231076370.59"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Granada", 
              "id": "https://www.grid.ac/institutes/grid.4489.1", 
              "name": [
                "Departmento de Genetica, Facultad de Ciencias, Universidad de Granada, E-18071, Granada, Spain"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rej\u00f3n", 
            "givenName": "M. Ruiz", 
            "id": "sg:person.014776061426.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014776061426.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Granada", 
              "id": "https://www.grid.ac/institutes/grid.4489.1", 
              "name": [
                "Departmento de Genetica, Facultad de Ciencias, Universidad de Granada, E-18071, Granada, Spain"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rej\u00f3n", 
            "givenName": "C. Ruiz", 
            "id": "sg:person.010753223441.71", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010753223441.71"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Reading", 
              "id": "https://www.grid.ac/institutes/grid.9435.b", 
              "name": [
                "Department of Botany, School of Plant Sciences, University of Reading, RG6 2AS, Reading, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Parker", 
            "givenName": "J. S.", 
            "id": "sg:person.07736350643.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07736350643.29"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00352401", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002298429", 
              "https://doi.org/10.1007/bf00352401"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00352401", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002298429", 
              "https://doi.org/10.1007/bf00352401"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00293529", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004403423", 
              "https://doi.org/10.1007/bf00293529"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00293529", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004403423", 
              "https://doi.org/10.1007/bf00293529"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02669286", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004408186", 
              "https://doi.org/10.1007/bf02669286"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02669286", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004408186", 
              "https://doi.org/10.1007/bf02669286"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.6.11.3934", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008922553"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1266/jjg.67.233", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010148000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1266/jjg.67.233", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010148000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1266/jjg.67.233", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010148000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1159/000133689", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011753436"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/hdy.1994.151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012552856", 
              "https://doi.org/10.1038/hdy.1994.151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/hdy.1994.151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012552856", 
              "https://doi.org/10.1038/hdy.1994.151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.85.2.529", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017771345"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1139/g93-095", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018806335"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/hdy.1990.47", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022570237", 
              "https://doi.org/10.1038/hdy.1990.47"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/hdy.1990.47", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022570237", 
              "https://doi.org/10.1038/hdy.1990.47"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0888-7543(92)90147-k", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024097503"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.74.12.5463", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025360556"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.79.21.6465", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033186583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1139/g90-137", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037690987"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00285278", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037850877", 
              "https://doi.org/10.1007/bf00285278"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00285278", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037850877", 
              "https://doi.org/10.1007/bf00285278"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00660317", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039798016", 
              "https://doi.org/10.1007/bf00660317"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00660317", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039798016", 
              "https://doi.org/10.1007/bf00660317"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/hdy.1989.8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041266434", 
              "https://doi.org/10.1038/hdy.1989.8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/hdy.1989.8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041266434", 
              "https://doi.org/10.1038/hdy.1989.8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00163152", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042338576", 
              "https://doi.org/10.1007/bf00163152"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00163152", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042338576", 
              "https://doi.org/10.1007/bf00163152"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00329579", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043078825", 
              "https://doi.org/10.1007/bf00329579"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00329579", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043078825", 
              "https://doi.org/10.1007/bf00329579"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.78.7.4490", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043490517"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00355318", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044026311", 
              "https://doi.org/10.1007/bf00355318"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00355318", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044026311", 
              "https://doi.org/10.1007/bf00355318"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1989.tb08402.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078987262"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082605315", 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00280314", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1082687868", 
              "https://doi.org/10.1007/bf00280314"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082719148", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1995-11", 
        "datePublishedReg": "1995-11-01", 
        "description": "The B chromosome of Crepis capillaris was isolated from the standard chromosomes by microdissection, and the chromosomal DNA amplified using the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). The PCR product was cloned and a B-specific library created and characterised. Southern and in situ hybridisation analyses of the DOP-PCR product from microdissected B chromosomes confirmed that the B chromosome is composed mainly of sequences also present in the A chromosomes but lacks the main repeated DNA families located in the A-chromosomal heterochromatin. From 100 clones analysed, 12% of the generated B-chromosomal library was shown to be composed of dispersed repeats located in both the A and B chromosomes. No B-specific repeated sequence was detected. One of the most abundant repeated DNAs within the library, the family B134, was further characterised. Repeating units show a sequence similarity range from 69% to 90% and are characterised by their richness in (CA)n repeats. In situ hybridisation revealed that members of this family are dispersed throughout the A and B chromosomes but are more concentrated in the pericentromeric heterochromatin of the B, indicating that the molecular organization of B heterochromatin is different from that of the A chromosomes. Compared with the A chromosomes, the Bs contain about 20,000 copies per micron more of the B134 sequence. This indicates that B134 was amplified on the B chromosome after its origin. The B134 sequences in the B chromosomes have also diverged from those on the A chromosomes. Although the DNA composition of A and B chromosomes is similar, Bs are evolving separately from A chromosomes at the molecular level.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00347693", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1312277", 
            "issn": [
              "0009-5915", 
              "1432-0886"
            ], 
            "name": "Chromosoma", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "104"
          }
        ], 
        "name": "Characterisation of repeated sequences from microdissected B chromosomes of Crepis capillaris", 
        "pagination": "113-120", 
        "productId": [
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00347693"
            ]
          }, 
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "382abf1f32373442a6a0049bb719b468061f9af22004cc21b08584aeae1bddd8"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1011788496"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "2985138R"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "8585988"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00347693", 
          "https://app.dimensions.ai/details/publication/pub.1011788496"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-15T08:52", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119747_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00347693"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00347693'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00347693'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00347693'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00347693'


     

    This table displays all metadata directly associated to this object as RDF triples.

    241 TRIPLES      21 PREDICATES      68 URIs      35 LITERALS      23 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00347693 schema:about N0c54cc377f3e4dab9d8fb92365e83faf
    2 N1cc33f0306c24aedb139408b903481ac
    3 N2a07dde064de4b72a804b9059747adab
    4 N2ebc07296c5e4f32851057402086a651
    5 N420e4063d4ac4d6a9f5c4748251b27bc
    6 N5b09c97ebc9c45f7a87d3720abbd383f
    7 N5f897204fdcd4fd280e1d4c5ff6021ca
    8 N8dd85958633b40768ab0cc5dbb669aae
    9 N91f50c7d24404b8b984140c872a5ba41
    10 N95948ff2411d4fa8ba68195e0928d1f2
    11 Nb16638ba62624b47bb1d8115164bc7f0
    12 Nb392c66f878b4df1a87d4d77d25d9fac
    13 Nb3fdc268142744049a9d0e6f05bee099
    14 Nc269f516d7b5439d90384cfdc2770e84
    15 anzsrc-for:06
    16 anzsrc-for:0604
    17 schema:author N850f7dbd2c844199baf61ba7299ef3fd
    18 schema:citation sg:pub.10.1007/bf00163152
    19 sg:pub.10.1007/bf00280314
    20 sg:pub.10.1007/bf00285278
    21 sg:pub.10.1007/bf00293529
    22 sg:pub.10.1007/bf00329579
    23 sg:pub.10.1007/bf00352401
    24 sg:pub.10.1007/bf00355318
    25 sg:pub.10.1007/bf00660317
    26 sg:pub.10.1007/bf02669286
    27 sg:pub.10.1038/hdy.1989.8
    28 sg:pub.10.1038/hdy.1990.47
    29 sg:pub.10.1038/hdy.1994.151
    30 https://app.dimensions.ai/details/publication/pub.1082605315
    31 https://app.dimensions.ai/details/publication/pub.1082719148
    32 https://doi.org/10.1002/j.1460-2075.1989.tb08402.x
    33 https://doi.org/10.1016/0888-7543(92)90147-k
    34 https://doi.org/10.1073/pnas.74.12.5463
    35 https://doi.org/10.1073/pnas.78.7.4490
    36 https://doi.org/10.1073/pnas.79.21.6465
    37 https://doi.org/10.1073/pnas.85.2.529
    38 https://doi.org/10.1128/mcb.6.11.3934
    39 https://doi.org/10.1139/g90-137
    40 https://doi.org/10.1139/g93-095
    41 https://doi.org/10.1159/000133689
    42 https://doi.org/10.1266/jjg.67.233
    43 schema:datePublished 1995-11
    44 schema:datePublishedReg 1995-11-01
    45 schema:description The B chromosome of Crepis capillaris was isolated from the standard chromosomes by microdissection, and the chromosomal DNA amplified using the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). The PCR product was cloned and a B-specific library created and characterised. Southern and in situ hybridisation analyses of the DOP-PCR product from microdissected B chromosomes confirmed that the B chromosome is composed mainly of sequences also present in the A chromosomes but lacks the main repeated DNA families located in the A-chromosomal heterochromatin. From 100 clones analysed, 12% of the generated B-chromosomal library was shown to be composed of dispersed repeats located in both the A and B chromosomes. No B-specific repeated sequence was detected. One of the most abundant repeated DNAs within the library, the family B134, was further characterised. Repeating units show a sequence similarity range from 69% to 90% and are characterised by their richness in (CA)n repeats. In situ hybridisation revealed that members of this family are dispersed throughout the A and B chromosomes but are more concentrated in the pericentromeric heterochromatin of the B, indicating that the molecular organization of B heterochromatin is different from that of the A chromosomes. Compared with the A chromosomes, the Bs contain about 20,000 copies per micron more of the B134 sequence. This indicates that B134 was amplified on the B chromosome after its origin. The B134 sequences in the B chromosomes have also diverged from those on the A chromosomes. Although the DNA composition of A and B chromosomes is similar, Bs are evolving separately from A chromosomes at the molecular level.
    46 schema:genre research_article
    47 schema:inLanguage en
    48 schema:isAccessibleForFree false
    49 schema:isPartOf N2ea554ec52ba4500b8b62eaee1c7ce2b
    50 N9f22fdfdcb2d4aad8c5847afa8e59d3d
    51 sg:journal.1312277
    52 schema:name Characterisation of repeated sequences from microdissected B chromosomes of Crepis capillaris
    53 schema:pagination 113-120
    54 schema:productId N3c0a71ace4c34ababc79378b026a6108
    55 N3d4869bba7e749dda3d838ef148e91f0
    56 N9c6bf1817729476983e162372bec5aae
    57 Nbaac25ac101a42228123da924f5e6412
    58 Nd436f8120e6749faaf495ee21f656293
    59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011788496
    60 https://doi.org/10.1007/bf00347693
    61 schema:sdDatePublished 2019-04-15T08:52
    62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    63 schema:sdPublisher Na579499e13c74fb18a992593b4e56eba
    64 schema:url http://link.springer.com/10.1007/BF00347693
    65 sgo:license sg:explorer/license/
    66 sgo:sdDataset articles
    67 rdf:type schema:ScholarlyArticle
    68 N0c54cc377f3e4dab9d8fb92365e83faf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    69 schema:name Chromosomes
    70 rdf:type schema:DefinedTerm
    71 N1cc33f0306c24aedb139408b903481ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    72 schema:name Heterochromatin
    73 rdf:type schema:DefinedTerm
    74 N2a07dde064de4b72a804b9059747adab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    75 schema:name DNA, Plant
    76 rdf:type schema:DefinedTerm
    77 N2ea554ec52ba4500b8b62eaee1c7ce2b schema:issueNumber 2
    78 rdf:type schema:PublicationIssue
    79 N2ebc07296c5e4f32851057402086a651 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    80 schema:name Micromanipulation
    81 rdf:type schema:DefinedTerm
    82 N3c0a71ace4c34ababc79378b026a6108 schema:name dimensions_id
    83 schema:value pub.1011788496
    84 rdf:type schema:PropertyValue
    85 N3d4869bba7e749dda3d838ef148e91f0 schema:name doi
    86 schema:value 10.1007/bf00347693
    87 rdf:type schema:PropertyValue
    88 N420e4063d4ac4d6a9f5c4748251b27bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Blotting, Southern
    90 rdf:type schema:DefinedTerm
    91 N50446bb3a6b540f5a5d6d6d62203b1a1 rdf:first sg:person.01231076370.59
    92 rdf:rest Ncaed643ef5e64e05a3482afe74f5ff16
    93 N5b09c97ebc9c45f7a87d3720abbd383f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name In Situ Hybridization, Fluorescence
    95 rdf:type schema:DefinedTerm
    96 N5f897204fdcd4fd280e1d4c5ff6021ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Repetitive Sequences, Nucleic Acid
    98 rdf:type schema:DefinedTerm
    99 N69e49550e90a406cb664f79be7950c01 rdf:first sg:person.07736350643.29
    100 rdf:rest rdf:nil
    101 N850f7dbd2c844199baf61ba7299ef3fd rdf:first sg:person.0744031366.07
    102 rdf:rest N50446bb3a6b540f5a5d6d6d62203b1a1
    103 N8dd85958633b40768ab0cc5dbb669aae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Polymerase Chain Reaction
    105 rdf:type schema:DefinedTerm
    106 N91f50c7d24404b8b984140c872a5ba41 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name DNA Primers
    108 rdf:type schema:DefinedTerm
    109 N95948ff2411d4fa8ba68195e0928d1f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Plants
    111 rdf:type schema:DefinedTerm
    112 N9c6bf1817729476983e162372bec5aae schema:name readcube_id
    113 schema:value 382abf1f32373442a6a0049bb719b468061f9af22004cc21b08584aeae1bddd8
    114 rdf:type schema:PropertyValue
    115 N9f22fdfdcb2d4aad8c5847afa8e59d3d schema:volumeNumber 104
    116 rdf:type schema:PublicationVolume
    117 Na579499e13c74fb18a992593b4e56eba schema:name Springer Nature - SN SciGraph project
    118 rdf:type schema:Organization
    119 Nb16638ba62624b47bb1d8115164bc7f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Base Sequence
    121 rdf:type schema:DefinedTerm
    122 Nb392c66f878b4df1a87d4d77d25d9fac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Molecular Sequence Data
    124 rdf:type schema:DefinedTerm
    125 Nb3fdc268142744049a9d0e6f05bee099 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Sequence Homology, Nucleic Acid
    127 rdf:type schema:DefinedTerm
    128 Nbaac25ac101a42228123da924f5e6412 schema:name nlm_unique_id
    129 schema:value 2985138R
    130 rdf:type schema:PropertyValue
    131 Nc269f516d7b5439d90384cfdc2770e84 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Cloning, Molecular
    133 rdf:type schema:DefinedTerm
    134 Ncaed643ef5e64e05a3482afe74f5ff16 rdf:first sg:person.014776061426.19
    135 rdf:rest Ne1cd7ae1476c4cfb99890a072dd866fd
    136 Nd436f8120e6749faaf495ee21f656293 schema:name pubmed_id
    137 schema:value 8585988
    138 rdf:type schema:PropertyValue
    139 Ne1cd7ae1476c4cfb99890a072dd866fd rdf:first sg:person.010753223441.71
    140 rdf:rest N69e49550e90a406cb664f79be7950c01
    141 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    142 schema:name Biological Sciences
    143 rdf:type schema:DefinedTerm
    144 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    145 schema:name Genetics
    146 rdf:type schema:DefinedTerm
    147 sg:journal.1312277 schema:issn 0009-5915
    148 1432-0886
    149 schema:name Chromosoma
    150 rdf:type schema:Periodical
    151 sg:person.010753223441.71 schema:affiliation https://www.grid.ac/institutes/grid.4489.1
    152 schema:familyName Rejón
    153 schema:givenName C. Ruiz
    154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010753223441.71
    155 rdf:type schema:Person
    156 sg:person.01231076370.59 schema:affiliation https://www.grid.ac/institutes/grid.4489.1
    157 schema:familyName Garrido-Ramos
    158 schema:givenName M.
    159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231076370.59
    160 rdf:type schema:Person
    161 sg:person.014776061426.19 schema:affiliation https://www.grid.ac/institutes/grid.4489.1
    162 schema:familyName Rejón
    163 schema:givenName M. Ruiz
    164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014776061426.19
    165 rdf:type schema:Person
    166 sg:person.0744031366.07 schema:affiliation https://www.grid.ac/institutes/grid.9435.b
    167 schema:familyName Jamilena
    168 schema:givenName M.
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744031366.07
    170 rdf:type schema:Person
    171 sg:person.07736350643.29 schema:affiliation https://www.grid.ac/institutes/grid.9435.b
    172 schema:familyName Parker
    173 schema:givenName J. S.
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07736350643.29
    175 rdf:type schema:Person
    176 sg:pub.10.1007/bf00163152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042338576
    177 https://doi.org/10.1007/bf00163152
    178 rdf:type schema:CreativeWork
    179 sg:pub.10.1007/bf00280314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082687868
    180 https://doi.org/10.1007/bf00280314
    181 rdf:type schema:CreativeWork
    182 sg:pub.10.1007/bf00285278 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037850877
    183 https://doi.org/10.1007/bf00285278
    184 rdf:type schema:CreativeWork
    185 sg:pub.10.1007/bf00293529 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004403423
    186 https://doi.org/10.1007/bf00293529
    187 rdf:type schema:CreativeWork
    188 sg:pub.10.1007/bf00329579 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043078825
    189 https://doi.org/10.1007/bf00329579
    190 rdf:type schema:CreativeWork
    191 sg:pub.10.1007/bf00352401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002298429
    192 https://doi.org/10.1007/bf00352401
    193 rdf:type schema:CreativeWork
    194 sg:pub.10.1007/bf00355318 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044026311
    195 https://doi.org/10.1007/bf00355318
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1007/bf00660317 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039798016
    198 https://doi.org/10.1007/bf00660317
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1007/bf02669286 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004408186
    201 https://doi.org/10.1007/bf02669286
    202 rdf:type schema:CreativeWork
    203 sg:pub.10.1038/hdy.1989.8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041266434
    204 https://doi.org/10.1038/hdy.1989.8
    205 rdf:type schema:CreativeWork
    206 sg:pub.10.1038/hdy.1990.47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022570237
    207 https://doi.org/10.1038/hdy.1990.47
    208 rdf:type schema:CreativeWork
    209 sg:pub.10.1038/hdy.1994.151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012552856
    210 https://doi.org/10.1038/hdy.1994.151
    211 rdf:type schema:CreativeWork
    212 https://app.dimensions.ai/details/publication/pub.1082605315 schema:CreativeWork
    213 https://app.dimensions.ai/details/publication/pub.1082719148 schema:CreativeWork
    214 https://doi.org/10.1002/j.1460-2075.1989.tb08402.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078987262
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1016/0888-7543(92)90147-k schema:sameAs https://app.dimensions.ai/details/publication/pub.1024097503
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1073/pnas.74.12.5463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025360556
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1073/pnas.78.7.4490 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043490517
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1073/pnas.79.21.6465 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033186583
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1073/pnas.85.2.529 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017771345
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1128/mcb.6.11.3934 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008922553
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1139/g90-137 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037690987
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1139/g93-095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018806335
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1159/000133689 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011753436
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1266/jjg.67.233 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010148000
    235 rdf:type schema:CreativeWork
    236 https://www.grid.ac/institutes/grid.4489.1 schema:alternateName University of Granada
    237 schema:name Departmento de Genetica, Facultad de Ciencias, Universidad de Granada, E-18071, Granada, Spain
    238 rdf:type schema:Organization
    239 https://www.grid.ac/institutes/grid.9435.b schema:alternateName University of Reading
    240 schema:name Department of Botany, School of Plant Sciences, University of Reading, RG6 2AS, Reading, UK
    241 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...