Ontology type: schema:ScholarlyArticle
1988-09
AUTHORS ABSTRACTWe have previously isolated and characterized a gene (rbcS-8B) from the wild-type species Nicotiana plumbaginifolia, encoding the small subunit of ribulose-1,5-bis-phosphate carboxylase. Using transgenic N. plumbaginifolia as a host, we found that a 5′ upstream region (-1038 to +32) of rbcS-8B contains all the sequences required for organ-specific and light-dependent expression of the gene. Here we report a detailed analysis of the 5′ upstream region of rbcS-8B. Gene transfer experiments indicate that the region between-1038 to-102 contains at least two enhancer-like elements. The proximal element located between-312 and-102, confers organ-specific and light-inducible expression upon a reporter gene and closely resembles previously identified elements in other light-responsive plant genes. The distal element with novel characteristics is located between-1038 and-589. It can enhance expression from the cauliflower mosaic virus 35S promoter, but only when the-90 to-46 region of the 35S promoter is present. It confers on the heterologous promoter the organ-specificity of a typical rbcS gene but the enhanced transcription in leaves is insensitive to light. These experiments show for the first time that organ-specificity and light-responsiveness can be determined by separable cis-regulatory elements. The implications for the regulation of light-responsive genes are discussed. More... »
PAGES16-23
http://scigraph.springernature.com/pub.10.1007/bf00340173
DOIhttp://dx.doi.org/10.1007/bf00340173
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/3226423
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