Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-01

AUTHORS

Patrick Charnay, Anne Louise, Alexandre Fritsch, David Perrin, Pierre Tiollais

ABSTRACT

Bacteriophage vectors derived from λplac5 have been constructed. Their genomes have one EcoRI restriction site which is located at the very beginning of the lac Z gene. The major part of this gene was deleted by an in vivo intramolecular recombination. These vectors allow the fusion of a gene or an operon with the beginning of the lac Z gene, placing them under the control of the lac promoter, which carries the UV5 mutation. Some of these vectors (λY) also include the lac Y gene and it too is under the control of the lac promoter. The λYEQS, which carries the Qam73 and Sam7 mutations, as well as safety mutations, has been certified as a B2 (EK2) vector by the French control commission “recombinaison génétique in vitro”. More... »

PAGES

171-178

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00337793

DOI

http://dx.doi.org/10.1007/bf00337793

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021960318

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/107393


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