Ontology type: schema:ScholarlyArticle
1979-01
AUTHORSPatrick Charnay, Anne Louise, Alexandre Fritsch, David Perrin, Pierre Tiollais
ABSTRACTBacteriophage vectors derived from λplac5 have been constructed. Their genomes have one EcoRI restriction site which is located at the very beginning of the lac Z gene. The major part of this gene was deleted by an in vivo intramolecular recombination. These vectors allow the fusion of a gene or an operon with the beginning of the lac Z gene, placing them under the control of the lac promoter, which carries the UV5 mutation. Some of these vectors (λY) also include the lac Y gene and it too is under the control of the lac promoter. The λYEQS, which carries the Qam73 and Sam7 mutations, as well as safety mutations, has been certified as a B2 (EK2) vector by the French control commission “recombinaison génétique in vitro”. More... »
PAGES171-178
http://scigraph.springernature.com/pub.10.1007/bf00337793
DOIhttp://dx.doi.org/10.1007/bf00337793
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/107393
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