Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-01

AUTHORS

Christine Pourcel, Christian Marchal, Anne Louise, Alexandre Fritsch, Pierre Tiollais

ABSTRACT

Bacteriophage lambda vectors, derived from λplac5 were constructed. Their genomes have only one EcoRI restriction site, located near the end of the β-galactosidase gene. Recombinants, constructed in vitro, having a DNA fragment inserted in the EcoRI site, are lac- and can be easily recognized. Expression of such foreign genes is then under the control of the lac promoter. Mutations Qam73 and Sam7 greatly increase the amount of β-galactosidase synthesized by the vector bacteriophage. The λZEQS vector has been certified B2 (EK2) by the French control commission “Recombinaisons génétiques in vitro”. More... »

PAGES

161-169

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00337792

DOI

http://dx.doi.org/10.1007/bf00337792

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020444176

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/107392


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