Heterologous transformation of Mucor circinelloides with the Phycomyces blakesleeanus leul gene View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-03

AUTHORS

Enrique A. Iturriaga, José M. Díaz-Mínguez, Ernesto P. Benito, Maria Isabel Alvarez, Arturo P. Eslava

ABSTRACT

The leu1 gene of Phycomyces blakesleeanus was isolated within a HindIII-HindIII genomic DNA fragment by heterologous hybridization screening of a cosmid library, making use of the Mucor circinelloides leuA gene as a probe. The complete nucleotide sequence of this fragment reveals a single 2070 bp ORF with no introns, which presents at least 68% homology with that of the leuA gene. The P. blakesleeanus leu1 gene has also been expressed in the M. circinelloides mutant R7B (leu-), which was used to isolate the leuA gene by complementation. The homology with other known sequences shows that the leu1 gene encodes the P. blakesleeanus alpha-IPM (isopropylmalate) isomerase. More... »

PAGES

215-223

References to SciGraph publications

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  • Journal

    TITLE

    Current Genetics

    ISSUE

    3

    VOLUME

    21

    Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00336844

    DOI

    http://dx.doi.org/10.1007/bf00336844

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015031379

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1563047


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