Ontology type: schema:ScholarlyArticle
1989-04
AUTHORSImerio Capone, Maura Cardarelli, Maurizio Trovato, Paolo Costantino
ABSTRACTRoot differentiation could be elicited on carrot discs by transformation with the agropine Ri plasmid rolB gene cloned in the binary vector Bin19, provided two conditions were met. Firstly, an adequate auxin supply had to be provided. This was achieved by co-inoculation with a strain carrying only the auxin synthetic genes of the TR-DNA. Most of the resulting roots were then shown to harbour only rolB and no aux genes. Secondly, an extended non-coding region (∼1200 bp) at the 5′ end of rolB had to be included in the construction. A shorter (∼300 bp) 5′ region, including TATA and CCAAT boxes, was not sufficient to trigger root differentiation. Both the extended (B1185) and reduced (B310) 5′ regions of rolB were then cloned upstream of the β-glucuronidase (GUS) reporter gene and infections carried out both on the apical and on the basal side of carrot discs. Strong expression of GUS, visualized histochemically as an intense blue colouring of transformed cells was observed with B1185-GUS constructions on the apical side of the discs. Only occasionally could coloured cells be observed on the basal side of the discs with B1185-GUS and on both apical and basal sides with B310-GUS constructions. Strong GUS expression was, on the contrary, achieved on cells of both auxin-rich (apical) and auxin-depleted (basal) sides of the discs with the strong constitutive viral promoter, CaMV35S. These results indicate the presence of an upstream regulatory region which confers polar expression to the rolB gene and suggest a role for auxin in its activation. More... »
PAGES239-244
http://scigraph.springernature.com/pub.10.1007/bf00334362
DOIhttp://dx.doi.org/10.1007/bf00334362
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1003471607
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Sapienza University of Rome",
"id": "https://www.grid.ac/institutes/grid.7841.a",
"name": [
"Dip. Genetica e Biologia Molecolare, Universit\u00e0 di Roma, \u201cLa Sapienza\u201d, P. le A. Moro 5, I-00185, Rome, Italy"
],
"type": "Organization"
},
"familyName": "Capone",
"givenName": "Imerio",
"id": "sg:person.01330005620.36",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330005620.36"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Research Council",
"id": "https://www.grid.ac/institutes/grid.5326.2",
"name": [
"Centro per lo Studio degli Acidi Nucleici, CNR, Rome, Italy"
],
"type": "Organization"
},
"familyName": "Cardarelli",
"givenName": "Maura",
"id": "sg:person.0624225332.08",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624225332.08"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sapienza University of Rome",
"id": "https://www.grid.ac/institutes/grid.7841.a",
"name": [
"Dip. Genetica e Biologia Molecolare, Universit\u00e0 di Roma, \u201cLa Sapienza\u201d, P. le A. Moro 5, I-00185, Rome, Italy"
],
"type": "Organization"
},
"familyName": "Trovato",
"givenName": "Maurizio",
"id": "sg:person.0765021707.36",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765021707.36"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sapienza University of Rome",
"id": "https://www.grid.ac/institutes/grid.7841.a",
"name": [
"Dip. Genetica e Biologia Molecolare, Universit\u00e0 di Roma, \u201cLa Sapienza\u201d, P. le A. Moro 5, I-00185, Rome, Italy"
],
"type": "Organization"
},
"familyName": "Costantino",
"givenName": "Paolo",
"id": "sg:person.01363600477.25",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363600477.25"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/bf00422906",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001986980",
"https://doi.org/10.1007/bf00422906"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00422906",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001986980",
"https://doi.org/10.1007/bf00422906"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00331152",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003334341",
"https://doi.org/10.1007/bf00331152"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00331152",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003334341",
"https://doi.org/10.1007/bf00331152"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.79.10.3193",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007604259"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00337765",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009190985",
"https://doi.org/10.1007/bf00337765"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00337765",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009190985",
"https://doi.org/10.1007/bf00337765"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1104/pp.77.1.215",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010849940"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0147-619x(82)90023-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014060774"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00027560",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028197432",
"https://doi.org/10.1007/bf00027560"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00027560",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028197432",
"https://doi.org/10.1007/bf00027560"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/295432a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033213612",
"https://doi.org/10.1038/295432a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00330641",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039368673",
"https://doi.org/10.1007/bf00330641"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00330641",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039368673",
"https://doi.org/10.1007/bf00330641"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0147-619x(85)90050-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040682792"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00328139",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041292539",
"https://doi.org/10.1007/bf00328139"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00328139",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041292539",
"https://doi.org/10.1007/bf00328139"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00037559",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049818271",
"https://doi.org/10.1007/bf00037559"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00037559",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049818271",
"https://doi.org/10.1007/bf00037559"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/12.22.8711",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053487532"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/00221287-98-2-477",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060376102"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1987.tb02729.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1077183971"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079501279",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1987.tb02730.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1079687101"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1080097975",
"type": "CreativeWork"
}
],
"datePublished": "1989-04",
"datePublishedReg": "1989-04-01",
"description": "Root differentiation could be elicited on carrot discs by transformation with the agropine Ri plasmid rolB gene cloned in the binary vector Bin19, provided two conditions were met. Firstly, an adequate auxin supply had to be provided. This was achieved by co-inoculation with a strain carrying only the auxin synthetic genes of the TR-DNA. Most of the resulting roots were then shown to harbour only rolB and no aux genes. Secondly, an extended non-coding region (\u223c1200 bp) at the 5\u2032 end of rolB had to be included in the construction. A shorter (\u223c300 bp) 5\u2032 region, including TATA and CCAAT boxes, was not sufficient to trigger root differentiation. Both the extended (B1185) and reduced (B310) 5\u2032 regions of rolB were then cloned upstream of the \u03b2-glucuronidase (GUS) reporter gene and infections carried out both on the apical and on the basal side of carrot discs. Strong expression of GUS, visualized histochemically as an intense blue colouring of transformed cells was observed with B1185-GUS constructions on the apical side of the discs. Only occasionally could coloured cells be observed on the basal side of the discs with B1185-GUS and on both apical and basal sides with B310-GUS constructions. Strong GUS expression was, on the contrary, achieved on cells of both auxin-rich (apical) and auxin-depleted (basal) sides of the discs with the strong constitutive viral promoter, CaMV35S. These results indicate the presence of an upstream regulatory region which confers polar expression to the rolB gene and suggest a role for auxin in its activation.",
"genre": "research_article",
"id": "sg:pub.10.1007/bf00334362",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1297380",
"issn": [
"1617-4615",
"1432-1874"
],
"name": "Molecular Genetics and Genomics",
"type": "Periodical"
},
{
"issueNumber": "2-3",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "216"
}
],
"name": "Upstream non-coding region which confers polar expression to Ri plasmid root inducing gene rolB",
"pagination": "239-244",
"productId": [
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00334362"
]
},
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"1089d68def3d55937c13338ea3f0664268a3d9df9fc604db33589d68fc6aa8f0"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1003471607"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00334362",
"https://app.dimensions.ai/details/publication/pub.1003471607"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-15T08:48",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119720_00000000.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/BF00334362"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00334362'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00334362'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00334362'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00334362'
This table displays all metadata directly associated to this object as RDF triples.
145 TRIPLES
21 PREDICATES
45 URIs
19 LITERALS
7 BLANK NODES