Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-07

AUTHORS

Brigitte Damm, Lothar Willmitzer

ABSTRACT

A protocol for obtaining regenerated fertile plants from mesophyll protoplasts of Arabidopsis thaliana is reported. Protoplasts were isolated from leaves of 21-to 28-day-old Arabidopsis plants grown in a controlled environment. Sustained divisions were achieved when protoplasts were embedded in beads formed by 1.4% sodium alginate in the presence of 50mM CaCl2 in 0.4 mannitol, which was then exchanged againts modified B5 medium. About 0.4%–0.6% of the protoplasts developed into colonies of which 80%–90% formed shoots and subsequently regenerated to fertile plants. Seeds harvested from more than 200 independently regenerated plants were sown and germination frequencies of more than 95% were obtained. Furthermore, the F1 plants did not show any evidence of somaclonal variation on visual inspection. This protocol was originally developed for Arabidopsis thaliana “Columbia”; however it was shown to be applicable also for the genotypes “Wassilewskija”, “Landsberg erecta” and “Estland” though with differing efficiencies. More... »

PAGES

15-20

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00333392

DOI

http://dx.doi.org/10.1007/bf00333392

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014686522


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