Thermostability and superhelicity of plasmid DNA in Bacillus stearothermophilus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-10

AUTHORS

Erwin Soutschek-Bauer, Winfried Scholz, Erwin Grill, Walter L. Staudenbauer

ABSTRACT

The thermostability of the staphylococcal plasmids pC194 and pUB110 and their antibiotic-resistance determinants was examined upon transfer to Bacillus stearothermophilus CU21. Plasmid pGS13, a pUB110 derivative carrying the chloramphenicol acetyltransferase (CAT) gene of pC194, could be maintained up to the maximum growth temperature (68° C) by selection for chloramphenicol resistance. In the absence of selective pressure, pGS13 was lost at temperatures above 60° C. Segregational instability of pGS13 was accompanied by a progressive loss of negative superhelicity at elevated temperatures. Thermostable mutants of pGS13 were isolated by screening for expression of the antibiotic-resistance determinants after growth under non-selective conditions. These mutants were found to contain an insertion of a 1.7 kb DNA sequence derived from the cryptic B. stearothermophilus plasmid pBS02. Increased thermostability correlated with preservation of plasmid superhelicity at elevated temperatures. More... »

PAGES

575-579

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00331165

DOI

http://dx.doi.org/10.1007/bf00331165

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035830575

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2828885


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