Gene expression in Streptomyces: Construction and application of promoter-probe plasmid vectors in Streptomyces lividans View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1982-10

AUTHORS

Mervyn J. Bibb, Stanley N. Cohen

ABSTRACT

Promoter-probe plasmid vectors were constructed for Streptomyces lividans using expression of the Escherichia coli chloramphenicol acetyltransferase gene as an indicator of promoter activity. These vectors have been used to isolate and to study the activity of DNA sequences that contain transcriptional control signals from Streptomyces, Bacillus licheniformis, E. coli, and Serratia marcescens. Studies of these promoter regions in heterospecific hosts indicate that genus or species-specific factors may present barriers to the expression of bacterial genetic material in certain heterologous cellular environments. While promoter regions isolated from E. coli, S. marcescens and B. licheniformis all appear to be recognized by the RNA polymerase of S. lividans, the Streptomyces transcriptional control signals isolated do not appear to function normally in E. coli. More... »

PAGES

265-277

References to SciGraph publications

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  • 1982. Gene cloning in Streptomyces. in GENE CLONING IN ORGANISMS OTHER THAN E. COLI
  • 1980-07. DNA cloning in Streptomyces: resistance genes from antibiotic-producing species in NATURE
  • 1979-12. Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9 in NATURE
  • 1982-04. pIJ101, a multi-copy broad host-range Streptomyces plasmid: Functional analysis and development of DNA cloning vectors in MOLECULAR GENETICS AND GENOMICS
  • 1979. Genetic Signals and Nucleotide Sequences in Messenger RNA in BIOLOGICAL REGULATION AND DEVELOPMENT
  • 1978-07. Transformation of plasmid DNA into Streptomyces at high frequency in NATURE
  • 1980-07. DNA cloning in Streptomyces: a bifunctional replicon comprising pBR322 inserted into a Streptomyces phage in NATURE
  • 1978-12. The regulatory region of the trp operon of Serratia marcescens in NATURE
  • 1977-01. Physical and genetical characterisation of a second sex factor, SCP2, for Streptomyces coelicolor A3(2) in MOLECULAR GENETICS AND GENOMICS
  • 1973-11. Improved Estimation of Secondary Structure in Ribonucleic Acids in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00331128

    DOI

    http://dx.doi.org/10.1007/bf00331128

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035940821

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/6294463


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