The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-09

AUTHORS

Csaba Koncz, Jeff Schell

ABSTRACT

A “plant gene vector cassette” to be used in combination with various Escherichia coli gene-cloning vectors was constructed. This cassette contains a replication and mobilization unit which allows it to be maintained and to be transferred back and forth between E. coli and Agrobacterium tumefaciens hosts provided these hosts contain plasmid RK2 replication and mobilization helper functions. The cassette also harbors a transferable DNA unit with plant selectable marker genes and cloning sites which can be combined with different bacterial replicons, thus facilitating the reisolation of transferred DNA from transformed plants in E. coli. The vector cassette contains two different promoters derived from the T-DNA-encoded genes 5 and nopaline synthase (NOS). By comparing the levels of expression of the marker enzymes linked to each of these promoter sequences, it was found that the gene 5 promoter is active in a tissue-specific fashion whereas this is not the case for the NOS promoter. This observation provides the first documented instance of a gene derived from a procaryotic host the expression of which is apparently regulated by plant growth factors. More... »

PAGES

383-396

References to SciGraph publications

  • 1985-08. Light-regulated and organ-specific expression of a wheat Cab gene in transgenic tobacco in NATURE
  • 1983-05-01. Mini–Ti: A New Vector Strategy for Plant Genetic Engineering in NATURE BIOTECHNOLOGY
  • 1983-11. Nucleotide sequence of the T-DNA region from theA grobacterium tumefaciens octopine Ti plasmid pTi15955 in PLANT MOLECULAR BIOLOGY
  • 1974-11. Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability in NATURE
  • 1983-05. Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector in NATURE
  • 1985-03-01. Light-Inducible Expression of a Chimeric Gene in Soybean Tissue Transformed with Agrobacterium in NATURE BIOTECHNOLOGY
  • 1985-05. Simple binary vectors for DNA transfer to plant cells in PLANT MOLECULAR BIOLOGY
  • 1985-12. Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens in NATURE
  • 1984. Genes Involved in Microbe-Plant Interactions in NONE
  • 1983-05. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid in NATURE
  • 1979-01. Isolation of a recombination deficient Agrobacterium tumefaciens mutant in MOLECULAR GENETICS AND GENOMICS
  • 1985-01. Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells in NATURE
  • 1983-11. A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria in NATURE BIOTECHNOLOGY
  • 1982-04. Plasmid ColE1 conjugal mobility: The nature of bom, a region required in cis for transfer in MOLECULAR GENETICS AND GENOMICS
  • 1985-05. Organ-specific expression of three leaf/stem specific cDNAs from potato is regulated by light and correlated with chloroplast development in MOLECULAR GENETICS AND GENOMICS
  • 1985-02. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter in NATURE
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • 1979-01. In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens in NATURE
  • 1985-07-01. Vectors for Transformation of Higher Plants in NATURE BIOTECHNOLOGY
  • 1985-02. T-DNA-encoded auxin formation in crown-gall cells in PLANTA
  • Journal

    TITLE

    Molecular Genetics and Genomics

    ISSUE

    3

    VOLUME

    204

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00331014

    DOI

    http://dx.doi.org/10.1007/bf00331014

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1013909718


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    72 mobilization helper functions
    73 mobilization unit
    74 nopaline synthase
    75 novel type
    76 observations
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    78 plant growth factors
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    81 plasmid RK2 replication
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    87 replicon
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    93 tissue-specific fashion
    94 transferable DNA unit
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