The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-09

AUTHORS

Csaba Koncz, Jeff Schell

ABSTRACT

A “plant gene vector cassette” to be used in combination with various Escherichia coli gene-cloning vectors was constructed. This cassette contains a replication and mobilization unit which allows it to be maintained and to be transferred back and forth between E. coli and Agrobacterium tumefaciens hosts provided these hosts contain plasmid RK2 replication and mobilization helper functions. The cassette also harbors a transferable DNA unit with plant selectable marker genes and cloning sites which can be combined with different bacterial replicons, thus facilitating the reisolation of transferred DNA from transformed plants in E. coli. The vector cassette contains two different promoters derived from the T-DNA-encoded genes 5 and nopaline synthase (NOS). By comparing the levels of expression of the marker enzymes linked to each of these promoter sequences, it was found that the gene 5 promoter is active in a tissue-specific fashion whereas this is not the case for the NOS promoter. This observation provides the first documented instance of a gene derived from a procaryotic host the expression of which is apparently regulated by plant growth factors. More... »

PAGES

383-396

References to SciGraph publications

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  • Journal

    TITLE

    Molecular Genetics and Genomics

    ISSUE

    3

    VOLUME

    204

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00331014

    DOI

    http://dx.doi.org/10.1007/bf00331014

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1013909718


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