Evidence for clustered pathogenicity genes in Xanthomonas campestris pv. campestris View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-05

AUTHORS

Peter Turner, Christine Barber, Michael Daniels

ABSTRACT

Plasmid pIJ3000 contains DNA from the plant pathogen Xanthomonas campestris pv. campestris which restores symptom production to a non-pathogenic mutant. pIJ3000 was mutagenised with the kanamycin resistance transposon Tn5 in Escherichia coli, and the sites of insertion were mapped physically. Derivatives of pIJ3000 containing Tn5 inserted in X. c. campestris DNA were introduced into a wild-type X. c. campestris strain, and the Tn5 insertions recombined into the genome by a marker exchange process. The resulting strains were tested for pathogenicity to turnip seedlings. The parts of the cloned DNA required for symptom production by the wild-type were defined by correlating the phenotype of each Tn5 insertion in the genome with its position in pIJ3000. This led to the identification of a region of approximately 10 kb consisting of a cluster of genes involved in pathogenicity. More... »

PAGES

338-343

Journal

TITLE

Molecular Genetics and Genomics

ISSUE

2

VOLUME

199

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00330277

DOI

http://dx.doi.org/10.1007/bf00330277

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040961342


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