Gene transfer to cereal cells mediated by protoplast transformation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-05

AUTHORS

Horst Lörz, Barbara Baker, Jeff Schell

ABSTRACT

Direct gene transfer to cereal cells was achieved by transformation of protoplasts with naked DNA. Protoplasts isolated from cultured cells of Triticum monococcum were incubated in the presence of polyethylene glycol (PEG) with circular and linear plasmid DNA. The pBR322-derived plasmid, pBL1103-4, contained a selectable chimeric gene comprising the protein coding region of the Tn5 aminogly-coside phosphotransferase type II gene (NPT II), the nopaline synthase promoter (pNOS) and the polyadenylation signal of the octopine synthase gene. Transformed cells were selected in medium containing kanamycin and identified by detection of aminoglycoside phosphotransferase II activity. More... »

PAGES

178-182

Journal

TITLE

Molecular Genetics and Genomics

ISSUE

2

VOLUME

199

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00330256

    DOI

    http://dx.doi.org/10.1007/bf00330256

    DIMENSIONS

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