Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-05

AUTHORS

Ingo Potrykus, Jerzy Paszkowski, Michael W. Saul, Jirina Petruska, Raymond D. Shillito

ABSTRACT

Two clones of N. tabacum, transformed to kanamycin resistance by direct transfer to protoplasts of a hybrid gene, consisting of the protein coding region from the bacterial gene for aminoglycoside phosphotransferase under the control of 5′/3′ expression signals from cauliflower mosaic virus gene VI, in the bacterial plasmid pUC8, have been subjected to a detailed genetic crossing analysis accompanied by Southern blot analysis and enzyme activity assays of representative offspring. The genetic data obtained from large populations of R1/F1 and R2/F2 offspring as well as from more than 20 subclones of each of the original transformants confirm that (a) one functional copy of the hybrid gene was stably integrated into chromosomal DNA of the original transformants, (b) that the gene normally was stably maintained during clonal proliferation, (c) that normally it is transmitted in a regular fashion (with exceptions) to sexual offspring, and (d) that it is inherited as a single dominant trait. Data from DNA hybridisation and enzyme assays confirm this interpretation. The functional gene is integrated together with several non-functional copies and bacterial plasmid sequences, which are inherited as one block together with the functional gene. More... »

PAGES

169-177

References to SciGraph publications

Journal

TITLE

Molecular Genetics and Genomics

ISSUE

2

VOLUME

199

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00330255

    DOI

    http://dx.doi.org/10.1007/bf00330255

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1036019563

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3860712


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