Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-05

AUTHORS

R. Hain, P. Stabel, A. P. Czernilofsky, H. H. Steinbiß, L. Herrera-Estrella, J. Schell

ABSTRACT

Genetic transformation of Nicotiana tabacum protoplasts was achieved by incubation of protoplasts with a plasmid DNA-calcium phosphate coprecipitate, followed by fusion of the protoplasts in the presence of polyvinyl alcohol and subsequent exposure to high pH. A derivative of the plasmid pBR322 containing a chimaeric gene, consisting of the nopaline synthase promoter, the coding region of the aminoglycoside phosphotransferase gene of Tn5 and the polyadenylation signal region of the octopine synthase gene, was used for these transformation experiments. This chimaeric gene confers resistance of transformed plant cells to kanamycin. This novel transformation procedure reproducibly yielded transformants at frequencies of approximately 0.01%. Aminoglycoside phosphotransferase II activity was detected in both transformed calli and in regenerated plants. DNA from some of the transformed clones was analyzed by Southern blot hybridization. The input DNA appears to be integrated into high molecular weight cellular DNA. Genetic analysis of one of the kanamycin resistant plants shows that the chimaeric gene is transmitted to the progeny as a single dominant trait in a Mendelian fashion. As a comparison the input DNA was also introduced into tobacco protoplasts using Agrobacterium tumefaciens and Ti-plasmid derived gene vectors. More... »

PAGES

161-168

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00330254

DOI

http://dx.doi.org/10.1007/bf00330254

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011873691


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, 5000, K\u00f6ln 30, FRG"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hain", 
        "givenName": "R.", 
        "id": "sg:person.015306350227.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015306350227.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, 5000, K\u00f6ln 30, FRG"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Stabel", 
        "givenName": "P.", 
        "id": "sg:person.0611517606.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611517606.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, 5000, K\u00f6ln 30, FRG"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Czernilofsky", 
        "givenName": "A. P.", 
        "id": "sg:person.063412466.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.063412466.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, 5000, K\u00f6ln 30, FRG"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Steinbi\u00df", 
        "givenName": "H. H.", 
        "id": "sg:person.07417170227.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07417170227.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ghent University", 
          "id": "https://www.grid.ac/institutes/grid.5342.0", 
          "name": [
            "Laboratorium voor Genetika, Rijksuniversiteit Gent, B-9000, Gent, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Herrera-Estrella", 
        "givenName": "L.", 
        "id": "sg:person.01154341423.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154341423.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ghent University", 
          "id": "https://www.grid.ac/institutes/grid.5342.0", 
          "name": [
            "Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, 5000, K\u00f6ln 30, FRG", 
            "Laboratorium voor Genetika, Rijksuniversiteit Gent, B-9000, Gent, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schell", 
        "givenName": "J.", 
        "id": "sg:person.016705276677.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016705276677.96"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0378-1119(84)90122-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000852526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(84)90122-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000852526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/251687a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001747773", 
          "https://doi.org/10.1038/251687a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/251687a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001747773", 
          "https://doi.org/10.1038/251687a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00332778", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006970381", 
          "https://doi.org/10.1007/bf00332778"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00332778", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006970381", 
          "https://doi.org/10.1007/bf00332778"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(77)90052-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010609967"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1965.tb06874.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016020981"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.78.7.4344", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016290600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269659", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016477082", 
          "https://doi.org/10.1007/bf00269659"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269659", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016477082", 
          "https://doi.org/10.1007/bf00269659"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(78)90064-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018124965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4211(80)90121-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019406930"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244029a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022855491", 
          "https://doi.org/10.1038/newbio244029a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244029a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022855491", 
          "https://doi.org/10.1038/newbio244029a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244029a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022855491", 
          "https://doi.org/10.1038/newbio244029a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(80)90095-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024871846"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0044-328x(76)80093-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024944233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(81)90143-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026467580"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00270972", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028497677", 
          "https://doi.org/10.1007/bf00270972"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00270972", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028497677", 
          "https://doi.org/10.1007/bf00270972"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/304184a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030766221", 
          "https://doi.org/10.1038/304184a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/277129a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032852748", 
          "https://doi.org/10.1038/277129a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033296365"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00368577", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035165278", 
          "https://doi.org/10.1007/bf00368577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00368577", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035165278", 
          "https://doi.org/10.1007/bf00368577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.80.15.4803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035204516"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4211(83)90216-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037562307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4211(83)90216-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037562307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269151", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040500001", 
          "https://doi.org/10.1007/bf00269151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269151", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040500001", 
          "https://doi.org/10.1007/bf00269151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0044-328x(83)80056-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043859486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/303209a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051577901", 
          "https://doi.org/10.1038/303209a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/296072a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052220023", 
          "https://doi.org/10.1038/296072a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1974.tb03599.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052877810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/znc-1973-11-1215", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067583450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1983.tb01532.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1983.tb01715.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1984.tb02032.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1984.tb02201.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183716"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1984.tb02202.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269230", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078816517", 
          "https://doi.org/10.1007/bf00269230"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1985-05", 
    "datePublishedReg": "1985-05-01", 
    "description": "Genetic transformation of Nicotiana tabacum protoplasts was achieved by incubation of protoplasts with a plasmid DNA-calcium phosphate coprecipitate, followed by fusion of the protoplasts in the presence of polyvinyl alcohol and subsequent exposure to high pH. A derivative of the plasmid pBR322 containing a chimaeric gene, consisting of the nopaline synthase promoter, the coding region of the aminoglycoside phosphotransferase gene of Tn5 and the polyadenylation signal region of the octopine synthase gene, was used for these transformation experiments. This chimaeric gene confers resistance of transformed plant cells to kanamycin. This novel transformation procedure reproducibly yielded transformants at frequencies of approximately 0.01%. Aminoglycoside phosphotransferase II activity was detected in both transformed calli and in regenerated plants. DNA from some of the transformed clones was analyzed by Southern blot hybridization. The input DNA appears to be integrated into high molecular weight cellular DNA. Genetic analysis of one of the kanamycin resistant plants shows that the chimaeric gene is transmitted to the progeny as a single dominant trait in a Mendelian fashion. As a comparison the input DNA was also introduced into tobacco protoplasts using Agrobacterium tumefaciens and Ti-plasmid derived gene vectors.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00330254", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297380", 
        "issn": [
          "1617-4615", 
          "1432-1874"
        ], 
        "name": "Molecular Genetics and Genomics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "199"
      }
    ], 
    "name": "Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts", 
    "pagination": "161-168", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00330254"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3892bb5698aaa79d24611af70a9c466e875403652731a6095175ddfc533d0971"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1011873691"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00330254", 
      "https://app.dimensions.ai/details/publication/pub.1011873691"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T08:51", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119737_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00330254"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00330254'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00330254'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00330254'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00330254'


 

This table displays all metadata directly associated to this object as RDF triples.

216 TRIPLES      21 PREDICATES      60 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00330254 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N0860e7da84f549b7849d64c52331ee73
4 schema:citation sg:pub.10.1007/bf00269151
5 sg:pub.10.1007/bf00269230
6 sg:pub.10.1007/bf00269659
7 sg:pub.10.1007/bf00270972
8 sg:pub.10.1007/bf00332778
9 sg:pub.10.1007/bf00368577
10 sg:pub.10.1038/251687a0
11 sg:pub.10.1038/277129a0
12 sg:pub.10.1038/296072a0
13 sg:pub.10.1038/303209a0
14 sg:pub.10.1038/304184a0
15 sg:pub.10.1038/newbio244029a0
16 https://doi.org/10.1002/j.1460-2075.1983.tb01532.x
17 https://doi.org/10.1002/j.1460-2075.1983.tb01715.x
18 https://doi.org/10.1002/j.1460-2075.1984.tb02032.x
19 https://doi.org/10.1002/j.1460-2075.1984.tb02201.x
20 https://doi.org/10.1002/j.1460-2075.1984.tb02202.x
21 https://doi.org/10.1016/0003-2697(76)90527-3
22 https://doi.org/10.1016/0022-2836(77)90052-3
23 https://doi.org/10.1016/0022-2836(80)90095-9
24 https://doi.org/10.1016/0092-8674(78)90064-8
25 https://doi.org/10.1016/0304-4211(80)90121-2
26 https://doi.org/10.1016/0304-4211(83)90216-x
27 https://doi.org/10.1016/0378-1119(81)90143-8
28 https://doi.org/10.1016/0378-1119(84)90122-7
29 https://doi.org/10.1016/s0022-2836(75)80083-0
30 https://doi.org/10.1016/s0044-328x(76)80093-1
31 https://doi.org/10.1016/s0044-328x(83)80056-7
32 https://doi.org/10.1073/pnas.78.7.4344
33 https://doi.org/10.1073/pnas.80.15.4803
34 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x
35 https://doi.org/10.1111/j.1432-1033.1974.tb03599.x
36 https://doi.org/10.1515/znc-1973-11-1215
37 schema:datePublished 1985-05
38 schema:datePublishedReg 1985-05-01
39 schema:description Genetic transformation of Nicotiana tabacum protoplasts was achieved by incubation of protoplasts with a plasmid DNA-calcium phosphate coprecipitate, followed by fusion of the protoplasts in the presence of polyvinyl alcohol and subsequent exposure to high pH. A derivative of the plasmid pBR322 containing a chimaeric gene, consisting of the nopaline synthase promoter, the coding region of the aminoglycoside phosphotransferase gene of Tn5 and the polyadenylation signal region of the octopine synthase gene, was used for these transformation experiments. This chimaeric gene confers resistance of transformed plant cells to kanamycin. This novel transformation procedure reproducibly yielded transformants at frequencies of approximately 0.01%. Aminoglycoside phosphotransferase II activity was detected in both transformed calli and in regenerated plants. DNA from some of the transformed clones was analyzed by Southern blot hybridization. The input DNA appears to be integrated into high molecular weight cellular DNA. Genetic analysis of one of the kanamycin resistant plants shows that the chimaeric gene is transmitted to the progeny as a single dominant trait in a Mendelian fashion. As a comparison the input DNA was also introduced into tobacco protoplasts using Agrobacterium tumefaciens and Ti-plasmid derived gene vectors.
40 schema:genre research_article
41 schema:inLanguage en
42 schema:isAccessibleForFree false
43 schema:isPartOf Nbd84399f73dc4058855c9efbd40ef572
44 Nc3d5c8f9154a44de87c30fa3e7b0d955
45 sg:journal.1297380
46 schema:name Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts
47 schema:pagination 161-168
48 schema:productId N1b91f25b44ba49dfbe9f305bf18dfb3c
49 N25de650296f94f9fa8a636966120ce9c
50 N86f3f884a3e74609b9c5e71dd108422c
51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011873691
52 https://doi.org/10.1007/bf00330254
53 schema:sdDatePublished 2019-04-15T08:51
54 schema:sdLicense https://scigraph.springernature.com/explorer/license/
55 schema:sdPublisher Na4834af28c7e4c6faaa11d8e24541547
56 schema:url http://link.springer.com/10.1007/BF00330254
57 sgo:license sg:explorer/license/
58 sgo:sdDataset articles
59 rdf:type schema:ScholarlyArticle
60 N0860e7da84f549b7849d64c52331ee73 rdf:first sg:person.015306350227.66
61 rdf:rest N67fe868d72a04ebaa5d78b2b4ad7aca3
62 N0a8c8ab496c44d36a0d660eef3d0bba5 rdf:first sg:person.01154341423.52
63 rdf:rest N25722eb5b4d04d4b9b40ffa12cfea235
64 N1b91f25b44ba49dfbe9f305bf18dfb3c schema:name readcube_id
65 schema:value 3892bb5698aaa79d24611af70a9c466e875403652731a6095175ddfc533d0971
66 rdf:type schema:PropertyValue
67 N25722eb5b4d04d4b9b40ffa12cfea235 rdf:first sg:person.016705276677.96
68 rdf:rest rdf:nil
69 N25de650296f94f9fa8a636966120ce9c schema:name doi
70 schema:value 10.1007/bf00330254
71 rdf:type schema:PropertyValue
72 N3ecf87bfdd2f4d93b48ac0dbf5c7ae4c schema:name Max-Planck-Institut für Züchtungsforschung, 5000, Köln 30, FRG
73 rdf:type schema:Organization
74 N4f456dc24dd141caa2d0586d7e92f64c schema:name Max-Planck-Institut für Züchtungsforschung, 5000, Köln 30, FRG
75 rdf:type schema:Organization
76 N67fe868d72a04ebaa5d78b2b4ad7aca3 rdf:first sg:person.0611517606.58
77 rdf:rest Nb73e18cce8154a5d9b4b86901dfe7a06
78 N86f3f884a3e74609b9c5e71dd108422c schema:name dimensions_id
79 schema:value pub.1011873691
80 rdf:type schema:PropertyValue
81 N90fff87111d04f49b32e0ce058a0d897 schema:name Max-Planck-Institut für Züchtungsforschung, 5000, Köln 30, FRG
82 rdf:type schema:Organization
83 Na4834af28c7e4c6faaa11d8e24541547 schema:name Springer Nature - SN SciGraph project
84 rdf:type schema:Organization
85 Nb73e18cce8154a5d9b4b86901dfe7a06 rdf:first sg:person.063412466.34
86 rdf:rest Nf88ce88fc3bd42799cd34b6bb3489efc
87 Nbd84399f73dc4058855c9efbd40ef572 schema:issueNumber 2
88 rdf:type schema:PublicationIssue
89 Nbdf3b38432a84c89bb20d028f8f4f083 schema:name Max-Planck-Institut für Züchtungsforschung, 5000, Köln 30, FRG
90 rdf:type schema:Organization
91 Nc3d5c8f9154a44de87c30fa3e7b0d955 schema:volumeNumber 199
92 rdf:type schema:PublicationVolume
93 Nf88ce88fc3bd42799cd34b6bb3489efc rdf:first sg:person.07417170227.49
94 rdf:rest N0a8c8ab496c44d36a0d660eef3d0bba5
95 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
96 schema:name Biological Sciences
97 rdf:type schema:DefinedTerm
98 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
99 schema:name Genetics
100 rdf:type schema:DefinedTerm
101 sg:journal.1297380 schema:issn 1432-1874
102 1617-4615
103 schema:name Molecular Genetics and Genomics
104 rdf:type schema:Periodical
105 sg:person.01154341423.52 schema:affiliation https://www.grid.ac/institutes/grid.5342.0
106 schema:familyName Herrera-Estrella
107 schema:givenName L.
108 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154341423.52
109 rdf:type schema:Person
110 sg:person.015306350227.66 schema:affiliation N3ecf87bfdd2f4d93b48ac0dbf5c7ae4c
111 schema:familyName Hain
112 schema:givenName R.
113 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015306350227.66
114 rdf:type schema:Person
115 sg:person.016705276677.96 schema:affiliation https://www.grid.ac/institutes/grid.5342.0
116 schema:familyName Schell
117 schema:givenName J.
118 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016705276677.96
119 rdf:type schema:Person
120 sg:person.0611517606.58 schema:affiliation Nbdf3b38432a84c89bb20d028f8f4f083
121 schema:familyName Stabel
122 schema:givenName P.
123 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611517606.58
124 rdf:type schema:Person
125 sg:person.063412466.34 schema:affiliation N4f456dc24dd141caa2d0586d7e92f64c
126 schema:familyName Czernilofsky
127 schema:givenName A. P.
128 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.063412466.34
129 rdf:type schema:Person
130 sg:person.07417170227.49 schema:affiliation N90fff87111d04f49b32e0ce058a0d897
131 schema:familyName Steinbiß
132 schema:givenName H. H.
133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07417170227.49
134 rdf:type schema:Person
135 sg:pub.10.1007/bf00269151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040500001
136 https://doi.org/10.1007/bf00269151
137 rdf:type schema:CreativeWork
138 sg:pub.10.1007/bf00269230 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078816517
139 https://doi.org/10.1007/bf00269230
140 rdf:type schema:CreativeWork
141 sg:pub.10.1007/bf00269659 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016477082
142 https://doi.org/10.1007/bf00269659
143 rdf:type schema:CreativeWork
144 sg:pub.10.1007/bf00270972 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028497677
145 https://doi.org/10.1007/bf00270972
146 rdf:type schema:CreativeWork
147 sg:pub.10.1007/bf00332778 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006970381
148 https://doi.org/10.1007/bf00332778
149 rdf:type schema:CreativeWork
150 sg:pub.10.1007/bf00368577 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035165278
151 https://doi.org/10.1007/bf00368577
152 rdf:type schema:CreativeWork
153 sg:pub.10.1038/251687a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001747773
154 https://doi.org/10.1038/251687a0
155 rdf:type schema:CreativeWork
156 sg:pub.10.1038/277129a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032852748
157 https://doi.org/10.1038/277129a0
158 rdf:type schema:CreativeWork
159 sg:pub.10.1038/296072a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052220023
160 https://doi.org/10.1038/296072a0
161 rdf:type schema:CreativeWork
162 sg:pub.10.1038/303209a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051577901
163 https://doi.org/10.1038/303209a0
164 rdf:type schema:CreativeWork
165 sg:pub.10.1038/304184a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030766221
166 https://doi.org/10.1038/304184a0
167 rdf:type schema:CreativeWork
168 sg:pub.10.1038/newbio244029a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022855491
169 https://doi.org/10.1038/newbio244029a0
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1002/j.1460-2075.1983.tb01532.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183600
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1002/j.1460-2075.1983.tb01715.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183620
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1002/j.1460-2075.1984.tb02032.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183680
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1002/j.1460-2075.1984.tb02201.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183716
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1002/j.1460-2075.1984.tb02202.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183717
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/0022-2836(77)90052-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010609967
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/0022-2836(80)90095-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024871846
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/0092-8674(78)90064-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018124965
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/0304-4211(80)90121-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019406930
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/0304-4211(83)90216-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037562307
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/0378-1119(81)90143-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026467580
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/0378-1119(84)90122-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000852526
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/s0044-328x(76)80093-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024944233
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/s0044-328x(83)80056-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043859486
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1073/pnas.78.7.4344 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016290600
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1073/pnas.80.15.4803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035204516
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016020981
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1111/j.1432-1033.1974.tb03599.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1052877810
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1515/znc-1973-11-1215 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067583450
212 rdf:type schema:CreativeWork
213 https://www.grid.ac/institutes/grid.5342.0 schema:alternateName Ghent University
214 schema:name Laboratorium voor Genetika, Rijksuniversiteit Gent, B-9000, Gent, Belgium
215 Max-Planck-Institut für Züchtungsforschung, 5000, Köln 30, FRG
216 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...