Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-07

AUTHORS

P. Gamas, A. C. Burger, G. Churchward, L. Caro, D. Galas, M. Chandler

ABSTRACT

We have shown that the plasmid pSC101 is unable to be maintained in strains of E. coli carrying deletions in the genes himA and hip which specify the pleitropic heterodimeric DNA binding protein, IHF. We show that this effect is not due to a modulation of the expression of the pSC101 RepA protein, required for replication of the plasmid. Inspection of the DNA sequence of the essential replication region of pSC101 reveals the presence of a site, located between the DnaA binding-site and that of RepA, which shows extensive homology with the consensus IHF binding site. The proximity of the sites suggests that these three proteins, IHF, DnaA, and RepA may interact in generating a specific DNA structure required for initiation of pSC101 replication. More... »

PAGES

85-89

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00330192

DOI

http://dx.doi.org/10.1007/bf00330192

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050794972

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3528758


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