Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-07

AUTHORS

Yoshizumi Ishino, Hideo Shinagawa, Kozo Makino, Susumu Tsunasawa, Fumio Sakiyama, Atsuo Nakata

ABSTRACT

The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5′-phosphoryl and 3′-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690. More... »

PAGES

1-7

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00330179

DOI

http://dx.doi.org/10.1007/bf00330179

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034142000

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3018436


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