Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1975-03

AUTHORS

Carll Goodpasture, Stephen E. Bloom

ABSTRACT

A simple ammoniacal silver staining procedure, designated Ag-AS, differentially stains the chromosomal locations of ribosomal DNA in certain mammalian species. This was critically demonstrated by Ag-AS staining of the nucleolus organizer regions in karyotypes of the same species and cell lines used for locating the ribosomal cistrons by DNA/RNA in situ hybridization. With Ag-AS, silver stained NORs (Ag-NORs) are visualized as black spherical bodies on yellow-brown chromosome arms. Ag-NORs were visualized throughout mitosis at the secondary constrictions in the rat kangaroo, Seba's fruit bat, Indian muntjac, and Rhesus monkey. The Chinese hamster and cattle have telomeric Ag-NORs, the mouse subcentromeric Ag-NORs, and the field vole Ag-NORs as minute short arms or choromosomal satellites. Ag-NORs occur at both secondary constrictions and at telomeres in the cotton rat. Variability in Ag-NOR pattern included differences in the number of Ag-NORs per cell within a cell population, size of Ag-NORs among chromosomes of a complement, and presence of Ag-NOR on particular chromosomes in two cell lines of the Chinese hamster. The available cytochemical data suggest that the Ag-AS reaction stains chromosomal proteins at the NOR rather than the rDNA itself. More... »

PAGES

37-50

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00329389

DOI

http://dx.doi.org/10.1007/bf00329389

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049663508

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/53131


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