Inverted repeats in the DNA of Streptomyces plasmids pMG110 and pMG120 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1984-10

AUTHORS

Siegfried Klaus, Hans Krügel, Friedrich Walter

ABSTRACT

Two cryptic plasmids pMG110 (10.5 kb) and pMG120 (14.5 kb) isolated from Streptomyces luteolutescens were cleaved by restriction endonucleases BglII, KpnI, and SalGI. A physical map was constructed for pMG110. After denaturation and intrastrand reannealing, two types of snap-back structures were identified by electron microscopy, differing in the size of the loop (type 1, 1 kb; type 2, 1.6 kb), whereas the stem of both structures was about 190 bp long. Stem-loop structures of similar size were also observed in pMG120. In rare cases, both types of elements were present on the same DNA molecule. The analysis of BglII- and KpnI-generated fragments allowed the localization of the elements at two alternative positions on the physical map of pMG110. More... »

PAGES

143-149

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00327935

DOI

http://dx.doi.org/10.1007/bf00327935

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053598988

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6096671


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