Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-04

AUTHORS

Mervyn J. Bibb, Maureen J. Bibb, Judy M. Ward, Stanley N. Cohen

ABSTRACT

Promoter-probe plasmid vectors were used to isolate putative promoter-containing DNA fragments of three Streptomyces antibiotic resistance genes, the rRNA methylase (tsr) gene of S. azureus, the aminoglycoside phosphotransferase (aph) gene of S. fradiae, and the viomycin phosphotransferase (vph) gene of S. vinaceus. DNA sequence analysis was carried out for all three of the fragments and for the protein-coding regions of the tsr and vph genes. No sequences resembling typical E. coli promoters or Bacillus vegetatively-expressed promoters were identified. Furthermore, none of the three DNA fragments found to be transcriptionally active in Streptomyces could initiate transcription when introduced into E. coli. An extremely biased codon usage pattern that reflects the high G + C composition of Streptomyces DNA was observed for the protein-coding regions of the tsr and vph genes, and of the previously sequenced aph gene. This pattern enabled delineation of the protein-coding region and identification of the coding strand of the genes. More... »

PAGES

26-36

References to SciGraph publications

  • 1984-06. Coupled transcription — translation in extracts of Streptomyces lividans in MOLECULAR GENETICS AND GENOMICS
  • 1980-04. A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces in NATURE
  • 1977-07. Genetic recombination through protoplast fusion in Streptomyces in NATURE
  • 1973-11. Improved Estimation of Secondary Structure in Ribonucleic Acids in NATURE
  • 1978-01. Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6 in MOLECULAR GENETICS AND GENOMICS
  • 1982. Gene cloning in Streptomyces. in GENE CLONING IN ORGANISMS OTHER THAN E. COLI
  • 1973-03. Location of DNA methylation genes on the Escherichia coli K-12 genetic map in MOLECULAR GENETICS AND GENOMICS
  • 1980-07. DNA cloning in Streptomyces: resistance genes from antibiotic-producing species in NATURE
  • 1982-10. Gene expression in Streptomyces: Construction and application of promoter-probe plasmid vectors in Streptomyces lividans in MOLECULAR GENETICS AND GENOMICS
  • 1982-04. pIJ101, a multi-copy broad host-range Streptomyces plasmid: Functional analysis and development of DNA cloning vectors in MOLECULAR GENETICS AND GENOMICS
  • 1978-07. Transformation of plasmid DNA into Streptomyces at high frequency in NATURE
  • 1977-01. Lambdoid phages that simplify the recovery of in vitro recombinants in MOLECULAR GENETICS AND GENOMICS
  • 1985-01. RNA polymerase heterogeneity in Streptomyces coelicolor in NATURE
  • 1977-01. Physical and genetical characterisation of a second sex factor, SCP2, for Streptomyces coelicolor A3(2) in MOLECULAR GENETICS AND GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00327505

    DOI

    http://dx.doi.org/10.1007/bf00327505

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1039618263

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2987648


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Codon", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Drug Resistance, Microbial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Kanamycin Kinase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Methyltransferases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phosphotransferases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Promoter Regions, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Species Specificity", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Streptomyces", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transcription, Genetic", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "John Innes Institute, Colney Lane, NR4 7UH, Norwich, United Kingdom", 
                "Department of Genetics, Stanford University School of Medicine, 94305, Stanford, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bibb", 
            "givenName": "Mervyn J.", 
            "id": "sg:person.0612653332.06", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612653332.06"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "John Innes Centre", 
              "id": "https://www.grid.ac/institutes/grid.14830.3e", 
              "name": [
                "John Innes Institute, Colney Lane, NR4 7UH, Norwich, United Kingdom"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bibb", 
            "givenName": "Maureen J.", 
            "id": "sg:person.014221666144.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014221666144.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "John Innes Centre", 
              "id": "https://www.grid.ac/institutes/grid.14830.3e", 
              "name": [
                "John Innes Institute, Colney Lane, NR4 7UH, Norwich, United Kingdom"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ward", 
            "givenName": "Judy M.", 
            "id": "sg:person.01073762703.99", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073762703.99"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Department of Genetics, Stanford University School of Medicine, 94305, Stanford, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cohen", 
            "givenName": "Stanley N.", 
            "id": "sg:person.01263331517.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263331517.27"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1146/annurev.ge.13.120179.001535", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005203403"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(80)65059-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008420927"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.75.3.1433", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008922793"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.mi.35.100181.002053", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009116890"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(82)90086-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012114442"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(82)90086-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012114442"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330791", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015265907", 
              "https://doi.org/10.1007/bf00330791"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330791", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015265907", 
              "https://doi.org/10.1007/bf00330791"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/newbio246040a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018988773", 
              "https://doi.org/10.1038/newbio246040a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/newbio246040a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018988773", 
              "https://doi.org/10.1038/newbio246040a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-2836(74)90357-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019434826"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(83)90174-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021221912"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(83)90174-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021221912"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/286525a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022878906", 
              "https://doi.org/10.1038/286525a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00332721", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023826417", 
              "https://doi.org/10.1007/bf00332721"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00332721", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023826417", 
              "https://doi.org/10.1007/bf00332721"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.77.9.5201", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024100683"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330831", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029070617", 
              "https://doi.org/10.1007/bf00330831"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330831", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029070617", 
              "https://doi.org/10.1007/bf00330831"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02425325", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029286084", 
              "https://doi.org/10.1007/bf02425325"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02425325", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029286084", 
              "https://doi.org/10.1007/bf02425325"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/284526a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035072504", 
              "https://doi.org/10.1038/284526a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/11.8.2237", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035258827"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00331128", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035940821", 
              "https://doi.org/10.1007/bf00331128"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00331128", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035940821", 
              "https://doi.org/10.1007/bf00331128"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/274398a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037819258", 
              "https://doi.org/10.1038/274398a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.69.8.2110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038510205"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/313022a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041065614", 
              "https://doi.org/10.1038/313022a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.70.11.3240", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041686183"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/268171a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041989527", 
              "https://doi.org/10.1038/268171a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj1040258", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043664260"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj1040258", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043664260"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/b978-0-12-372380-2.50008-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045923569"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00267869", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045931431", 
              "https://doi.org/10.1007/bf00267869"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00267869", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045931431", 
              "https://doi.org/10.1007/bf00267869"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(84)90090-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046284362"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(84)90090-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046284362"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/7.6.1513", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046335196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00267782", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046612795", 
              "https://doi.org/10.1007/bf00267782"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00267782", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046612795", 
              "https://doi.org/10.1007/bf00267782"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0968-0004(78)95527-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047328323"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.71.4.1342", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048283731"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.80.17.5190", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049963307"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(84)90116-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051432348"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(84)90116-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051432348"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(80)65055-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051624275"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/00221287-129-9-2703", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060364998"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/00221287-80-2-389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060375149"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080157422", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080457401", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080645369", 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-68315-2_5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1081656921", 
              "https://doi.org/10.1007/978-3-642-68315-2_5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081661037", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081661038", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081963372", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082099856", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1985-04", 
        "datePublishedReg": "1985-04-01", 
        "description": "Promoter-probe plasmid vectors were used to isolate putative promoter-containing DNA fragments of three Streptomyces antibiotic resistance genes, the rRNA methylase (tsr) gene of S. azureus, the aminoglycoside phosphotransferase (aph) gene of S. fradiae, and the viomycin phosphotransferase (vph) gene of S. vinaceus. DNA sequence analysis was carried out for all three of the fragments and for the protein-coding regions of the tsr and vph genes. No sequences resembling typical E. coli promoters or Bacillus vegetatively-expressed promoters were identified. Furthermore, none of the three DNA fragments found to be transcriptionally active in Streptomyces could initiate transcription when introduced into E. coli. An extremely biased codon usage pattern that reflects the high G + C composition of Streptomyces DNA was observed for the protein-coding regions of the tsr and vph genes, and of the previously sequenced aph gene. This pattern enabled delineation of the protein-coding region and identification of the coding strand of the genes.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00327505", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2449214", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2633631", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1297380", 
            "issn": [
              "1617-4615", 
              "1432-1874"
            ], 
            "name": "Molecular Genetics and Genomics", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "199"
          }
        ], 
        "name": "Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces", 
        "pagination": "26-36", 
        "productId": [
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00327505"
            ]
          }, 
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "bcb8e8c92489952cae1acf62b909ed77957cefd8d35b941d0154b20975fc3b84"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1039618263"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0125036"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "2987648"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00327505", 
          "https://app.dimensions.ai/details/publication/pub.1039618263"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-15T08:50", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119730_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00327505"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00327505'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00327505'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00327505'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00327505'


     

    This table displays all metadata directly associated to this object as RDF triples.

    290 TRIPLES      21 PREDICATES      86 URIs      35 LITERALS      23 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00327505 schema:about N03773a66a4604ff68f113db6bff90563
    2 N179cb77702334c688db2ad4286311778
    3 N280ce2b7c2024ee78a24ba13125b8d68
    4 N35487ee4ff854e0993b6dc8c641082f9
    5 N37c4a7b6daee4fe08310243722d4cccf
    6 N388a7ef40dcc442c853687d3d77b8c03
    7 N40841c666d504da88222fbcccbc17fa8
    8 N6562d5160fb041289a077df1b607577b
    9 N9b87e84fa0974676a9f7fe43848d20a8
    10 N9c7a61152067482ebfa701d8a8e22fae
    11 Nb2d90029962e48e2a93f1fb07c4404d2
    12 Nb72927ef4f9b46f18ad95e6219783698
    13 Nda71ec1b59284bf6a1d3dec2cb757d90
    14 Ne0688890879e4db49e65d7944b465825
    15 anzsrc-for:06
    16 anzsrc-for:0604
    17 schema:author Ncd1bc8eb0539404d8a53fcda1c435fa2
    18 schema:citation sg:pub.10.1007/978-3-642-68315-2_5
    19 sg:pub.10.1007/bf00267782
    20 sg:pub.10.1007/bf00267869
    21 sg:pub.10.1007/bf00330791
    22 sg:pub.10.1007/bf00330831
    23 sg:pub.10.1007/bf00331128
    24 sg:pub.10.1007/bf00332721
    25 sg:pub.10.1007/bf02425325
    26 sg:pub.10.1038/268171a0
    27 sg:pub.10.1038/274398a0
    28 sg:pub.10.1038/284526a0
    29 sg:pub.10.1038/286525a0
    30 sg:pub.10.1038/313022a0
    31 sg:pub.10.1038/newbio246040a0
    32 https://app.dimensions.ai/details/publication/pub.1080157422
    33 https://app.dimensions.ai/details/publication/pub.1080457401
    34 https://app.dimensions.ai/details/publication/pub.1080645369
    35 https://app.dimensions.ai/details/publication/pub.1081661037
    36 https://app.dimensions.ai/details/publication/pub.1081661038
    37 https://app.dimensions.ai/details/publication/pub.1081963372
    38 https://app.dimensions.ai/details/publication/pub.1082099856
    39 https://doi.org/10.1016/0022-2836(74)90357-x
    40 https://doi.org/10.1016/0378-1119(82)90086-5
    41 https://doi.org/10.1016/0378-1119(83)90174-9
    42 https://doi.org/10.1016/0378-1119(84)90090-8
    43 https://doi.org/10.1016/0378-1119(84)90116-1
    44 https://doi.org/10.1016/b978-0-12-372380-2.50008-1
    45 https://doi.org/10.1016/s0076-6879(80)65055-1
    46 https://doi.org/10.1016/s0076-6879(80)65059-9
    47 https://doi.org/10.1016/s0968-0004(78)95527-5
    48 https://doi.org/10.1042/bj1040258
    49 https://doi.org/10.1073/pnas.69.8.2110
    50 https://doi.org/10.1073/pnas.70.11.3240
    51 https://doi.org/10.1073/pnas.71.4.1342
    52 https://doi.org/10.1073/pnas.75.3.1433
    53 https://doi.org/10.1073/pnas.77.9.5201
    54 https://doi.org/10.1073/pnas.80.17.5190
    55 https://doi.org/10.1093/nar/11.8.2237
    56 https://doi.org/10.1093/nar/7.6.1513
    57 https://doi.org/10.1099/00221287-129-9-2703
    58 https://doi.org/10.1099/00221287-80-2-389
    59 https://doi.org/10.1146/annurev.ge.13.120179.001535
    60 https://doi.org/10.1146/annurev.mi.35.100181.002053
    61 schema:datePublished 1985-04
    62 schema:datePublishedReg 1985-04-01
    63 schema:description Promoter-probe plasmid vectors were used to isolate putative promoter-containing DNA fragments of three Streptomyces antibiotic resistance genes, the rRNA methylase (tsr) gene of S. azureus, the aminoglycoside phosphotransferase (aph) gene of S. fradiae, and the viomycin phosphotransferase (vph) gene of S. vinaceus. DNA sequence analysis was carried out for all three of the fragments and for the protein-coding regions of the tsr and vph genes. No sequences resembling typical E. coli promoters or Bacillus vegetatively-expressed promoters were identified. Furthermore, none of the three DNA fragments found to be transcriptionally active in Streptomyces could initiate transcription when introduced into E. coli. An extremely biased codon usage pattern that reflects the high G + C composition of Streptomyces DNA was observed for the protein-coding regions of the tsr and vph genes, and of the previously sequenced aph gene. This pattern enabled delineation of the protein-coding region and identification of the coding strand of the genes.
    64 schema:genre research_article
    65 schema:inLanguage en
    66 schema:isAccessibleForFree false
    67 schema:isPartOf N451c4fc39f644ef48688be11ba48a587
    68 N792539d87d36426a97551513b19e7f9a
    69 sg:journal.1297380
    70 schema:name Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces
    71 schema:pagination 26-36
    72 schema:productId N300a7cc47776480c82222aa9775c228d
    73 N4d5e9a059dd3403cb0c0672ee9ce00d8
    74 N7c1d4b7186ff4180a56e259e7932ed50
    75 Ne00bab62e6d24cf985b5544a510b5644
    76 Ne5ea5923e92242ffb6e111d4522cec2d
    77 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039618263
    78 https://doi.org/10.1007/bf00327505
    79 schema:sdDatePublished 2019-04-15T08:50
    80 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    81 schema:sdPublisher N96f4a6e9c0af433188268af745a21d74
    82 schema:url http://link.springer.com/10.1007/BF00327505
    83 sgo:license sg:explorer/license/
    84 sgo:sdDataset articles
    85 rdf:type schema:ScholarlyArticle
    86 N03773a66a4604ff68f113db6bff90563 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    87 schema:name Promoter Regions, Genetic
    88 rdf:type schema:DefinedTerm
    89 N179cb77702334c688db2ad4286311778 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Streptomyces
    91 rdf:type schema:DefinedTerm
    92 N280ce2b7c2024ee78a24ba13125b8d68 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Transcription, Genetic
    94 rdf:type schema:DefinedTerm
    95 N300a7cc47776480c82222aa9775c228d schema:name doi
    96 schema:value 10.1007/bf00327505
    97 rdf:type schema:PropertyValue
    98 N35487ee4ff854e0993b6dc8c641082f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name DNA, Bacterial
    100 rdf:type schema:DefinedTerm
    101 N37c4a7b6daee4fe08310243722d4cccf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Escherichia coli
    103 rdf:type schema:DefinedTerm
    104 N388a7ef40dcc442c853687d3d77b8c03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Species Specificity
    106 rdf:type schema:DefinedTerm
    107 N40841c666d504da88222fbcccbc17fa8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Gene Expression Regulation
    109 rdf:type schema:DefinedTerm
    110 N451c4fc39f644ef48688be11ba48a587 schema:issueNumber 1
    111 rdf:type schema:PublicationIssue
    112 N4d5e9a059dd3403cb0c0672ee9ce00d8 schema:name pubmed_id
    113 schema:value 2987648
    114 rdf:type schema:PropertyValue
    115 N6562d5160fb041289a077df1b607577b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Drug Resistance, Microbial
    117 rdf:type schema:DefinedTerm
    118 N792539d87d36426a97551513b19e7f9a schema:volumeNumber 199
    119 rdf:type schema:PublicationVolume
    120 N7c1d4b7186ff4180a56e259e7932ed50 schema:name dimensions_id
    121 schema:value pub.1039618263
    122 rdf:type schema:PropertyValue
    123 N8f1124965f2542dd833a521cd1e2ae6a rdf:first sg:person.01263331517.27
    124 rdf:rest rdf:nil
    125 N96f4a6e9c0af433188268af745a21d74 schema:name Springer Nature - SN SciGraph project
    126 rdf:type schema:Organization
    127 N9b87e84fa0974676a9f7fe43848d20a8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Kanamycin Kinase
    129 rdf:type schema:DefinedTerm
    130 N9c7a61152067482ebfa701d8a8e22fae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    131 schema:name Phosphotransferases
    132 rdf:type schema:DefinedTerm
    133 Na342f30ae25c42a0b08f67cc3c7e18f0 rdf:first sg:person.014221666144.22
    134 rdf:rest Na89c16535b9e4c1aaff4b56f8888f09b
    135 Na89c16535b9e4c1aaff4b56f8888f09b rdf:first sg:person.01073762703.99
    136 rdf:rest N8f1124965f2542dd833a521cd1e2ae6a
    137 Nb2d90029962e48e2a93f1fb07c4404d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Base Sequence
    139 rdf:type schema:DefinedTerm
    140 Nb72927ef4f9b46f18ad95e6219783698 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Codon
    142 rdf:type schema:DefinedTerm
    143 Ncd1bc8eb0539404d8a53fcda1c435fa2 rdf:first sg:person.0612653332.06
    144 rdf:rest Na342f30ae25c42a0b08f67cc3c7e18f0
    145 Nda71ec1b59284bf6a1d3dec2cb757d90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Genes, Bacterial
    147 rdf:type schema:DefinedTerm
    148 Ne00bab62e6d24cf985b5544a510b5644 schema:name readcube_id
    149 schema:value bcb8e8c92489952cae1acf62b909ed77957cefd8d35b941d0154b20975fc3b84
    150 rdf:type schema:PropertyValue
    151 Ne0688890879e4db49e65d7944b465825 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name Methyltransferases
    153 rdf:type schema:DefinedTerm
    154 Ne5ea5923e92242ffb6e111d4522cec2d schema:name nlm_unique_id
    155 schema:value 0125036
    156 rdf:type schema:PropertyValue
    157 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    158 schema:name Biological Sciences
    159 rdf:type schema:DefinedTerm
    160 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    161 schema:name Genetics
    162 rdf:type schema:DefinedTerm
    163 sg:grant.2449214 http://pending.schema.org/fundedItem sg:pub.10.1007/bf00327505
    164 rdf:type schema:MonetaryGrant
    165 sg:grant.2633631 http://pending.schema.org/fundedItem sg:pub.10.1007/bf00327505
    166 rdf:type schema:MonetaryGrant
    167 sg:journal.1297380 schema:issn 1432-1874
    168 1617-4615
    169 schema:name Molecular Genetics and Genomics
    170 rdf:type schema:Periodical
    171 sg:person.01073762703.99 schema:affiliation https://www.grid.ac/institutes/grid.14830.3e
    172 schema:familyName Ward
    173 schema:givenName Judy M.
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073762703.99
    175 rdf:type schema:Person
    176 sg:person.01263331517.27 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    177 schema:familyName Cohen
    178 schema:givenName Stanley N.
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263331517.27
    180 rdf:type schema:Person
    181 sg:person.014221666144.22 schema:affiliation https://www.grid.ac/institutes/grid.14830.3e
    182 schema:familyName Bibb
    183 schema:givenName Maureen J.
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014221666144.22
    185 rdf:type schema:Person
    186 sg:person.0612653332.06 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    187 schema:familyName Bibb
    188 schema:givenName Mervyn J.
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612653332.06
    190 rdf:type schema:Person
    191 sg:pub.10.1007/978-3-642-68315-2_5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1081656921
    192 https://doi.org/10.1007/978-3-642-68315-2_5
    193 rdf:type schema:CreativeWork
    194 sg:pub.10.1007/bf00267782 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046612795
    195 https://doi.org/10.1007/bf00267782
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1007/bf00267869 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045931431
    198 https://doi.org/10.1007/bf00267869
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1007/bf00330791 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015265907
    201 https://doi.org/10.1007/bf00330791
    202 rdf:type schema:CreativeWork
    203 sg:pub.10.1007/bf00330831 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029070617
    204 https://doi.org/10.1007/bf00330831
    205 rdf:type schema:CreativeWork
    206 sg:pub.10.1007/bf00331128 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035940821
    207 https://doi.org/10.1007/bf00331128
    208 rdf:type schema:CreativeWork
    209 sg:pub.10.1007/bf00332721 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023826417
    210 https://doi.org/10.1007/bf00332721
    211 rdf:type schema:CreativeWork
    212 sg:pub.10.1007/bf02425325 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029286084
    213 https://doi.org/10.1007/bf02425325
    214 rdf:type schema:CreativeWork
    215 sg:pub.10.1038/268171a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041989527
    216 https://doi.org/10.1038/268171a0
    217 rdf:type schema:CreativeWork
    218 sg:pub.10.1038/274398a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037819258
    219 https://doi.org/10.1038/274398a0
    220 rdf:type schema:CreativeWork
    221 sg:pub.10.1038/284526a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035072504
    222 https://doi.org/10.1038/284526a0
    223 rdf:type schema:CreativeWork
    224 sg:pub.10.1038/286525a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022878906
    225 https://doi.org/10.1038/286525a0
    226 rdf:type schema:CreativeWork
    227 sg:pub.10.1038/313022a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041065614
    228 https://doi.org/10.1038/313022a0
    229 rdf:type schema:CreativeWork
    230 sg:pub.10.1038/newbio246040a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018988773
    231 https://doi.org/10.1038/newbio246040a0
    232 rdf:type schema:CreativeWork
    233 https://app.dimensions.ai/details/publication/pub.1080157422 schema:CreativeWork
    234 https://app.dimensions.ai/details/publication/pub.1080457401 schema:CreativeWork
    235 https://app.dimensions.ai/details/publication/pub.1080645369 schema:CreativeWork
    236 https://app.dimensions.ai/details/publication/pub.1081661037 schema:CreativeWork
    237 https://app.dimensions.ai/details/publication/pub.1081661038 schema:CreativeWork
    238 https://app.dimensions.ai/details/publication/pub.1081963372 schema:CreativeWork
    239 https://app.dimensions.ai/details/publication/pub.1082099856 schema:CreativeWork
    240 https://doi.org/10.1016/0022-2836(74)90357-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019434826
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1016/0378-1119(82)90086-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012114442
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1016/0378-1119(83)90174-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021221912
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1016/0378-1119(84)90090-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046284362
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1016/0378-1119(84)90116-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051432348
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1016/b978-0-12-372380-2.50008-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045923569
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1016/s0076-6879(80)65055-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051624275
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1016/s0076-6879(80)65059-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008420927
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1016/s0968-0004(78)95527-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047328323
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1042/bj1040258 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043664260
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1073/pnas.69.8.2110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038510205
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1073/pnas.70.11.3240 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041686183
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1073/pnas.71.4.1342 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048283731
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1073/pnas.75.3.1433 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008922793
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1073/pnas.77.9.5201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024100683
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1073/pnas.80.17.5190 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049963307
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1093/nar/11.8.2237 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035258827
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1093/nar/7.6.1513 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046335196
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1099/00221287-129-9-2703 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060364998
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1099/00221287-80-2-389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060375149
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1146/annurev.ge.13.120179.001535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005203403
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1146/annurev.mi.35.100181.002053 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009116890
    283 rdf:type schema:CreativeWork
    284 https://www.grid.ac/institutes/grid.14830.3e schema:alternateName John Innes Centre
    285 schema:name John Innes Institute, Colney Lane, NR4 7UH, Norwich, United Kingdom
    286 rdf:type schema:Organization
    287 https://www.grid.ac/institutes/grid.168010.e schema:alternateName Stanford University
    288 schema:name Department of Genetics, Stanford University School of Medicine, 94305, Stanford, CA, USA
    289 John Innes Institute, Colney Lane, NR4 7UH, Norwich, United Kingdom
    290 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...